Analysis of pentacyclic triterpenes by LC-MS. A comparative study between APCI and APPI
The analytical performances of three atmospheric-pressure sources, electrospray (ESI), atmospheric-pressure chemical ionization (APCI), and atmospheric-pressure photoionization (APPI), were evaluated for the analysis of pentacyclic triterpenes in liquid chromatography-mass spectrometry (LC-MS). Amon...
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| Veröffentlicht in: | Journal of mass spectrometry. 2009, Vol.44 (1), p.71-80 |
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| creator | Rhourri-Frih, B Chaimbault, P Claude, B Lamy, C André, P Lafosse, M |
| description | The analytical performances of three atmospheric-pressure sources, electrospray (ESI), atmospheric-pressure chemical ionization (APCI), and atmospheric-pressure photoionization (APPI), were evaluated for the analysis of pentacyclic triterpenes in liquid chromatography-mass spectrometry (LC-MS). Among these sources, APPI and APCI are particularly well adapted to sensitive analyses of pentacyclic triterpenes by LC-MS. Detection parameters were optimized for both the sources, and the effects of three dopants (toluene, acetone and anisole) on the detection (sensitivity and ion fingerprints in MS spectra) were studied in detail for APPI-MS.The limits of quantification were measured under selected ion monitoring conditions, in the range of 0.005-0.015 mg l⁻¹ and 0.002-0.84 mg l⁻¹ in APPI and APCI, respectively, depending on the studied pentacyclic triterpene. Overall, APPI was found more sensitive than APCI in positive ion mode, whereas APCI shows the greatest sensitivity for acidic triterpenes in negative ion mode.Following this study, the developed LC-MS method was used for the characterization of pentacyclic triterpenes in three plant extracts. High amounts of betulinic acid, betulinic aldehyde and betulinic aldehyde acetate were observed in plane bark. The main component of birch bark is betulin and extracts of okoume resin exhibit high amounts of α- and β-amyrin. Copyright © 2008 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/jms.1472 |
| format | Article |
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A comparative study between APCI and APPI</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Rhourri-Frih, B ; Chaimbault, P ; Claude, B ; Lamy, C ; André, P ; Lafosse, M</creator><creatorcontrib>Rhourri-Frih, B ; Chaimbault, P ; Claude, B ; Lamy, C ; André, P ; Lafosse, M</creatorcontrib><description>The analytical performances of three atmospheric-pressure sources, electrospray (ESI), atmospheric-pressure chemical ionization (APCI), and atmospheric-pressure photoionization (APPI), were evaluated for the analysis of pentacyclic triterpenes in liquid chromatography-mass spectrometry (LC-MS). Among these sources, APPI and APCI are particularly well adapted to sensitive analyses of pentacyclic triterpenes by LC-MS. Detection parameters were optimized for both the sources, and the effects of three dopants (toluene, acetone and anisole) on the detection (sensitivity and ion fingerprints in MS spectra) were studied in detail for APPI-MS.The limits of quantification were measured under selected ion monitoring conditions, in the range of 0.005-0.015 mg l⁻¹ and 0.002-0.84 mg l⁻¹ in APPI and APCI, respectively, depending on the studied pentacyclic triterpene. Overall, APPI was found more sensitive than APCI in positive ion mode, whereas APCI shows the greatest sensitivity for acidic triterpenes in negative ion mode.Following this study, the developed LC-MS method was used for the characterization of pentacyclic triterpenes in three plant extracts. High amounts of betulinic acid, betulinic aldehyde and betulinic aldehyde acetate were observed in plane bark. The main component of birch bark is betulin and extracts of okoume resin exhibit high amounts of α- and β-amyrin. 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A comparative study between APCI and APPI</title><title>Journal of mass spectrometry.</title><addtitle>J. Mass Spectrom</addtitle><description>The analytical performances of three atmospheric-pressure sources, electrospray (ESI), atmospheric-pressure chemical ionization (APCI), and atmospheric-pressure photoionization (APPI), were evaluated for the analysis of pentacyclic triterpenes in liquid chromatography-mass spectrometry (LC-MS). Among these sources, APPI and APCI are particularly well adapted to sensitive analyses of pentacyclic triterpenes by LC-MS. Detection parameters were optimized for both the sources, and the effects of three dopants (toluene, acetone and anisole) on the detection (sensitivity and ion fingerprints in MS spectra) were studied in detail for APPI-MS.The limits of quantification were measured under selected ion monitoring conditions, in the range of 0.005-0.015 mg l⁻¹ and 0.002-0.84 mg l⁻¹ in APPI and APCI, respectively, depending on the studied pentacyclic triterpene. Overall, APPI was found more sensitive than APCI in positive ion mode, whereas APCI shows the greatest sensitivity for acidic triterpenes in negative ion mode.Following this study, the developed LC-MS method was used for the characterization of pentacyclic triterpenes in three plant extracts. High amounts of betulinic acid, betulinic aldehyde and betulinic aldehyde acetate were observed in plane bark. The main component of birch bark is betulin and extracts of okoume resin exhibit high amounts of α- and β-amyrin. Copyright © 2008 John Wiley & Sons, Ltd.