Towards a pragmatic strategy for regenerating infarcted myocardium with glandular stem cells
We have recently reported that the in vitro differentiation of human glandular stem cells into cardiac-like cells can be enhanced by co-culture with small myocardial biopsies. These results suggest that implantation of such cells directly into infarcted myocardium may facilitate the regeneration of...
Gespeichert in:
Veröffentlicht in: | Annals of anatomy 2009, Vol.191 (1), p.51-61 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 61 |
---|---|
container_issue | 1 |
container_start_page | 51 |
container_title | Annals of anatomy |
container_volume | 191 |
creator | Maass, Antje Kajahn, Jennifer Guerleyik, Emel Guldner, Norbert W. Rapoport, Daniel H. Kruse, Charli |
description | We have recently reported that the
in vitro differentiation of human glandular stem cells into cardiac-like cells can be enhanced by co-culture with small myocardial biopsies. These results suggest that implantation of such cells directly into infarcted myocardium may facilitate the regeneration of the heart. As a preliminary to testing this approach in a goat model, pilot
in vitro tests for these experiments have been performed and are presented here. Stem cells, isolated from the glandula submandibularis of Boer goats (SuSCs), have been co-cultured either directly or indirectly with heart biopsies from various species (Boer goat, rattus norwegicus, human) or heart conditioned medium for 48
h. We found a substantial increase in the number of cells expressing heart-specific marker proteins (Troponin I, Troponin T, sarcomeric myosin) regardless of the source organism of the heart biopsy. The proliferation of SuSCs also increased significantly under co-culture conditions. To benefit from these results
in vivo, the stem cells must be delivered to the infarcted region in the heart and held securely in place over lengthy periods of time. Therefore, we repeated the co-culture experiments with SuSCs grown on biodegradable Vicryl
®-meshes. The cells demonstrated good proliferation on the meshes and likewise, the expression of heart-specific marker proteins could be enhanced through co-culture with heart biopsies. |
doi_str_mv | 10.1016/j.aanat.2008.09.006 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66804752</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0940960208001490</els_id><sourcerecordid>66804752</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-e3933344dbb7c9db05d4a92195f0905a600226a28b2294daf8f7039eb59b96623</originalsourceid><addsrcrecordid>eNp9kE9r3DAQxUVISbZpP0Eh6NSb3bFky9YhhxCatBDoJb0VxFgau1r8ZyPJCfvt680u9JbTwPDem3k_xr4UkBdQqG_bHHHClAuAJgedA6gztilU0WRQgjhnG9AlZFqBuGQfY9wCSFWp8oJdFhpqKGuxYX-e5lcMLnLku4D9iMlbHlPARP2ed3PggXqaaF34qed-6jDYRI6P-9muRr-M_NWnv7wfcHLLgGF108gtDUP8xD50OET6fJpX7Pf996e7H9njr4efd7ePmZVVnTKSWkpZlq5ta6tdC5UrUYtCVx1oqFABCKFQNK0QunTYNV0NUlNb6VYrJeQV-3rM3YX5eaGYzOjj4QOcaF6iUapZ21YHoTwKbZhjDNSZXfAjhr0pwBygmq15g2oOUA1os0JdXden-KUdyf33nCiugpujgNaSL56CidbTZMn5QDYZN_t3D_wDMGGJ9A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66804752</pqid></control><display><type>article</type><title>Towards a pragmatic strategy for regenerating infarcted myocardium with glandular stem cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Maass, Antje ; Kajahn, Jennifer ; Guerleyik, Emel ; Guldner, Norbert W. ; Rapoport, Daniel H. ; Kruse, Charli</creator><creatorcontrib>Maass, Antje ; Kajahn, Jennifer ; Guerleyik, Emel ; Guldner, Norbert W. ; Rapoport, Daniel H. ; Kruse, Charli</creatorcontrib><description>We have recently reported that the
in vitro differentiation of human glandular stem cells into cardiac-like cells can be enhanced by co-culture with small myocardial biopsies. These results suggest that implantation of such cells directly into infarcted myocardium may facilitate the regeneration of the heart. As a preliminary to testing this approach in a goat model, pilot
in vitro tests for these experiments have been performed and are presented here. Stem cells, isolated from the glandula submandibularis of Boer goats (SuSCs), have been co-cultured either directly or indirectly with heart biopsies from various species (Boer goat, rattus norwegicus, human) or heart conditioned medium for 48
h. We found a substantial increase in the number of cells expressing heart-specific marker proteins (Troponin I, Troponin T, sarcomeric myosin) regardless of the source organism of the heart biopsy. The proliferation of SuSCs also increased significantly under co-culture conditions. To benefit from these results
in vivo, the stem cells must be delivered to the infarcted region in the heart and held securely in place over lengthy periods of time. Therefore, we repeated the co-culture experiments with SuSCs grown on biodegradable Vicryl
