Crystallization and preliminary X-ray analyses of desulfurization enzyme DszB and its C27S mutant complexed with biphenyl-2-sulfinic acid

DszB is a hydrolase involved in the biodegradation of dibenzothiophene in the soil bacterium Rhodococcus sp. IGTS8. DszB catalyzes the hydrolysis of 2′‐hydroxybiphenyl‐2‐sulfinic acid to sulfite and biphenyl‐2‐ol. DszB and DszB C27S mutant complexed with biphenyl‐2‐sulfinic acid were crystallized an...

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Veröffentlicht in:	Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2004-09, Vol.60 (9), p.1636-1638
Hauptverfasser:	Lee, Woo Cheol, Ohshiro, Takashi, Matsubara, Toshiyuki, Izumi, Yoshikazu, Tanokura, Masaru
Format:	Artikel
Sprache:	eng
Schlagworte:	Biphenyl Compounds - chemistry Crystallization dibenzothiophene DszB Escherichia coli - chemistry Esterases - chemistry hydrolase Indicators and Reagents Mutation - genetics Rhodococcus - enzymology Sulfinic Acids - chemistry X-Ray Diffraction
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container_title	Acta crystallographica. Section D, Biological crystallography.
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creator	Lee, Woo Cheol Ohshiro, Takashi Matsubara, Toshiyuki Izumi, Yoshikazu Tanokura, Masaru
description	DszB is a hydrolase involved in the biodegradation of dibenzothiophene in the soil bacterium Rhodococcus sp. IGTS8. DszB catalyzes the hydrolysis of 2′‐hydroxybiphenyl‐2‐sulfinic acid to sulfite and biphenyl‐2‐ol. DszB and DszB C27S mutant complexed with biphenyl‐2‐sulfinic acid were crystallized and preliminary X‐ray crystallographic analyses were conducted. The crystals of DszB were found to belong to the orthorhombic P212121 space group, with unit‐cell parameters a = 36.7, b = 82.6, c = 139.6 Å, and to contain one molecule of DszB in the asymmetric unit. Crystals of DszB C27S complexed with biphenyl‐2‐sulfinic acid belong to space group C2, with unit‐cell parameters a = 153.4, b = 45.9, c = 112.9 Å, β = 115.93°. The calculated Matthews coefficient VM for the C2 crystals is approximately 2.3 Å3 Da−1 if two molecules of DszB are present in the asymmetric unit.
doi_str_mv	10.1107/S0907444904017627
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Section D, Biological crystallography.</title><addtitle>Acta Cryst. D</addtitle><description>DszB is a hydrolase involved in the biodegradation of dibenzothiophene in the soil bacterium Rhodococcus sp. IGTS8. DszB catalyzes the hydrolysis of 2′‐hydroxybiphenyl‐2‐sulfinic acid to sulfite and biphenyl‐2‐ol. DszB and DszB C27S mutant complexed with biphenyl‐2‐sulfinic acid were crystallized and preliminary X‐ray crystallographic analyses were conducted. The crystals of DszB were found to belong to the orthorhombic P212121 space group, with unit‐cell parameters a = 36.7, b = 82.6, c = 139.6 Å, and to contain one molecule of DszB in the asymmetric unit. Crystals of DszB C27S complexed with biphenyl‐2‐sulfinic acid belong to space group C2, with unit‐cell parameters a = 153.4, b = 45.9, c = 112.9 Å, β = 115.93°. The calculated Matthews coefficient VM for the C2 crystals is approximately 2.3 Å3 Da−1 if two molecules of DszB are present in the asymmetric unit.