Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs
Purpose Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model. Materials and Methods Tooth and bone constructs were prepared from th...
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| Veröffentlicht in: | Journal of oral and maxillofacial surgery 2009-02, Vol.67 (2), p.335-347 |
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| creator | Abukawa, Harutsugi, DDS, PhD Zhang, Weibo, DMD, PhD Young, Conan S., PhD Asrican, Rose, MS Vacanti, Joseph P., MD Kaban, Leonard B., DMD, MD Troulis, Maria J., DDS, MSc Yelick, Pamela C., PhD |
| description | Purpose Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model. Materials and Methods Tooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses. Results Small tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament tissues, surrounded by new bone. No dental tissues formed in implants without tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using tooth-specific and bone-specific antibodies confirmed the identity of regenerated tissues. Conclusions This pilot study supports the feasibility of tissue-engineering approaches for coordinated autologous tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans. |
| doi_str_mv | 10.1016/j.joms.2008.09.002 |
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To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model. Materials and Methods Tooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses. Results Small tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament tissues, surrounded by new bone. No dental tissues formed in implants without tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using tooth-specific and bone-specific antibodies confirmed the identity of regenerated tissues. Conclusions This pilot study supports the feasibility of tissue-engineering approaches for coordinated autologous tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans.</description><identifier>ISSN: 0278-2391</identifier><identifier>EISSN: 1531-5053</identifier><identifier>DOI: 10.1016/j.joms.2008.09.002</identifier><identifier>PMID: 19138608</identifier><identifier>CODEN: JOMSDA</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Amelogenin - biosynthesis ; Animals ; Biological and medical sciences ; Bone Regeneration - physiology ; Cell Culture Techniques ; Cell Differentiation ; Cells, Cultured ; Dentistry ; Epithelial Cells - transplantation ; Feasibility Studies ; Male ; Mandible - cytology ; Mandible - surgery ; Medical sciences ; Mesenchymal Stem Cells - cytology ; Models, Animal ; Odontogenesis - physiology ; Osteoblasts - transplantation ; Otorhinolaryngology. Stomatology ; Phosphoproteins - biosynthesis ; Pilot Projects ; Regeneration - physiology ; Surgery ; Swine ; Swine, Miniature ; Tissue Engineering - methods ; Tissue Scaffolds ; Tooth - cytology ; Tooth - metabolism ; Tooth Germ - transplantation</subject><ispartof>Journal of oral and maxillofacial surgery, 2009-02, Vol.67 (2), p.335-347</ispartof><rights>American Association of Oral and Maxillofacial Surgeons</rights><rights>2009 American Association of Oral and Maxillofacial Surgeons</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-4152f98368f36b4772061a1f39f05cd4138508746ecfd2ae14879dc97df9d7993</citedby><cites>FETCH-LOGICAL-c536t-4152f98368f36b4772061a1f39f05cd4138508746ecfd2ae14879dc97df9d7993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0278239108014705$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21121210$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19138608$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abukawa, Harutsugi, DDS, PhD</creatorcontrib><creatorcontrib>Zhang, Weibo, DMD, PhD</creatorcontrib><creatorcontrib>Young, Conan S., PhD</creatorcontrib><creatorcontrib>Asrican, Rose, MS</creatorcontrib><creatorcontrib>Vacanti, Joseph P., MD</creatorcontrib><creatorcontrib>Kaban, Leonard B., DMD, MD</creatorcontrib><creatorcontrib>Troulis, Maria J., DDS, MSc</creatorcontrib><creatorcontrib>Yelick, Pamela C., PhD</creatorcontrib><title>Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs</title><title>Journal of oral and maxillofacial surgery</title><addtitle>J Oral Maxillofac Surg</addtitle><description>Purpose Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model. Materials and Methods Tooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses. Results Small tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament tissues, surrounded by new bone. No dental tissues formed in implants without tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using tooth-specific and bone-specific antibodies confirmed the identity of regenerated tissues. Conclusions This pilot study supports the feasibility of tissue-engineering approaches for coordinated autologous tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans.</description><subject>Amelogenin - biosynthesis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Regeneration - physiology</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Dentistry</subject><subject>Epithelial Cells - transplantation</subject><subject>Feasibility Studies</subject><subject>Male</subject><subject>Mandible - cytology</subject><subject>Mandible - surgery</subject><subject>Medical sciences</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Models, Animal</subject><subject>Odontogenesis - physiology</subject><subject>Osteoblasts - transplantation</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Phosphoproteins - biosynthesis</subject><subject>Pilot Projects</subject><subject>Regeneration - physiology</subject><subject>Surgery</subject><subject>Swine</subject><subject>Swine, Miniature</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds</subject><subject>Tooth - cytology</subject><subject>Tooth - metabolism</subject><subject>Tooth Germ - transplantation</subject><issn>0278-2391</issn><issn>1531-5053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkt2L1DAUxYMo7rj6D_ggfdG31nvTNh8gwjquH7Ai6OyroZPejhk7yZq0wv73psyo4IOShzzkd04O517GHiNUCCie76t9OKSKA6gKdAXA77AVtjWWLbT1XbYCLlXJa41n7EFKewDEVor77Aw11kqAWrEvn8gGn6Y428n5XfGh873bzmMXi9c0kJ1ScZ2Wh4t5CmPYhTkVG5fSTOWl3zlPFKkvNiFMX4ssLV4FT8X6l2N6yO4N3Zjo0ek-Z9dvLjfrd-XVx7fv1xdXpW1rMZUNtnzQqhZqqMW2kZKDwA6HWg_Q2r7JaVtQshFkh553hI2Surda9oPupdb1OXt29L2J4ftMaTIHlyyNY-cpRzZCKNAa8b8gh1pJFDKD_AjaGFKKNJib6A5dvDUIZqnf7M1Sv1nqN6BNrj-Lnpzc5-2B-j-SU98ZeHoCumS7cYidty795jgizwcy9-LIUS7th6NoknXkLfUu5qGYPrh_53j5l9yOzrv84ze6pbQPc_R5HAZN4gbM52VRlj0BBdjIvDw_AVFvt_Y</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Abukawa, Harutsugi, DDS, PhD</creator><creator>Zhang, Weibo, DMD, PhD</creator><creator>Young, Conan S., PhD</creator><creator>Asrican, Rose, MS</creator><creator>Vacanti, Joseph P., MD</creator><creator>Kaban, Leonard B., DMD, MD</creator><creator>Troulis, Maria J., DDS, MSc</creator><creator>Yelick, Pamela C., PhD</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090201</creationdate><title>Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs</title><author>Abukawa, Harutsugi, DDS, PhD ; Zhang, Weibo, DMD, PhD ; Young, Conan S., PhD ; Asrican, Rose, MS ; Vacanti, Joseph P., MD ; Kaban, Leonard B., DMD, MD ; Troulis, Maria J., DDS, MSc ; Yelick, Pamela C., PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-4152f98368f36b4772061a1f39f05cd4138508746ecfd2ae14879dc97df9d7993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amelogenin - biosynthesis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Regeneration - physiology</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Dentistry</topic><topic>Epithelial Cells - transplantation</topic><topic>Feasibility Studies</topic><topic>Male</topic><topic>Mandible - cytology</topic><topic>Mandible - surgery</topic><topic>Medical sciences</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Models, Animal</topic><topic>Odontogenesis - physiology</topic><topic>Osteoblasts - transplantation</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Phosphoproteins - biosynthesis</topic><topic>Pilot Projects</topic><topic>Regeneration - physiology</topic><topic>Surgery</topic><topic>Swine</topic><topic>Swine, Miniature</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds</topic><topic>Tooth - cytology</topic><topic>Tooth - metabolism</topic><topic>Tooth Germ - transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abukawa, Harutsugi, DDS, PhD</creatorcontrib><creatorcontrib>Zhang, Weibo, DMD, PhD</creatorcontrib><creatorcontrib>Young, Conan S., PhD</creatorcontrib><creatorcontrib>Asrican, Rose, MS</creatorcontrib><creatorcontrib>Vacanti, Joseph P., MD</creatorcontrib><creatorcontrib>Kaban, Leonard B., DMD, MD</creatorcontrib><creatorcontrib>Troulis, Maria J., DDS, MSc</creatorcontrib><creatorcontrib>Yelick, Pamela C., PhD</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of oral and maxillofacial surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abukawa, Harutsugi, DDS, PhD</au><au>Zhang, Weibo, DMD, PhD</au><au>Young, Conan S., PhD</au><au>Asrican, Rose, MS</au><au>Vacanti, Joseph P., MD</au><au>Kaban, Leonard B., DMD, MD</au><au>Troulis, Maria J., DDS, MSc</au><au>Yelick, Pamela C., PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs</atitle><jtitle>Journal of oral and maxillofacial surgery</jtitle><addtitle>J Oral Maxillofac Surg</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>67</volume><issue>2</issue><spage>335</spage><epage>347</epage><pages>335-347</pages><issn>0278-2391</issn><eissn>1531-5053</eissn><coden>JOMSDA</coden><abstract>Purpose Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model. Materials and Methods Tooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses. Results Small tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament tissues, surrounded by new bone. No dental tissues formed in implants without tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using tooth-specific and bone-specific antibodies confirmed the identity of regenerated tissues. Conclusions This pilot study supports the feasibility of tissue-engineering approaches for coordinated autologous tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>19138608</pmid><doi>10.1016/j.joms.2008.09.002</doi><tpages>13</tpages></addata></record> |
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| subjects | Amelogenin - biosynthesis Animals Biological and medical sciences Bone Regeneration - physiology Cell Culture Techniques Cell Differentiation Cells, Cultured Dentistry Epithelial Cells - transplantation Feasibility Studies Male Mandible - cytology Mandible - surgery Medical sciences Mesenchymal Stem Cells - cytology Models, Animal Odontogenesis - physiology Osteoblasts - transplantation Otorhinolaryngology. Stomatology Phosphoproteins - biosynthesis Pilot Projects Regeneration - physiology Surgery Swine Swine, Miniature Tissue Engineering - methods Tissue Scaffolds Tooth - cytology Tooth - metabolism Tooth Germ - transplantation |
| title | Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs |
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