®-meshes. The cells demonstrated good proliferation on the meshes and likewise, the expression of heart-specific marker proteins could be enhanced through co-culture with heart biopsies.</description><identifier>ISSN: 0940-9602</identifier><identifier>EISSN: 1618-0402</identifier><identifier>DOI: 10.1016/j.aanat.2008.09.006</identifier><identifier>PMID: 19070472</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Animals ; Application ; Cardiac-like cells ; Cell Differentiation ; Cell Division ; Coculture Techniques - methods ; Culture Media, Conditioned ; Disease Models, Animal ; Glandular tissue ; Goats ; Humans ; Male ; Myocardial Infarction - physiopathology ; Myocardial Infarction - surgery ; Pilot Projects ; Rats ; Rats, Sprague-Dawley ; Regeneration - physiology ; Salivary Glands - cytology ; Salivary Glands - physiology ; Species Specificity ; Stem Cell Transplantation - methods ; Stem cells ; Stem Cells - cytology ; Stem Cells - physiology ; Submandibular Gland - cytology ; Submandibular Gland - physiology</subject><ispartof>Annals of anatomy, 2009, Vol.191 (1), p.51-61</ispartof><rights>2008 Elsevier GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-e3933344dbb7c9db05d4a92195f0905a600226a28b2294daf8f7039eb59b96623</citedby><cites>FETCH-LOGICAL-c357t-e3933344dbb7c9db05d4a92195f0905a600226a28b2294daf8f7039eb59b96623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aanat.2008.09.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19070472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maass, Antje</creatorcontrib><creatorcontrib>Kajahn, Jennifer</creatorcontrib><creatorcontrib>Guerleyik, Emel</creatorcontrib><creatorcontrib>Guldner, Norbert W.</creatorcontrib><creatorcontrib>Rapoport, Daniel H.</creatorcontrib><creatorcontrib>Kruse, Charli</creatorcontrib><title>Towards a pragmatic strategy for regenerating infarcted myocardium with glandular stem cells</title><title>Annals of anatomy</title><addtitle>Ann Anat</addtitle><description>We have recently reported that the
in vitro differentiation of human glandular stem cells into cardiac-like cells can be enhanced by co-culture with small myocardial biopsies. These results suggest that implantation of such cells directly into infarcted myocardium may facilitate the regeneration of the heart. As a preliminary to testing this approach in a goat model, pilot
in vitro tests for these experiments have been performed and are presented here. Stem cells, isolated from the glandula submandibularis of Boer goats (SuSCs), have been co-cultured either directly or indirectly with heart biopsies from various species (Boer goat, rattus norwegicus, human) or heart conditioned medium for 48
h. We found a substantial increase in the number of cells expressing heart-specific marker proteins (Troponin I, Troponin T, sarcomeric myosin) regardless of the source organism of the heart biopsy. The proliferation of SuSCs also increased significantly under co-culture conditions. To benefit from these results
in vivo, the stem cells must be delivered to the infarcted region in the heart and held securely in place over lengthy periods of time. Therefore, we repeated the co-culture experiments with SuSCs grown on biodegradable Vicryl
®-meshes. The cells demonstrated good proliferation on the meshes and likewise, the expression of heart-specific marker proteins could be enhanced through co-culture with heart biopsies.</description><subject>Animals</subject><subject>Application</subject><subject>Cardiac-like cells</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>Coculture Techniques - methods</subject><subject>Culture Media, Conditioned</subject><subject>Disease Models, Animal</subject><subject>Glandular tissue</subject><subject>Goats</subject><subject>Humans</subject><subject>Male</subject><subject>Myocardial Infarction - physiopathology</subject><subject>Myocardial Infarction - surgery</subject><subject>Pilot Projects</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regeneration - physiology</subject><subject>Salivary Glands - cytology</subject><subject>Salivary Glands - physiology</subject><subject>Species Specificity</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - physiology</subject><subject>Submandibular Gland - cytology</subject><subject>Submandibular Gland - physiology</subject><issn>0940-9602</issn><issn>1618-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9r3DAQxUVISbZpP0Eh6NSb3bFky9YhhxCatBDoJb0VxFgau1r8ZyPJCfvt680u9JbTwPDem3k_xr4UkBdQqG_bHHHClAuAJgedA6gztilU0WRQgjhnG9AlZFqBuGQfY9wCSFWp8oJdFhpqKGuxYX-e5lcMLnLku4D9iMlbHlPARP2ed3PggXqaaF34qed-6jDYRI6P-9muRr-M_NWnv7wfcHLLgGF108gtDUP8xD50OET6fJpX7Pf996e7H9njr4efd7ePmZVVnTKSWkpZlq5ta6tdC5UrUYtCVx1oqFABCKFQNK0QunTYNV0NUlNb6VYrJeQV-3rM3YX5eaGYzOjj4QOcaF6iUapZ21YHoTwKbZhjDNSZXfAjhr0pwBygmq15g2oOUA1os0JdXden-KUdyf33nCiugpujgNaSL56CidbTZMn5QDYZN_t3D_wDMGGJ9A</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Maass, Antje</creator><creator>Kajahn, Jennifer</creator><creator>Guerleyik, Emel</creator><creator>Guldner, Norbert W.</creator><creator>Rapoport, Daniel H.