Functional characterisation of Fab′-fragments self-assembled onto hydrophilic gold surfaces
Antibody Fab′-fragments have been immobilised on hydrophilic gold by direct self-assembly, and embedded in a matrix of non-ionic hydrophilic polymers, tris(hydroxymethyl)methylacrylamide, carrying lipoate terminal linking groups. Different polymers were synthesised, and co-adsorbed or post-adsorbed...
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| Veröffentlicht in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2009-02, Vol.68 (2), p.193-199 |
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| container_title | Colloids and surfaces, B, Biointerfaces |
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| creator | Albers, Willem M. Auer, Sanna Helle, Hannu Munter, Tony Vikholm-Lundin, Inger |
| description | Antibody Fab′-fragments have been immobilised on hydrophilic gold by direct self-assembly, and embedded in a matrix of non-ionic hydrophilic polymers, tris(hydroxymethyl)methylacrylamide, carrying lipoate terminal linking groups. Different polymers were synthesised, and co-adsorbed or post-adsorbed between the antibody fragments in order to optimise the antigen binding. Various factors were investigated that influence the activity of the immobilised Fab′-fragments for binding of the antigen, human IgG. The Fab′-fragments were immobilised in dense layers close to monolayer coverage, and the stoichiometric efficiency of immobilisation was up to 30%, with the human IgG also approaching monolayer coverage. The cleaning of the gold surface was a crucial factor in preservation of activity. Besides the usual treatment in hot ammonia/peroxide solution, hot DMSO appeared to be highly effective as a cleaning agent. |
| doi_str_mv | 10.1016/j.colsurfb.2008.10.001 |
| format | Article |
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Besides the usual treatment in hot ammonia/peroxide solution, hot DMSO appeared to be highly effective as a cleaning agent.</description><subject>Adsorption</subject><subject>Antibody Fab′-fragment</subject><subject>Gold - chemistry</subject><subject>Heterogeneous binding</subject><subject>Humans</subject><subject>Immobilisation</subject><subject>Immobilized Proteins - immunology</subject><subject>Immunoassay</subject><subject>Immunoglobulin Fab Fragments - immunology</subject><subject>Immunoglobulin G - immunology</subject><subject>Kinetics</subject><subject>Polymers - chemistry</subject><subject>Reference Standards</subject><subject>Surface Plasmon Resonance</subject><subject>Surface Properties</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1qGzEUhUVJqR23r2Bmld04-pmRrF2DiduCoZt2WYRGc2XLaEauNBPILs-UR-qTVIMdsvTqwuG75_4chJYErwgm_P64MsGnMdpmRTFeZ3GFMfmA5mQtWFkxLm7QHEsqSiF4PUO3KR0xxrQi4hOaEYkp5ayaoz_bsTeDC732hTnoqM0A0SU9SUWwxVY3_15eSxv1voN-SEUCb0udEnSNh7YI_RCKw3Mbw-ngvDPFPvi2mBbTBtJn9NFqn-DLpS7Q7-3jr833cvfz24_Nw640TIqhtMa2otFSNqKilQZiBMVgbV7R6lpIwiRjDdVg1rxpuZSS1qJmleA0X2QkW6C7s-8phr8jpEF1LhnwXvcQxqQ4X-OK1NfByU9k-wzyM2hiSCmCVafoOh2fFcFqSkAd1VsCakpg0nMCuXF5mTA2HbTvbZeXZ-DrGYD8kCcHUSXjoDfQughmUG1w12b8B_jGnQo</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Albers, Willem M.</creator><creator>Auer, Sanna</creator><creator>Helle, Hannu</creator><creator>Munter, Tony</creator><creator>Vikholm-Lundin, Inger</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090201</creationdate><title>Functional characterisation of Fab′-fragments self-assembled onto hydrophilic gold surfaces</title><author>Albers, Willem M. ; Auer, Sanna ; Helle, Hannu ; Munter, Tony ; Vikholm-Lundin, Inger</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-fcfd7ba99b7424ae1c720eff263fa57913933b2aec86bd6999257534762024c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adsorption</topic><topic>Antibody Fab′-fragment</topic><topic>Gold - chemistry</topic><topic>Heterogeneous binding</topic><topic>Humans</topic><topic>Immobilisation</topic><topic>Immobilized Proteins - immunology</topic><topic>Immunoassay</topic><topic>Immunoglobulin Fab Fragments - immunology</topic><topic>Immunoglobulin G - immunology</topic><topic>Kinetics</topic><topic>Polymers - chemistry</topic><topic>Reference Standards</topic><topic>Surface Plasmon Resonance</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Albers, Willem M.</creatorcontrib><creatorcontrib>Auer, Sanna</creatorcontrib><creatorcontrib>Helle, Hannu</creatorcontrib><creatorcontrib>Munter, Tony</creatorcontrib><creatorcontrib>Vikholm-Lundin, Inger</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Albers, Willem M.</au><au>Auer, Sanna</au><au>Helle, Hannu</au><au>Munter, Tony</au><au>Vikholm-Lundin, Inger</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional characterisation of Fab′-fragments self-assembled onto hydrophilic gold surfaces</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>68</volume><issue>2</issue><spage>193</spage><epage>199</epage><pages>193-199</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>Antibody Fab′-fragments have been immobilised on hydrophilic gold by direct self-assembly, and embedded in a matrix of non-ionic hydrophilic polymers, tris(hydroxymethyl)methylacrylamide, carrying lipoate terminal linking groups. 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| fulltext | fulltext |
| identifier | ISSN: 0927-7765 |
| ispartof | Colloids and surfaces, B, Biointerfaces, 2009-02, Vol.68 (2), p.193-199 |
| issn | 0927-7765 1873-4367 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66804159 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adsorption Antibody Fab′-fragment Gold - chemistry Heterogeneous binding Humans Immobilisation Immobilized Proteins - immunology Immunoassay Immunoglobulin Fab Fragments - immunology Immunoglobulin G - immunology Kinetics Polymers - chemistry Reference Standards Surface Plasmon Resonance Surface Properties |
| title | Functional characterisation of Fab′-fragments self-assembled onto hydrophilic gold surfaces |
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