Towards a pragmatic strategy for regenerating infarcted myocardium with glandular stem cells

We have recently reported that the in vitro differentiation of human glandular stem cells into cardiac-like cells can be enhanced by co-culture with small myocardial biopsies. These results suggest that implantation of such cells directly into infarcted myocardium may facilitate the regeneration of...

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Veröffentlicht in:Annals of anatomy 2009, Vol.191 (1), p.51-61
Hauptverfasser: Maass, Antje, Kajahn, Jennifer, Guerleyik, Emel, Guldner, Norbert W., Rapoport, Daniel H., Kruse, Charli
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container_end_page 61
container_issue 1
container_start_page 51
container_title Annals of anatomy
container_volume 191
creator Maass, Antje
Kajahn, Jennifer
Guerleyik, Emel
Guldner, Norbert W.
Rapoport, Daniel H.
Kruse, Charli
description We have recently reported that the in vitro differentiation of human glandular stem cells into cardiac-like cells can be enhanced by co-culture with small myocardial biopsies. These results suggest that implantation of such cells directly into infarcted myocardium may facilitate the regeneration of the heart. As a preliminary to testing this approach in a goat model, pilot in vitro tests for these experiments have been performed and are presented here. Stem cells, isolated from the glandula submandibularis of Boer goats (SuSCs), have been co-cultured either directly or indirectly with heart biopsies from various species (Boer goat, rattus norwegicus, human) or heart conditioned medium for 48 h. We found a substantial increase in the number of cells expressing heart-specific marker proteins (Troponin I, Troponin T, sarcomeric myosin) regardless of the source organism of the heart biopsy. The proliferation of SuSCs also increased significantly under co-culture conditions. To benefit from these results in vivo, the stem cells must be delivered to the infarcted region in the heart and held securely in place over lengthy periods of time. Therefore, we repeated the co-culture experiments with SuSCs grown on biodegradable Vicryl ®-meshes. The cells demonstrated good proliferation on the meshes and likewise, the expression of heart-specific marker proteins could be enhanced through co-culture with heart biopsies.
doi_str_mv 10.1016/j.aanat.2008.09.006
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subjects Animals
Application
Cardiac-like cells
Cell Differentiation
Cell Division
Coculture Techniques - methods
Culture Media, Conditioned
Disease Models, Animal
Glandular tissue
Goats
Humans
Male
Myocardial Infarction - physiopathology
Myocardial Infarction - surgery
Pilot Projects
Rats
Rats, Sprague-Dawley
Regeneration - physiology
Salivary Glands - cytology
Salivary Glands - physiology
Species Specificity
Stem Cell Transplantation - methods
Stem cells
Stem Cells - cytology
Stem Cells - physiology
Submandibular Gland - cytology
Submandibular Gland - physiology
title Towards a pragmatic strategy for regenerating infarcted myocardium with glandular stem cells
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