</creator><creator>Kruse, Charli</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2009</creationdate><title>Towards a pragmatic strategy for regenerating infarcted myocardium with glandular stem cells</title><author>Maass, Antje ; Kajahn, Jennifer ; Guerleyik, Emel ; Guldner, Norbert W. ; Rapoport, Daniel H. ; Kruse, Charli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-e3933344dbb7c9db05d4a92195f0905a600226a28b2294daf8f7039eb59b96623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Application</topic><topic>Cardiac-like cells</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>Coculture Techniques - methods</topic><topic>Culture Media, Conditioned</topic><topic>Disease Models, Animal</topic><topic>Glandular tissue</topic><topic>Goats</topic><topic>Humans</topic><topic>Male</topic><topic>Myocardial Infarction - physiopathology</topic><topic>Myocardial Infarction - surgery</topic><topic>Pilot Projects</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regeneration - physiology</topic><topic>Salivary Glands - cytology</topic><topic>Salivary Glands - physiology</topic><topic>Species Specificity</topic><topic>Stem Cell Transplantation - methods</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - physiology</topic><topic>Submandibular Gland - cytology</topic><topic>Submandibular Gland - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maass, Antje</creatorcontrib><creatorcontrib>Kajahn, Jennifer</creatorcontrib><creatorcontrib>Guerleyik, Emel</creatorcontrib><creatorcontrib>Guldner, Norbert W.</creatorcontrib><creatorcontrib>Rapoport, Daniel H.</creatorcontrib><creatorcontrib>Kruse, Charli</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of anatomy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maass, Antje</au><au>Kajahn, Jennifer</au><au>Guerleyik, Emel</au><au>Guldner, Norbert W.</au><au>Rapoport, Daniel H.</au><au>Kruse, Charli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Towards a pragmatic strategy for regenerating infarcted myocardium with glandular stem cells</atitle><jtitle>Annals of anatomy</jtitle><addtitle>Ann Anat</addtitle><date>2009</date><risdate>2009</risdate><volume>191</volume><issue>1</issue><spage>51</spage><epage>61</epage><pages>51-61</pages><issn>0940-9602</issn><eissn>1618-0402</eissn><abstract>We have recently reported that the
in vitro differentiation of human glandular stem cells into cardiac-like cells can be enhanced by co-culture with small myocardial biopsies. These results suggest that implantation of such cells directly into infarcted myocardium may facilitate the regeneration of the heart. As a preliminary to testing this approach in a goat model, pilot
in vitro tests for these experiments have been performed and are presented here. Stem cells, isolated from the glandula submandibularis of Boer goats (SuSCs), have been co-cultured either directly or indirectly with heart biopsies from various species (Boer goat, rattus norwegicus, human) or heart conditioned medium for 48
h. We found a substantial increase in the number of cells expressing heart-specific marker proteins (Troponin I, Troponin T, sarcomeric myosin) regardless of the source organism of the heart biopsy. The proliferation of SuSCs also increased significantly under co-culture conditions. To benefit from these results
in vivo, the stem cells must be delivered to the infarcted region in the heart and held securely in place over lengthy periods of time. Therefore, we repeated the co-culture experiments with SuSCs grown on biodegradable Vicryl
®-meshes. The cells demonstrated good proliferation on the meshes and likewise, the expression of heart-specific marker proteins could be enhanced through co-culture with heart biopsies.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>19070472</pmid><doi>10.1016/j.aanat.2008.09.006</doi><tpages>11</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0940-9602 |
ispartof | Annals of anatomy, 2009, Vol.191 (1), p.51-61 |
issn | 0940-9602 1618-0402 |
language | eng |
recordid | cdi_proquest_miscellaneous_66804752 |
source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | Animals Application Cardiac-like cells Cell Differentiation Cell Division Coculture Techniques - methods Culture Media, Conditioned Disease Models, Animal Glandular tissue Goats Humans Male Myocardial Infarction - physiopathology Myocardial Infarction - surgery Pilot Projects Rats Rats, Sprague-Dawley Regeneration - physiology Salivary Glands - cytology Salivary Glands - physiology Species Specificity Stem Cell Transplantation - methods Stem cells Stem Cells - cytology Stem Cells - physiology Submandibular Gland - cytology Submandibular Gland - physiology |
title | Towards a pragmatic strategy for regenerating infarcted myocardium with glandular stem cells |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T17%3A45%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Towards%20a%20pragmatic%20strategy%20for%20regenerating%20infarcted%20myocardium%20with%20glandular%20stem%20cells&rft.jtitle=Annals%20of%20anatomy&rft.au=Maass,%20Antje&rft.date=2009&rft.volume=191&rft.issue=1&rft.spage=51&rft.epage=61&rft.pages=51-61&rft.issn=0940-9602&rft.eissn=1618-0402&rft_id=info:doi/10.1016/j.aanat.2008.09.006&rft_dat=%3Cproquest_cross%3E66804752%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=66804752&rft_id=info:pmid/19070472&rft_els_id=S0940960208001490&rfr_iscdi=true |