Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1
We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA (a structur...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-08, Vol.68 (8), p.1663-1671 |
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| creator | Aso, Y. (Kyushu Univ., Fukuoka (Japan)) Sashihara, T Nagao, J Kanemasa, Y Koga, H Hashimoto, T Higuchi, T Adachi, A Nomiyama, H Ishizaki, A Nakayama, J Sonomoto, K |
| description | We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA (a structural gene), -M, -T, -F, -E, -G, and two open reading frames, ORF1 and ORF7. NukM and NukT were predicted to be involved in post-translational modification and secretion of nukacin ISK-1 respectively. NukF, -E, and -G were predicted to form a membrane complex which contributes to self-protection from nukacin ISK-1. Transcriptional analyses revealed that nukM through ORF7 comprises an operon, and that ORF1 is transcribed independently from downstream of nukA. The transcriptional levels of the nukA and nukM genes were enhanced by osmotic stress. The expression level of the nukA transcript was scarcely enhanced by nukacin ISK-1, suggesting that expression is not under the control of the autoregulatory circuit. |
| doi_str_mv | 10.1271/bbb.68.1663 |
| format | Article |
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(Kyushu Univ., Fukuoka (Japan)) ; Sashihara, T ; Nagao, J ; Kanemasa, Y ; Koga, H ; Hashimoto, T ; Higuchi, T ; Adachi, A ; Nomiyama, H ; Ishizaki, A ; Nakayama, J ; Sonomoto, K</creator><creatorcontrib>Aso, Y. (Kyushu Univ., Fukuoka (Japan)) ; Sashihara, T ; Nagao, J ; Kanemasa, Y ; Koga, H ; Hashimoto, T ; Higuchi, T ; Adachi, A ; Nomiyama, H ; Ishizaki, A ; Nakayama, J ; Sonomoto, K</creatorcontrib><description>We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA (a structural gene), -M, -T, -F, -E, -G, and two open reading frames, ORF1 and ORF7. NukM and NukT were predicted to be involved in post-translational modification and secretion of nukacin ISK-1 respectively. NukF, -E, and -G were predicted to form a membrane complex which contributes to self-protection from nukacin ISK-1. Transcriptional analyses revealed that nukM through ORF7 comprises an operon, and that ORF1 is transcribed independently from downstream of nukA. The transcriptional levels of the nukA and nukM genes were enhanced by osmotic stress. The expression level of the nukA transcript was scarcely enhanced by nukacin ISK-1, suggesting that expression is not under the control of the autoregulatory circuit.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.68.1663</identifier><identifier>PMID: 15322349</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Amino Acid Sequence ; ANTIBIOTICS ; bacteriocin ; Bacteriocins - genetics ; Bacteriocins - metabolism ; Base Sequence ; Biological and medical sciences ; BIOSYNTHESIS ; CLASSIFICATION ; Fundamental and applied biological sciences. Psychology ; gene cluster ; GENES ; Genes, Bacterial - genetics ; IMMUNITY ; lantibiotic ; MOLECULAR CLONING ; Molecular Sequence Data ; nukacin ISK-1 ; Open Reading Frames - genetics ; PCR ; PLASMIDS ; Plasmids - genetics ; STAPHYLOCOCCUS ; Staphylococcus - genetics ; Staphylococcus - metabolism ; TRANSCRIPTION</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2004-08, Vol.68 (8), p.1663-1671</ispartof><rights>2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2004</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-18270d8c00bd88492f84c2ee27b88f8aa18b6d16f368f9b089f20a7908a252553</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16238425$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15322349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aso, Y. (Kyushu Univ., Fukuoka (Japan))</creatorcontrib><creatorcontrib>Sashihara, T</creatorcontrib><creatorcontrib>Nagao, J</creatorcontrib><creatorcontrib>Kanemasa, Y</creatorcontrib><creatorcontrib>Koga, H</creatorcontrib><creatorcontrib>Hashimoto, T</creatorcontrib><creatorcontrib>Higuchi, T</creatorcontrib><creatorcontrib>Adachi, A</creatorcontrib><creatorcontrib>Nomiyama, H</creatorcontrib><creatorcontrib>Ishizaki, A</creatorcontrib><creatorcontrib>Nakayama, J</creatorcontrib><creatorcontrib>Sonomoto, K</creatorcontrib><title>Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA (a structural gene), -M, -T, -F, -E, -G, and two open reading frames, ORF1 and ORF7. NukM and NukT were predicted to be involved in post-translational modification and secretion of nukacin ISK-1 respectively. NukF, -E, and -G were predicted to form a membrane complex which contributes to self-protection from nukacin ISK-1. Transcriptional analyses revealed that nukM through ORF7 comprises an operon, and that ORF1 is transcribed independently from downstream of nukA. The transcriptional levels of the nukA and nukM genes were enhanced by osmotic stress. The expression level of the nukA transcript was scarcely enhanced by nukacin ISK-1, suggesting that expression is not under the control of the autoregulatory circuit.