Expression of the Peptidoglycan Recognition Protein, PGRP, in the Lactating Mammary Gland
The peptidoglycan recognition protein, PGRP, known as an intracellular component of neutrophils, has been isolated from camel (Camelus dromedarius) milk by acid precipitation followed by heparin-sepharose affinity chromatography of the supernatant. The mean concentration in milk was about 120mg/L. I...
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| Veröffentlicht in: | Journal of dairy science 2004-08, Vol.87 (8), p.2660-2668 |
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| description | The peptidoglycan recognition protein, PGRP, known as an intracellular component of neutrophils, has been isolated from camel (Camelus dromedarius) milk by acid precipitation followed by heparin-sepharose affinity chromatography of the supernatant. The mean concentration in milk was about 120mg/L. It decreased during lactation by 19% and increased in the event of severe mastitis by 45%. The protein bound to lactic acid bacteria and other gram-positive bacteria with an affinity similar to that reported for the human and murine orthologs, although the isoelectric point of the molecule was distinctly higher at pH 9.02. The N-terminus of mature camel PGRP was determined as NH2-ArgGluAspProPro-CO2H. Calculated and measured molecular masses were both 19.1kDa, excluding the possibility of posttranslational modifcation or binding of cation ligands. The peptide probably builds a homotrimer at high concentration. The corresponding mRNA was isolated from lactating mammary gland tissue, and 5.3 kbp of the corresponding gene was sequenced. Similarities were found to the camel lactoferrin gene with regard to sites of expression and to the region 5′ upstream to the gene. |
| doi_str_mv | 10.3168/jds.S0022-0302(04)73392-5 |
| format | Article |
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The mean concentration in milk was about 120mg/L. It decreased during lactation by 19% and increased in the event of severe mastitis by 45%. The protein bound to lactic acid bacteria and other gram-positive bacteria with an affinity similar to that reported for the human and murine orthologs, although the isoelectric point of the molecule was distinctly higher at pH 9.02. The N-terminus of mature camel PGRP was determined as NH2-ArgGluAspProPro-CO2H. Calculated and measured molecular masses were both 19.1kDa, excluding the possibility of posttranslational modifcation or binding of cation ligands. The peptide probably builds a homotrimer at high concentration. The corresponding mRNA was isolated from lactating mammary gland tissue, and 5.3 kbp of the corresponding gene was sequenced. Similarities were found to the camel lactoferrin gene with regard to sites of expression and to the region 5′ upstream to the gene.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.S0022-0302(04)73392-5</identifier><identifier>PMID: 15328291</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>Savoy, IL: Elsevier Inc</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animal productions ; Animals ; antibacterial properties ; Base Sequence ; binding proteins ; Biological and medical sciences ; camel milk ; Camelus - genetics ; Carrier Proteins - analysis ; Carrier Proteins - chemistry ; Carrier Proteins - genetics ; DNA, Complementary - chemistry ; dromedaries ; Female ; Food industries ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; gene expression regulation ; Isoelectric Point ; lactating mammary gland ; Lactation ; mammary gland function ; mammary glands ; Mammary Glands, Animal - chemistry ; mastitis ; Micrococcus luteus - metabolism ; Milk - chemistry ; Milk and cheese industries. Ice creams ; Molecular Sequence Data ; Molecular Weight ; nucleotide sequences ; Peptide Fragments - chemistry ; peptidoglycan recognition protein ; purification ; RNA, Messenger - analysis ; RNA, Messenger - isolation & purification ; Sequence Analysis, DNA ; Sequence Analysis, Protein ; Staphylococcus aureus - metabolism ; Terrestrial animal productions ; Vertebrates</subject><ispartof>Journal of dairy science, 2004-08, Vol.87 (8), p.2660-2668</ispartof><rights>2004 American Dairy Science Association</rights><rights>2004 INIST-CNRS</rights><rights>Copyright American Dairy Science Association Aug 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-7b1e521dff7101f473abe5b1966c38275495928f41ac3c9efc306275b0998f023</citedby><cites>FETCH-LOGICAL-c536t-7b1e521dff7101f473abe5b1966c38275495928f41ac3c9efc306275b0998f023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3168/jds.S0022-0302(04)73392-5$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15980592$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15328291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kappeler, S.R.