Arsenic complexes in the arsenic hyperaccumulator Pteris vittata (Chinese brake fern)
Pteris vittata (Chinese brake fern), the first reported arsenic (As) hyperaccumulating plant, can be potentially applied in the phytoremediation of As-contaminated sites. Understanding the mechanisms of As tolerance and detoxification in this plant is critical to further enhance its capability of As...
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| Veröffentlicht in: | Journal of Chromatography A 2004-07, Vol.1043 (2), p.249-254 |
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| creator | Zhang, Weihua Cai, Yong Downum, Kelsey R Ma, Lena Q |
| description | Pteris vittata (Chinese brake fern), the first reported arsenic (As) hyperaccumulating plant, can be potentially applied in the phytoremediation of As-contaminated sites. Understanding the mechanisms of As tolerance and detoxification in this plant is critical to further enhance its capability of As hyperaccumulation. In this study, an unknown As species, other than arsenite (As
III) or arsenate (As
V) was found in leaflets by using anion-exchange chromatography–hydride generation–atomic fluorescence spectroscopy and size-exclusion chromatography–atomic fluorescence spectrometry. The chromatographic behavior of this unknown As species and its stability suggest that it is likely an As complex. Although phytochelatin with two subunits (PC
2) was the only major thiol in
P. vittata under As exposure, this unknown As complex was unlikely to be an As
III–PC
2 complex by comparison of their chromatographic behaviors, stability at different pHs and charge states. The complex is sensitive to temperature and metal ions, but relatively insensitive to pH. In buffer solution of pH 5.9, it is present in a neutral form. |
| doi_str_mv | 10.1016/j.chroma.2004.05.090 |
| format | Article |
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III) or arsenate (As
V) was found in leaflets by using anion-exchange chromatography–hydride generation–atomic fluorescence spectroscopy and size-exclusion chromatography–atomic fluorescence spectrometry. The chromatographic behavior of this unknown As species and its stability suggest that it is likely an As complex. Although phytochelatin with two subunits (PC
2) was the only major thiol in
P. vittata under As exposure, this unknown As complex was unlikely to be an As
III–PC
2 complex by comparison of their chromatographic behaviors, stability at different pHs and charge states. The complex is sensitive to temperature and metal ions, but relatively insensitive to pH. In buffer solution of pH 5.9, it is present in a neutral form.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2004.05.090</identifier><identifier>PMID: 15330099</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical biochemistry: general aspects, technics, instrumentation ; Analytical chemistry ; Analytical, structural and metabolic biochemistry ; Arsenic ; Arsenicals - analysis ; Biological and medical sciences ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, Gel - methods ; Chromatography, Ion Exchange - methods ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Hydrogen-Ion Concentration ; Medical sciences ; Metal complexes ; Pharmacology. Drug treatments ; Phytochelatins ; Pteris - chemistry ; Pteris vittata ; Spectrometry, Fluorescence - methods ; Spectrophotometry, Atomic - methods</subject><ispartof>Journal of Chromatography A, 2004-07, Vol.1043 (2), p.249-254</ispartof><rights>2004 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-59a0c30a0cace954ef8468244eda99163a54fcddd926faffaa3a1765ab1f0b773</citedby><cites>FETCH-LOGICAL-c478t-59a0c30a0cace954ef8468244eda99163a54fcddd926faffaa3a1765ab1f0b773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chroma.2004.05.090$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15932608$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15330099$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Weihua</creatorcontrib><creatorcontrib>Cai, Yong</creatorcontrib><creatorcontrib>Downum, Kelsey R</creatorcontrib><creatorcontrib>Ma, Lena Q</creatorcontrib><title>Arsenic complexes in the arsenic hyperaccumulator Pteris vittata (Chinese brake fern)</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>Pteris vittata (Chinese brake fern), the first reported arsenic (As) hyperaccumulating plant, can be potentially applied in the phytoremediation of As-contaminated sites. Understanding the mechanisms of As tolerance and detoxification in this plant is critical to further enhance its capability of As hyperaccumulation. In this study, an unknown As species, other than arsenite (As
III) or arsenate (As
V) was found in leaflets by using anion-exchange chromatography–hydride generation–atomic fluorescence spectroscopy and size-exclusion chromatography–atomic fluorescence spectrometry. The chromatographic behavior of this unknown As species and its stability suggest that it is likely an As complex. Although phytochelatin with two subunits (PC
2) was the only major thiol in
P. vittata under As exposure, this unknown As complex was unlikely to be an As
III–PC