</description><subject>Biphenyl Compounds - chemistry</subject><subject>Crystallization</subject><subject>dibenzothiophene</subject><subject>DszB</subject><subject>Escherichia coli - chemistry</subject><subject>Esterases - chemistry</subject><subject>hydrolase</subject><subject>Indicators and Reagents</subject><subject>Mutation - genetics</subject><subject>Rhodococcus - enzymology</subject><subject>Sulfinic Acids - chemistry</subject><subject>X-Ray Diffraction</subject><issn>1399-0047</issn><issn>0907-4449</issn><issn>1399-0047</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAURi1ERUvLD2CDvGIXuH7ETpZlClOktqidQcDKcpIb1eA8aidqM_-Af03aGR4Si658ZZ1zFh8hLxm8YQz02xXkoKWUOUhgWnH9hBwwkecJgNRP_7n3yfMYvwMA50I_I_ssFULkEg7Iz0WY4mC9dxs7uK6ltq1oH9C7xrU2TPRrEuw0_1o_RYy0q2mFcfT1GH4b2G6mBulJ3Lx7sN0Q6YLrFW3GwbYDLbum93iHFb11wzUtXH-N7eQTntx3XOtKaktXHZG92vqIL3bvIfn84f16cZqcfVp-XByfJaXIlE44VFKlhVW6YkphhQAqz7hUdWotghZ5AboobIFYIk8zW-Y6lTLL6kJyhVIcktfbbh-6mxHjYBoXS_TettiN0SiVcZ6ybAbZFixDF2PA2vTBNfMmhoG539_8t__svNrFx6LB6q-xG3wGsi1w6zxOjxfN8beT9SqFh3ayVV0c8O6PasMPo7TQqflysTSX5xfrK708NUz8AsMhocQ</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Lee, Woo Cheol</creator><creator>Ohshiro, Takashi</creator><creator>Matsubara, Toshiyuki</creator><creator>Izumi, Yoshikazu</creator><creator>Tanokura, Masaru</creator><general>Munksgaard International Publishers</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>Crystallization and preliminary X-ray analyses of desulfurization enzyme DszB and its C27S mutant complexed with biphenyl-2-sulfinic acid</title><author>Lee, Woo Cheol ; Ohshiro, Takashi ; Matsubara, Toshiyuki ; Izumi, Yoshikazu ; Tanokura, Masaru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3867-20d465ba67d166ede00698246f5aae0739b07bbabeece258ac9754488fb426e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biphenyl Compounds - chemistry</topic><topic>Crystallization</topic><topic>dibenzothiophene</topic><topic>DszB</topic><topic>Escherichia coli - chemistry</topic><topic>Esterases - chemistry</topic><topic>hydrolase</topic><topic>Indicators and Reagents</topic><topic>Mutation - genetics</topic><topic>Rhodococcus - enzymology</topic><topic>Sulfinic Acids - chemistry</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Woo Cheol</creatorcontrib><creatorcontrib>Ohshiro, Takashi</creatorcontrib><creatorcontrib>Matsubara, Toshiyuki</creatorcontrib><creatorcontrib>Izumi, Yoshikazu</creatorcontrib><creatorcontrib>Tanokura, Masaru</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta crystallographica. Section D, Biological crystallography.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Woo Cheol</au><au>Ohshiro, Takashi</au><au>Matsubara, Toshiyuki</au><au>Izumi, Yoshikazu</au><au>Tanokura, Masaru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crystallization and preliminary X-ray analyses of desulfurization enzyme DszB and its C27S mutant complexed with biphenyl-2-sulfinic acid</atitle><jtitle>Acta crystallographica. Section D, Biological crystallography.</jtitle><addtitle>Acta Cryst. D</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>60</volume><issue>9</issue><spage>1636</spage><epage>1638</epage><pages>1636-1638</pages><issn>1399-0047</issn><issn>0907-4449</issn><eissn>1399-0047</eissn><abstract>DszB is a hydrolase involved in the biodegradation of dibenzothiophene in the soil bacterium Rhodococcus sp. IGTS8. 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subjects	Biphenyl Compounds - chemistry Crystallization dibenzothiophene DszB Escherichia coli - chemistry Esterases - chemistry hydrolase Indicators and Reagents Mutation - genetics Rhodococcus - enzymology Sulfinic Acids - chemistry X-Ray Diffraction
title	Crystallization and preliminary X-ray analyses of desulfurization enzyme DszB and its C27S mutant complexed with biphenyl-2-sulfinic acid
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