Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: A novel cancer biomarker
Abstract The present study aimed to describe the characterization of an antibody MGb2 that reacts with an epitope on gastric cancer cells, and identification of MGb2 antigen (MGb2-Ag). Immunostaining revealed its distribution in human tissues and demonstrated that the positive rate of MGb2-Ag was 81...
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| Veröffentlicht in: | Cancer letters 2009-02, Vol.274 (2), p.250-258 |
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| container_title | Cancer letters |
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| creator | Zhang, Faming Ren, Gui Lu, Yuanyuan Jin, Bin Wang, Jun Chen, Xiong Liu, Zhenxiong Li, Kai Nie, Yongzhan Wang, Xin Fan, Daiming |
| description | Abstract The present study aimed to describe the characterization of an antibody MGb2 that reacts with an epitope on gastric cancer cells, and identification of MGb2 antigen (MGb2-Ag). Immunostaining revealed its distribution in human tissues and demonstrated that the positive rate of MGb2-Ag was 81.48% in gastric cancer, 100% in gastric signet-ring cell carcinoma and mucinous adenocarcinoma, 13.16% in precancerous conditions, and 0% in chronic superficial gastritis. Using Western blotting, immunoprecipitation and MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight mass spectrometry), MGb2-Ag was identified as TRAK1 (Trafficking protein, kinesin-binding 1), a new molecular gained limited recognition. Both MGb2 and commercial anti-TRAK1 Ab recognized prokaryotic expressed TRAK1. Immunostaining characteristics of TRAK1 were identical with MGb2-Ag in continuous sections of paraffin-embedded tissues of gastric tissues. This is the first report that TRAK1/MGb2-Ag is a promising diagnostic marker for gastric cancer and may help to detect signet-ring cell carcinoma and mucinous adenocarcinoma. |
| doi_str_mv | 10.1016/j.canlet.2008.09.031 |
| format | Article |
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Immunostaining revealed its distribution in human tissues and demonstrated that the positive rate of MGb2-Ag was 81.48% in gastric cancer, 100% in gastric signet-ring cell carcinoma and mucinous adenocarcinoma, 13.16% in precancerous conditions, and 0% in chronic superficial gastritis. Using Western blotting, immunoprecipitation and MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight mass spectrometry), MGb2-Ag was identified as TRAK1 (Trafficking protein, kinesin-binding 1), a new molecular gained limited recognition. Both MGb2 and commercial anti-TRAK1 Ab recognized prokaryotic expressed TRAK1. Immunostaining characteristics of TRAK1 were identical with MGb2-Ag in continuous sections of paraffin-embedded tissues of gastric tissues. This is the first report that TRAK1/MGb2-Ag is a promising diagnostic marker for gastric cancer and may help to detect signet-ring cell carcinoma and mucinous adenocarcinoma.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2008.09.031</identifier><identifier>PMID: 18986759</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adaptor Proteins, Vesicular Transport - metabolism ; Antibodies - metabolism ; Bioinformatics ; Biomarkers ; Biomarkers, Tumor - metabolism ; Blotting, Western ; Cancer biomarker ; Cell Line, Tumor ; Electrophoresis, Polyacrylamide Gel ; Female ; Gastric cancer ; Gastrointestinal cancer ; Hematology, Oncology and Palliative Medicine ; Humans ; Immunohistochemistry ; Immunoprecipitation ; Infections ; Male ; MGb2-Ag ; Middle Aged ; Mortality ; Neurosciences ; Peptides ; Signet-ring cell ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology ; TRAK1</subject><ispartof>Cancer letters, 2009-02, Vol.274 (2), p.250-258</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2008 Elsevier Ireland Ltd</rights><rights>Copyright Elsevier Limited Feb 18, 