</creatorcontrib><creatorcontrib>Heuberger, C.</creatorcontrib><creatorcontrib>Farah, Z.</creatorcontrib><creatorcontrib>Puhan, Z.</creatorcontrib><title>Expression of the Peptidoglycan Recognition Protein, PGRP, in the Lactating Mammary Gland</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>The peptidoglycan recognition protein, PGRP, known as an intracellular component of neutrophils, has been isolated from camel (Camelus dromedarius) milk by acid precipitation followed by heparin-sepharose affinity chromatography of the supernatant. The mean concentration in milk was about 120mg/L. It decreased during lactation by 19% and increased in the event of severe mastitis by 45%. The protein bound to lactic acid bacteria and other gram-positive bacteria with an affinity similar to that reported for the human and murine orthologs, although the isoelectric point of the molecule was distinctly higher at pH 9.02. The N-terminus of mature camel PGRP was determined as NH2-ArgGluAspProPro-CO2H. Calculated and measured molecular masses were both 19.1kDa, excluding the possibility of posttranslational modifcation or binding of cation ligands. The peptide probably builds a homotrimer at high concentration. The corresponding mRNA was isolated from lactating mammary gland tissue, and 5.3 kbp of the corresponding gene was sequenced. Similarities were found to the camel lactoferrin gene with regard to sites of expression and to the region 5′ upstream to the gene.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animal productions</subject><subject>Animals</subject><subject>antibacterial properties</subject><subject>Base Sequence</subject><subject>binding proteins</subject><subject>Biological and medical sciences</subject><subject>camel milk</subject><subject>Camelus - genetics</subject><subject>Carrier Proteins - analysis</subject><subject>Carrier Proteins - chemistry</subject><subject>Carrier Proteins - genetics</subject><subject>DNA, Complementary - chemistry</subject><subject>dromedaries</subject><subject>Female</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>gene expression regulation</subject><subject>Isoelectric Point</subject><subject>lactating mammary gland</subject><subject>Lactation</subject><subject>mammary gland function</subject><subject>mammary glands</subject><subject>Mammary Glands, Animal - chemistry</subject><subject>mastitis</subject><subject>Micrococcus luteus - metabolism</subject><subject>Milk - chemistry</subject><subject>Milk and cheese industries. Ice creams</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>nucleotide sequences</subject><subject>Peptide Fragments - chemistry</subject><subject>peptidoglycan recognition protein</subject><subject>purification</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - isolation & purification</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Analysis, Protein</subject><subject>Staphylococcus aureus - metabolism</subject><subject>Terrestrial animal productions</subject><subject>Vertebrates</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkV1v0zAYhS0EYmXwFyAggUBahj9ix75E1ShIRVQbu-DKchw7dZXYnZ1u7N_jtBVDXHFl2Xre855zDMBrBM8JYvzjpk3nVxBiXEIC8XtYfagJEbikj8AMUUxLggR_DGZ_kBPwLKVNviIM6VNwgijBHAs0Az8vfm2jSckFXwRbjGtTrMx2dG3o-nutfHFpdOi8GydgFcNonD8rVovL1Vnh_J5fKj2q0fmu-KaGQcX7YtEr3z4HT6zqk3lxPE_B9eeLH_Mv5fL74uv807LUlLCxrBtkKEattTWCyFY1UY2hDRKMacJxTStBBea2QkoTLYzVBLL83EAhuIWYnIJ3B91tDDc7k0Y5uKRNnz2YsEuSMY4oZCSDb_4BN2EXffYmkaB5VUVhhsQB0jGkFI2V2-imUBJBOZUvc_lyX76cmpWwkvvyJc2zL48Lds1g2ofJY9sZeHsEVNKqt1F57dJfnOAwh32ItHbd-s5FI9Og-j7Lomk9ryWXmLHJ7asDaFWQqotZ7PoKQ0QgFBVh9UTMD4TJX3DrTJRJO-O1abOsHmUb3H8k-w0w5bfz</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Kappeler, S.R.</creator><creator>Heuberger, C.</creator><creator>Farah, Z.</creator><creator>Puhan, Z.</creator><general>Elsevier Inc</general><general>Am Dairy Sci Assoc</general><general>American Dairy Science Association</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Expression of the Peptidoglycan Recognition Protein, PGRP, in the Lactating Mammary Gland</title><author>Kappeler, S.R. ; Heuberger, C. ; Farah, Z. ; Puhan, Z.