</description><subject>Acetone - chemistry</subject><subject>Analytical chemistry</subject><subject>Anisoles - chemistry</subject><subject>APCI</subject><subject>APPI</subject><subject>Atmospheric Pressure</subject><subject>Biological and medical sciences</subject><subject>Chemical constitution</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, Liquid - methods</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>LC-MS</subject><subject>Mass Spectrometry - methods</subject><subject>Other chromatographic methods</subject><subject>pentacyclic triterpenes</subject><subject>Pentacyclic Triterpenes - analysis</subject><subject>Plant Extracts - analysis</subject><subject>Plant physiology and development</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Toluene - chemistry</subject><issn>1076-5174</issn><issn>1096-9888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1v1DAQBuAIgWgpSPwC8AXEJYvtOP44LivYLtpCpaVaiYs1ccYoJR9bO0vJvyfRRuWEOM3IevSO9SbJS0YXjFL-_raJCyYUf5ScM2pkarTWj6ddyTRnSpwlz2K8pZQaI-TT5IzpcWplzpP9soV6iFUknScHbHtwg6srR_pQ9RjGF4ykGMh2lV7tFmRJXNccIEBf_UIS-2M5kAL7e8SWLK9XGwJtOS7Xm-fJEw91xBfzvEhuPn38trpMt1_Xm9VymzohOE-zAnNGJc1zp4pSahQetPSlkc6ABOVUrsFzbzwFVFlRClSgPJVGMyEozy6St6fcQ-jujhh721TRYV1Di90xWik140yY_8JMUmUMneC7E3ShizGgt4dQNRAGy6id2rZj23Zqe6Sv5sxj0WD5F871juDNDCA6qH2A1lXxwfExTmZycunJ3Vc1Dv88aD9f7ebDs69ij78fPISfVqpM5Xb_ZW0_7PVaXX7fWjn61yfvobPwI4x_uNlxyjLKcp1xLrM_Jr2sOQ</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Rhourri-Frih, B</creator><creator>Chaimbault, P</creator><creator>Claude, B</creator><creator>Lamy, C</creator><creator>André, P</creator><creator>Lafosse, M</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>2009</creationdate><title>Analysis of pentacyclic triterpenes by LC-MS. 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Psychology</topic><topic>LC-MS</topic><topic>Mass Spectrometry - methods</topic><topic>Other chromatographic methods</topic><topic>pentacyclic triterpenes</topic><topic>Pentacyclic Triterpenes - analysis</topic><topic>Plant Extracts - analysis</topic><topic>Plant physiology and development</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Toluene - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rhourri-Frih, B</creatorcontrib><creatorcontrib>Chaimbault, P</creatorcontrib><creatorcontrib>Claude, B</creatorcontrib><creatorcontrib>Lamy, C</creatorcontrib><creatorcontrib>André, P</creatorcontrib><creatorcontrib>Lafosse, M</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of mass spectrometry.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rhourri-Frih, B</au><au>Chaimbault, P</au><au>Claude, B</au><au>Lamy, C</au><au>André, P</au><au>Lafosse, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of pentacyclic triterpenes by LC-MS. 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Detection parameters were optimized for both the sources, and the effects of three dopants (toluene, acetone and anisole) on the detection (sensitivity and ion fingerprints in MS spectra) were studied in detail for APPI-MS.The limits of quantification were measured under selected ion monitoring conditions, in the range of 0.005-0.015 mg l⁻¹ and 0.002-0.84 mg l⁻¹ in APPI and APCI, respectively, depending on the studied pentacyclic triterpene. Overall, APPI was found more sensitive than APCI in positive ion mode, whereas APCI shows the greatest sensitivity for acidic triterpenes in negative ion mode.Following this study, the developed LC-MS method was used for the characterization of pentacyclic triterpenes in three plant extracts. High amounts of betulinic acid, betulinic aldehyde and betulinic aldehyde acetate were observed in plane bark. The main component of birch bark is betulin and extracts of okoume resin exhibit high amounts of α- and β-amyrin. Copyright © 2008 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>18946879</pmid><doi>10.1002/jms.1472</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 1076-5174 |
| ispartof | Journal of mass spectrometry., 2009, Vol.44 (1), p.71-80 |
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| subjects | Acetone - chemistry Analytical chemistry Anisoles - chemistry APCI APPI Atmospheric Pressure Biological and medical sciences Chemical constitution Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Liquid - methods Exact sciences and technology Fundamental and applied biological sciences. Psychology LC-MS Mass Spectrometry - methods Other chromatographic methods pentacyclic triterpenes Pentacyclic Triterpenes - analysis Plant Extracts - analysis Plant physiology and development Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods Toluene - chemistry |
| title | Analysis of pentacyclic triterpenes by LC-MS. A comparative study between APCI and APPI |
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