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-b7f7a17351b7b7b9d868fe44765733df7ef47947716e9e544f7eb9e6a17331823</citedby><cites>FETCH-LOGICAL-c443t-b7f7a17351b7b7b9d868fe44765733df7ef47947716e9e544f7eb9e6a17331823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304383508007945$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18986759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Faming</creatorcontrib><creatorcontrib>Ren, Gui</creatorcontrib><creatorcontrib>Lu, Yuanyuan</creatorcontrib><creatorcontrib>Jin, Bin</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><creatorcontrib>Chen, Xiong</creatorcontrib><creatorcontrib>Liu, Zhenxiong</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Nie, Yongzhan</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Fan, Daiming</creatorcontrib><title>Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: A novel cancer biomarker</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Abstract The present study aimed to describe the characterization of an antibody MGb2 that reacts with an epitope on gastric cancer cells, and identification of MGb2 antigen (MGb2-Ag). Immunostaining revealed its distribution in human tissues and demonstrated that the positive rate of MGb2-Ag was 81.48% in gastric cancer, 100% in gastric signet-ring cell carcinoma and mucinous adenocarcinoma, 13.16% in precancerous conditions, and 0% in chronic superficial gastritis. Using Western blotting, immunoprecipitation and MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight mass spectrometry), MGb2-Ag was identified as TRAK1 (Trafficking protein, kinesin-binding 1), a new molecular gained limited recognition. Both MGb2 and commercial anti-TRAK1 Ab recognized prokaryotic expressed TRAK1. Immunostaining characteristics of TRAK1 were identical with MGb2-Ag in continuous sections of paraffin-embedded tissues of gastric tissues. This is the first report that TRAK1/MGb2-Ag is a promising diagnostic marker for gastric cancer and may help to detect signet-ring cell carcinoma and mucinous adenocarcinoma.</description><subject>Adaptor Proteins, Vesicular Transport - metabolism</subject><subject>Antibodies - metabolism</subject><subject>Bioinformatics</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Blotting, Western</subject><subject>Cancer biomarker</subject><subject>Cell Line, Tumor</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Gastric cancer</subject><subject>Gastrointestinal cancer</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunoprecipitation</subject><subject>Infections</subject><subject>Male</subject><subject>MGb2-Ag</subject><subject>Middle Aged</subject><subject>Mortality</subject><subject>Neurosciences</subject><subject>Peptides</subject><subject>Signet-ring cell</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><subject>TRAK1</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUtuKFDEQDaK44-gfiAQEUdhuk87dB2FY3Au7Iuj4HPpSvWSmJ72b9Czs31vNDCzsi-QhpHLqVNU5Rch7zkrOuP66Kds6DjCVFWO2ZK5kgr8gC25NVRhn2UuyYILJQlihTsibnDeMMSWNek1OuHVWG-UWBK46iFPoQ1tPYYx07On69-qa08_rVPcY3oZ4S-_SOEGIpxRfkEMsmhC7-YN_oXWmPy-aqljdfqMrGscHGCh21kKiTRh3ddpCekte9fWQ4d3xXpK_5z_WZ5fFza-Lq7PVTdFKKaaiMb2puRGKNwaP66y2PUhptDJCdL2BXhonjeEaHCgpMdI40HOO4LYSS_LpwIsN3-8hT34XcgvDUEcY99lrbblzRiPw4zPgZtyniL15rlAlyxhyLok8oNo05pyg93cp4ESPnjM_m-A3_mCCn03wzHk0AdM-HMn3zQ66p6Sj6gj4fgAAavEQIPncBkDJupCgnXw3hv9VeE7QDiGih8MWHiE_zeJz5Zn_My_CvAcM50IBlfgHSiyr6g</recordid><startdate>20090218</startdate><enddate>20090218</enddate><creator>Zhang, Faming</creator><creator>Ren, Gui</creator><creator>Lu, Yuanyuan</creator><creator>Jin, Bin</creator><creator>Wang, Jun</creator><creator>Chen, Xiong</creator><creator>Liu, Zhenxiong</creator><creator>Li, Kai</creator><creator>Nie, Yongzhan</creator><creator>Wang, Xin</creator><creator>Fan, Daiming</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20090218</creationdate><title>Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: A novel cancer biomarker</title><author>Zhang, Faming ; Ren, Gui ; Lu, Yuanyuan ; Jin, Bin ; Wang, Jun ; Chen, Xiong ; Liu, Zhenxiong ; Li, Kai ; Nie, Yongzhan ; Wang, Xin ; Fan, Daiming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-b7f7a17351b7b7b9d868fe44765733df7ef47947716e9e544f7eb9e6a17331823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adaptor Proteins, Vesicular Transport - metabolism</topic><topic>Antibodies - metabolism</topic><topic>Bioinformatics</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Blotting, Western</topic><topic>Cancer biomarker</topic><topic>Cell Line, Tumor</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Gastric cancer</topic><topic>Gastrointestinal cancer</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunoprecipitation</topic><topic>Infections</topic><topic>Male</topic><topic>MGb2-Ag</topic><topic>Middle Aged</topic><topic>Mortality</topic><topic>Neurosciences</topic><topic>Peptides</topic><topic>Signet-ring cell</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><topic>TRAK1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Faming</creatorcontrib><creatorcontrib>Ren, Gui</creatorcontrib><creatorcontrib>Lu, Yuanyuan</creatorcontrib><creatorcontrib>Jin, Bin</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><creatorcontrib>Chen, Xiong</creatorcontrib><creatorcontrib>Liu, Zhenxiong</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Nie, Yongzhan</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Fan, Daiming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Faming</au><au>Ren, Gui</au><au>Lu, Yuanyuan</au><au>Jin, Bin</au><au>Wang, Jun</au><au>Chen, Xiong</au><au>Liu, Zhenxiong</au><au>Li, Kai</au><au>Nie, Yongzhan</au><au>Wang, Xin</au><au>Fan, Daiming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: A novel cancer biomarker</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2009-02-18</date><risdate>2009</risdate><volume>274</volume><issue>2</issue><spage>250</spage><epage>258</epage><pages>250-258</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>Abstract The present study aimed to describe the characterization of an antibody MGb2 that reacts with an epitope on gastric cancer cells, and identification of MGb2 antigen (MGb2-Ag). Immunostaining revealed its distribution in human tissues and demonstrated that the positive rate of MGb2-Ag was 81.48% in gastric cancer, 100% in gastric signet-ring cell carcinoma and mucinous adenocarcinoma, 13.16% in precancerous conditions, and 0% in chronic superficial gastritis. Using Western blotting, immunoprecipitation and MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight mass spectrometry), MGb2-Ag was identified as TRAK1 (Trafficking protein, kinesin-binding 1), a new molecular gained limited recognition. Both MGb2 and commercial anti-TRAK1 Ab recognized prokaryotic expressed TRAK1. Immunostaining characteristics of TRAK1 were identical with MGb2-Ag in continuous sections of paraffin-embedded tissues of gastric tissues. This is the first report that TRAK1/MGb2-Ag is a promising diagnostic marker for gastric cancer and may help to detect signet-ring cell carcinoma and mucinous adenocarcinoma.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>18986759</pmid><doi>10.1016/j.canlet.2008.09.031</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0304-3835 |
| ispartof | Cancer letters, 2009-02, Vol.274 (2), p.250-258 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adaptor Proteins, Vesicular Transport - metabolism Antibodies - metabolism Bioinformatics Biomarkers Biomarkers, Tumor - metabolism Blotting, Western Cancer biomarker Cell Line, Tumor Electrophoresis, Polyacrylamide Gel Female Gastric cancer Gastrointestinal cancer Hematology, Oncology and Palliative Medicine Humans Immunohistochemistry Immunoprecipitation Infections Male MGb2-Ag Middle Aged Mortality Neurosciences Peptides Signet-ring cell Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Stomach Neoplasms - metabolism Stomach Neoplasms - pathology TRAK1 |
| title | Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: A novel cancer biomarker |
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