Bombyx mori nucleopolyhedrovirus ORF51 encodes a budded virus envelope associated protein
Bombyx mori nucleopolyhedrovirus (BmNPV) ORF 51 (Bm51) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm51 was characterized. Transcripts of Bm51 were detected from 4.5 through 72 hour post infection (h p.i.) by RT-PCR. The corresponding protein was detected...
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| Veröffentlicht in: | Virus genes 2009-02, Vol.38 (1), p.171-177 |
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| creator | Tian, C.-H Tang, X.-D Xu, H.-J Ge, J.-Q Miao, Y.-G Zhang, C.-X |
| description | Bombyx mori nucleopolyhedrovirus (BmNPV) ORF 51 (Bm51) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm51 was characterized. Transcripts of Bm51 were detected from 4.5 through 72 hour post infection (h p.i.) by RT-PCR. The corresponding protein was detected from 6 to 72 h p.i. in BmNPV-infected BmN cells by western blot analysis using a polyclonal antibody against Bm51. Western blot assay of occlusion-derived virus and budded virus (BV) preparations revealed that Bm51 encodes a 23-kDa structural protein that is associated with BV and is located in the envelope fraction of budded virions. The protein was temporarily called BV-E23. In addition immunofluorescence microscopy demonstrated that the protein was present within the cytoplasm and nuclei in virus-infected cells. In conclusion, the available data suggest that Bm51 is a functional ORF of BmNPV and encodes a protein expressed in the early stage of the infection cycle that is associated with the BV envelope. |
| doi_str_mv | 10.1007/s11262-008-0312-3 |
| format | Article |
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In this study, Bm51 was characterized. Transcripts of Bm51 were detected from 4.5 through 72 hour post infection (h p.i.) by RT-PCR. The corresponding protein was detected from 6 to 72 h p.i. in BmNPV-infected BmN cells by western blot analysis using a polyclonal antibody against Bm51. Western blot assay of occlusion-derived virus and budded virus (BV) preparations revealed that Bm51 encodes a 23-kDa structural protein that is associated with BV and is located in the envelope fraction of budded virions. The protein was temporarily called BV-E23. In addition immunofluorescence microscopy demonstrated that the protein was present within the cytoplasm and nuclei in virus-infected cells. In conclusion, the available data suggest that Bm51 is a functional ORF of BmNPV and encodes a protein expressed in the early stage of the infection cycle that is associated with the BV envelope.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-008-0312-3</identifier><identifier>PMID: 19085095</identifier><language>eng</language><publisher>New York: Boston : Springer US</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Western ; Bombyx - virology ; Bombyx mori NPV ; Cell Line ; Cell Nucleus ; Cytoplasm - chemistry ; Gene Expression Profiling ; Genes, Viral ; Lepidoptera ; Medical Microbiology ; Molecular Sequence Data ; Molecular Weight ; Nuclear polyhedrosis virus ; Nucleopolyhedrovirus - genetics ; Plant Sciences ; Sequence Alignment ; Time Factors ; Viral Envelope Proteins - biosynthesis ; Viral Envelope Proteins - chemistry ; Viral Envelope Proteins - genetics ; Virion - chemistry ; Virology</subject><ispartof>Virus genes, 2009-02, Vol.38 (1), p.171-177</ispartof><rights>Springer Science+Business Media, LLC 2008</rights><rights>Springer Science+Business Media, LLC 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-dda022624d9e3beef5f25bc94b7c558d4e8fb2dd3a7e5e88c7125026098b532c3</citedby><cites>FETCH-LOGICAL-c424t-dda022624d9e3beef5f25bc94b7c558d4e8fb2dd3a7e5e88c7125026098b532c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11262-008-0312-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11262-008-0312-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19085095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian, C.-H</creatorcontrib><creatorcontrib>Tang, X.-D</creatorcontrib><creatorcontrib>Xu, H.-J</creatorcontrib><creatorcontrib>Ge, J.-Q</creatorcontrib><creatorcontrib>Miao, Y.-G</creatorcontrib><creatorcontrib>Zhang, C.-X</creatorcontrib><title>Bombyx mori nucleopolyhedrovirus ORF51 encodes a budded virus envelope associated protein</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><addtitle>Virus Genes</addtitle><description>Bombyx mori nucleopolyhedrovirus (BmNPV) ORF 51 (Bm51) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm51 was characterized. Transcripts of Bm51 were detected from 4.5 through 72 hour post infection (h p.i.) by RT-PCR. The corresponding protein was detected from 6 to 72 h p.i. in BmNPV-infected BmN cells by western blot analysis using a polyclonal antibody against Bm51. Western blot assay of occlusion-derived virus and budded virus (BV) preparations revealed that Bm51 encodes a 23-kDa structural protein that is associated with BV and is located in the envelope fraction of budded virions. The protein was temporarily called BV-E23. In addition immunofluorescence microscopy demonstrated that the protein was present within the cytoplasm and nuclei in virus-infected cells. In conclusion, the available data suggest that Bm51 is a functional ORF of BmNPV and encodes a protein expressed in the early stage of the infection cycle that is associated with the BV envelope.