2 complex by comparison of their chromatographic behaviors, stability at different pHs and charge states. The complex is sensitive to temperature and metal ions, but relatively insensitive to pH. In buffer solution of pH 5.9, it is present in a neutral form.</description><subject>Analysis</subject><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical chemistry</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Arsenic</subject><subject>Arsenicals - analysis</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, Gel - methods</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Medical sciences</subject><subject>Metal complexes</subject><subject>Pharmacology. Drug treatments</subject><subject>Phytochelatins</subject><subject>Pteris - chemistry</subject><subject>Pteris vittata</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Spectrophotometry, Atomic - methods</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAQgH0A0VL4Bwh8AZXDhnGcxPEFqVqVh1QJJNizNeuMWS95LLZT0X-PV4kEJy4zh_nmoW8YeyGgECCad8fCHsI0YFECVAXUBWh4xC4BSrHRjZIX7GmMRwChQJVP2IWopQTQ-pLtbkKk0Vtup-HU02-K3I88HYjjWjg8nCigtfMw95imwL8mCj7ye58SJuTX24MfKRLfB_xJ3FEY3z5jjx32kZ6v-YrtPtx-337a3H35-Hl7c7exlWrTptYIVkIOaEnXFbm2atqyqqhDrUUjsa6c7bpOl41D5xAlCtXUuBcO9krJK_ZmmXsK06-ZYjKDj5b6Hkea5miaphVSiTaD1QLaMMUYyJlT8AOGByPAnBWao1kUmrNCA7XJCnPby3X-vB-o-9u0-svA6xXAaLF3AUfr4z-clmUD5_2vFs7hZPBH9md230oQEkR-EiiZifcLQdnXvadgovU0Wup8IJtMN_n_3_oHr9-dVw</recordid><startdate>20040723</startdate><enddate>20040723</enddate><creator>Zhang, Weihua</creator><creator>Cai, Yong</creator><creator>Downum, Kelsey R</creator><creator>Ma, Lena Q</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040723</creationdate><title>Arsenic complexes in the arsenic hyperaccumulator Pteris vittata (Chinese brake fern)</title><author>Zhang, Weihua ; Cai, Yong ; Downum, Kelsey R ; Ma, Lena Q</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-59a0c30a0cace954ef8468244eda99163a54fcddd926faffaa3a1765ab1f0b773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Analysis</topic><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical chemistry</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Arsenic</topic><topic>Arsenicals - analysis</topic><topic>Biological and medical sciences</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, Gel - methods</topic><topic>Chromatography, Ion Exchange - methods</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Medical sciences</topic><topic>Metal complexes</topic><topic>Pharmacology. Drug treatments</topic><topic>Phytochelatins</topic><topic>Pteris - chemistry</topic><topic>Pteris vittata</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Spectrophotometry, Atomic - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Weihua</creatorcontrib><creatorcontrib>Cai, Yong</creatorcontrib><creatorcontrib>Downum, Kelsey R</creatorcontrib><creatorcontrib>Ma, Lena Q</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Weihua</au><au>Cai, Yong</au><au>Downum, Kelsey R</au><au>Ma, Lena Q</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arsenic complexes in the arsenic hyperaccumulator Pteris vittata (Chinese brake fern)</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2004-07-23</date><risdate>2004</risdate><volume>1043</volume><issue>2</issue><spage>249</spage><epage>254</epage><pages>249-254</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>Pteris vittata (Chinese brake fern), the first reported arsenic (As) hyperaccumulating plant, can be potentially applied in the phytoremediation of As-contaminated sites. Understanding the mechanisms of As tolerance and detoxification in this plant is critical to further enhance its capability of As hyperaccumulation. In this study, an unknown As species, other than arsenite (As
III) or arsenate (As
V) was found in leaflets by using anion-exchange chromatography–hydride generation–atomic fluorescence spectroscopy and size-exclusion chromatography–atomic fluorescence spectrometry. The chromatographic behavior of this unknown As species and its stability suggest that it is likely an As complex. Although phytochelatin with two subunits (PC
2) was the only major thiol in
P. vittata under As exposure, this unknown As complex was unlikely to be an As
III–PC
2 complex by comparison of their chromatographic behaviors, stability at different pHs and charge states. The complex is sensitive to temperature and metal ions, but relatively insensitive to pH. In buffer solution of pH 5.9, it is present in a neutral form.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15330099</pmid><doi>10.1016/j.chroma.2004.05.090</doi><tpages>6</tpages></addata></record> |
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| subjects | Analysis Analytical biochemistry: general aspects, technics, instrumentation Analytical chemistry Analytical, structural and metabolic biochemistry Arsenic Arsenicals - analysis Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Gel - methods Chromatography, Ion Exchange - methods Exact sciences and technology Fundamental and applied biological sciences. Psychology General pharmacology Hydrogen-Ion Concentration Medical sciences Metal complexes Pharmacology. Drug treatments Phytochelatins Pteris - chemistry Pteris vittata Spectrometry, Fluorescence - methods Spectrophotometry, Atomic - methods |
| title | Arsenic complexes in the arsenic hyperaccumulator Pteris vittata (Chinese brake fern) |
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