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-7b1e521dff7101f473abe5b1966c38275495928f41ac3c9efc306275b0998f023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animal productions</topic><topic>Animals</topic><topic>antibacterial properties</topic><topic>Base Sequence</topic><topic>binding proteins</topic><topic>Biological and medical sciences</topic><topic>camel milk</topic><topic>Camelus - genetics</topic><topic>Carrier Proteins - analysis</topic><topic>Carrier Proteins - chemistry</topic><topic>Carrier Proteins - genetics</topic><topic>DNA, Complementary - chemistry</topic><topic>dromedaries</topic><topic>Female</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>gene expression regulation</topic><topic>Isoelectric Point</topic><topic>lactating mammary gland</topic><topic>Lactation</topic><topic>mammary gland function</topic><topic>mammary glands</topic><topic>Mammary Glands, Animal - chemistry</topic><topic>mastitis</topic><topic>Micrococcus luteus - metabolism</topic><topic>Milk - chemistry</topic><topic>Milk and cheese industries. Ice creams</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>nucleotide sequences</topic><topic>Peptide Fragments - chemistry</topic><topic>peptidoglycan recognition protein</topic><topic>purification</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - isolation & purification</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Analysis, Protein</topic><topic>Staphylococcus aureus - metabolism</topic><topic>Terrestrial animal productions</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kappeler, S.R.</creatorcontrib><creatorcontrib>Heuberger, C.</creatorcontrib><creatorcontrib>Farah, Z.</creatorcontrib><creatorcontrib>Puhan, Z.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Engineering Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kappeler, S.R.</au><au>Heuberger, C.</au><au>Farah, Z.</au><au>Puhan, Z.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the Peptidoglycan Recognition Protein, PGRP, in the Lactating Mammary Gland</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>87</volume><issue>8</issue><spage>2660</spage><epage>2668</epage><pages>2660-2668</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><coden>JDSCAE</coden><abstract>The peptidoglycan recognition protein, PGRP, known as an intracellular component of neutrophils, has been isolated from camel (Camelus dromedarius) milk by acid precipitation followed by heparin-sepharose affinity chromatography of the supernatant. The mean concentration in milk was about 120mg/L. It decreased during lactation by 19% and increased in the event of severe mastitis by 45%. The protein bound to lactic acid bacteria and other gram-positive bacteria with an affinity similar to that reported for the human and murine orthologs, although the isoelectric point of the molecule was distinctly higher at pH 9.02. The N-terminus of mature camel PGRP was determined as NH2-ArgGluAspProPro-CO2H. Calculated and measured molecular masses were both 19.1kDa, excluding the possibility of posttranslational modifcation or binding of cation ligands. The peptide probably builds a homotrimer at high concentration. The corresponding mRNA was isolated from lactating mammary gland tissue, and 5.3 kbp of the corresponding gene was sequenced. Similarities were found to the camel lactoferrin gene with regard to sites of expression and to the region 5′ upstream to the gene.</abstract><cop>Savoy, IL</cop><pub>Elsevier Inc</pub><pmid>15328291</pmid><doi>10.3168/jds.S0022-0302(04)73392-5</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Sequence amino acid sequences Animal productions Animals antibacterial properties Base Sequence binding proteins Biological and medical sciences camel milk Camelus - genetics Carrier Proteins - analysis Carrier Proteins - chemistry Carrier Proteins - genetics DNA, Complementary - chemistry dromedaries Female Food industries Fundamental and applied biological sciences. Psychology Gene Expression gene expression regulation Isoelectric Point lactating mammary gland Lactation mammary gland function mammary glands Mammary Glands, Animal - chemistry mastitis Micrococcus luteus - metabolism Milk - chemistry Milk and cheese industries. Ice creams Molecular Sequence Data Molecular Weight nucleotide sequences Peptide Fragments - chemistry peptidoglycan recognition protein purification RNA, Messenger - analysis RNA, Messenger - isolation & purification Sequence Analysis, DNA Sequence Analysis, Protein Staphylococcus aureus - metabolism Terrestrial animal productions Vertebrates |
| title | Expression of the Peptidoglycan Recognition Protein, PGRP, in the Lactating Mammary Gland |
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