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Western</subject><subject>Bombyx - virology</subject><subject>Bombyx mori NPV</subject><subject>Cell Line</subject><subject>Cell Nucleus</subject><subject>Cytoplasm - chemistry</subject><subject>Gene Expression Profiling</subject><subject>Genes, Viral</subject><subject>Lepidoptera</subject><subject>Medical Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Nuclear polyhedrosis virus</subject><subject>Nucleopolyhedrovirus - genetics</subject><subject>Plant Sciences</subject><subject>Sequence Alignment</subject><subject>Time Factors</subject><subject>Viral Envelope Proteins - biosynthesis</subject><subject>Viral Envelope Proteins - chemistry</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Virion - chemistry</subject><subject>Virology</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk9P3DAQxa2qVdlCPwCXNuqht5TxOE7sY4ugrYSExB-JnizHnkBQEm_tDWK_PUZZCamHcvLh_d6MZ94wdsjhGwdojhLnWGMJoEoQHEvxhq24bLDUurp5y1agEUola73HPqR0DxlUWL1ne1yDkqDliv35EcZ2-1iMIfbFNLuBwjoM2zvyMTz0cU7F-cWp5AVNLnhKhS3a2XvyxSLS9EBDWFNhUwqut5usrGPYUD8dsHedHRJ93L377Pr05Or4V3l2_vP38fez0lVYbUrvLWCeovKaREvUyQ5l63TVNk5K5StSXYveC9uQJKVcw1EC1qBVKwU6sc--LnVz378zpY0Z--RoGOxEYU6mrhUIAfgqiJBXkn-SwS__gPdhjlMewiCKukbAJkN8gVwMKUXqzDr2o41bw8E8p2OWdExeunlOx4js-bQrPLcj-RfHLo4M4AKkLE23FF86_6_q58XU2WDsbeyTub5E4ALyLWhdc_EEvi2jdA</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Tian, C.-H</creator><creator>Tang, X.-D</creator><creator>Xu, H.-J</creator><creator>Ge, J.-Q</creator><creator>Miao, Y.-G</creator><creator>Zhang, C.-X</creator><general>Boston : Springer US</general><general>Springer US</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20090201</creationdate><title>Bombyx mori nucleopolyhedrovirus ORF51 encodes a budded virus envelope associated protein</title><author>Tian, C.-H ; Tang, X.-D ; Xu, H.-J ; Ge, J.-Q ; Miao, Y.-G ; Zhang, C.-X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-dda022624d9e3beef5f25bc94b7c558d4e8fb2dd3a7e5e88c7125026098b532c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blotting, Western</topic><topic>Bombyx - virology</topic><topic>Bombyx mori NPV</topic><topic>Cell Line</topic><topic>Cell Nucleus</topic><topic>Cytoplasm - chemistry</topic><topic>Gene Expression Profiling</topic><topic>Genes, Viral</topic><topic>Lepidoptera</topic><topic>Medical Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Nuclear polyhedrosis virus</topic><topic>Nucleopolyhedrovirus - genetics</topic><topic>Plant Sciences</topic><topic>Sequence Alignment</topic><topic>Time Factors</topic><topic>Viral Envelope Proteins - biosynthesis</topic><topic>Viral Envelope Proteins - chemistry</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Virion - chemistry</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian, C.-H</creatorcontrib><creatorcontrib>Tang, X.-D</creatorcontrib><creatorcontrib>Xu, H.-J</creatorcontrib><creatorcontrib>Ge, J.-Q</creatorcontrib><creatorcontrib>Miao, Y.-G</creatorcontrib><creatorcontrib>Zhang, C.-X</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian, C.-H</au><au>Tang, X.-D</au><au>Xu, H.-J</au><au>Ge, J.-Q</au><au>Miao, Y.-G</au><au>Zhang, C.-X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bombyx mori nucleopolyhedrovirus ORF51 encodes a budded virus envelope associated protein</atitle><jtitle>Virus genes</jtitle><stitle>Virus Genes</stitle><addtitle>Virus Genes</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>38</volume><issue>1</issue><spage>171</spage><epage>177</epage><pages>171-177</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>Bombyx mori nucleopolyhedrovirus (BmNPV) ORF 51 (Bm51) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm51 was characterized. Transcripts of Bm51 were detected from 4.5 through 72 hour post infection (h p.i.) by RT-PCR. The corresponding protein was detected from 6 to 72 h p.i. in BmNPV-infected BmN cells by western blot analysis using a polyclonal antibody against Bm51. Western blot assay of occlusion-derived virus and budded virus (BV) preparations revealed that Bm51 encodes a 23-kDa structural protein that is associated with BV and is located in the envelope fraction of budded virions. The protein was temporarily called BV-E23. In addition immunofluorescence microscopy demonstrated that the protein was present within the cytoplasm and nuclei in virus-infected cells. In conclusion, the available data suggest that Bm51 is a functional ORF of BmNPV and encodes a protein expressed in the early stage of the infection cycle that is associated with the BV envelope.</abstract><cop>New York</cop><pub>Boston : Springer US</pub><pmid>19085095</pmid><doi>10.1007/s11262-008-0312-3</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Base Sequence Biomedical and Life Sciences Biomedicine Blotting, Western Bombyx - virology Bombyx mori NPV Cell Line Cell Nucleus Cytoplasm - chemistry Gene Expression Profiling Genes, Viral Lepidoptera Medical Microbiology Molecular Sequence Data Molecular Weight Nuclear polyhedrosis virus Nucleopolyhedrovirus - genetics Plant Sciences Sequence Alignment Time Factors Viral Envelope Proteins - biosynthesis Viral Envelope Proteins - chemistry Viral Envelope Proteins - genetics Virion - chemistry Virology |
| title | Bombyx mori nucleopolyhedrovirus ORF51 encodes a budded virus envelope associated protein |
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