</description><subject>Amino Acid Sequence</subject><subject>ANTIBIOTICS</subject><subject>bacteriocin</subject><subject>Bacteriocins - genetics</subject><subject>Bacteriocins - metabolism</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>BIOSYNTHESIS</subject><subject>CLASSIFICATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene cluster</subject><subject>GENES</subject><subject>Genes, Bacterial - genetics</subject><subject>IMMUNITY</subject><subject>lantibiotic</subject><subject>MOLECULAR CLONING</subject><subject>Molecular Sequence Data</subject><subject>nukacin ISK-1</subject><subject>Open Reading Frames - genetics</subject><subject>PCR</subject><subject>PLASMIDS</subject><subject>Plasmids - genetics</subject><subject>STAPHYLOCOCCUS</subject><subject>Staphylococcus - genetics</subject><subject>Staphylococcus - metabolism</subject><subject>TRANSCRIPTION</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9rFDEUB_BBFLutnjwrAdGLzppfk8kcZalaLShUz-FNJtlNnUnWJENZ_wj_ZrPdlYIInhKSz3u8x7eqnhC8JLQlb_q-Xwq5JEKwe9WCMN7WouPt_WqBOyJqyRtyUp2mdI1xeWjIw-qENIxSxrtF9Wu1gQg6m-h-QnbBo2ARoLXxBulxTuVj_3KVYbvZjUEHreeEbiD6UoEurj7VBBmvw-D8GuWNQb0LaefLLbl028sPyE3T7F3eoRxuzQg-uwKz06-Rn7-Ddv7Q61H1wMKYzOPjeVZ9e3f-dfWhvvz8_mL19rLWjehyTSRt8SA1xv0gJe-olVxTY2jbS2klAJG9GIiwTEjb9Vh2lmJoOyyBNrRp2Fn18tB3G8OP2aSsJpe0GctkJsxJCSExFVj8F5JWcMIELvD5X_A6zNGXJRThvJMEM0KLenVQOoaUorFqG90EcacIVvs0VUlTCan2aRb97Nhz7icz3NljfAW8OAJIGkYbwWuX7pygTHK6X1ccnPM2xAluQhwHlaEkGv8UsX9P8PRQaCEoWMfiPn6hGDcYM8pa9ht1OsI-</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Aso, Y. (Kyushu Univ., Fukuoka (Japan))</creator><creator>Sashihara, T</creator><creator>Nagao, J</creator><creator>Kanemasa, Y</creator><creator>Koga, H</creator><creator>Hashimoto, T</creator><creator>Higuchi, T</creator><creator>Adachi, A</creator><creator>Nomiyama, H</creator><creator>Ishizaki, A</creator><creator>Nakayama, J</creator><creator>Sonomoto, K</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1</title><author>Aso, Y. (Kyushu Univ., Fukuoka (Japan)) ; Sashihara, T ; Nagao, J ; Kanemasa, Y ; Koga, H ; Hashimoto, T ; Higuchi, T ; Adachi, A ; Nomiyama, H ; Ishizaki, A ; Nakayama, J ; Sonomoto, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-18270d8c00bd88492f84c2ee27b88f8aa18b6d16f368f9b089f20a7908a252553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>ANTIBIOTICS</topic><topic>bacteriocin</topic><topic>Bacteriocins - genetics</topic><topic>Bacteriocins - metabolism</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>BIOSYNTHESIS</topic><topic>CLASSIFICATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene cluster</topic><topic>GENES</topic><topic>Genes, Bacterial - genetics</topic><topic>IMMUNITY</topic><topic>lantibiotic</topic><topic>MOLECULAR CLONING</topic><topic>Molecular Sequence Data</topic><topic>nukacin ISK-1</topic><topic>Open Reading Frames - genetics</topic><topic>PCR</topic><topic>PLASMIDS</topic><topic>Plasmids - genetics</topic><topic>STAPHYLOCOCCUS</topic><topic>Staphylococcus - genetics</topic><topic>Staphylococcus - metabolism</topic><topic>TRANSCRIPTION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aso, Y. 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(Kyushu Univ., Fukuoka (Japan))</au><au>Sashihara, T</au><au>Nagao, J</au><au>Kanemasa, Y</au><au>Koga, H</au><au>Hashimoto, T</au><au>Higuchi, T</au><au>Adachi, A</au><au>Nomiyama, H</au><au>Ishizaki, A</au><au>Nakayama, J</au><au>Sonomoto, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>68</volume><issue>8</issue><spage>1663</spage><epage>1671</epage><pages>1663-1671</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA (a structural gene), -M, -T, -F, -E, -G, and two open reading frames, ORF1 and ORF7. NukM and NukT were predicted to be involved in post-translational modification and secretion of nukacin ISK-1 respectively. NukF, -E, and -G were predicted to form a membrane complex which contributes to self-protection from nukacin ISK-1. Transcriptional analyses revealed that nukM through ORF7 comprises an operon, and that ORF1 is transcribed independently from downstream of nukA. The transcriptional levels of the nukA and nukM genes were enhanced by osmotic stress. The expression level of the nukA transcript was scarcely enhanced by nukacin ISK-1, suggesting that expression is not under the control of the autoregulatory circuit.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>15322349</pmid><doi>10.1271/bbb.68.1663</doi><tpages>9</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2004-08, Vol.68 (8), p.1663-1671 |
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| source | MEDLINE; Full-Text Journals in Chemistry (Open access); Freely Accessible Japanese Titles; Oxford University Press; EZB Electronic Journals Library; J-STAGE |
| subjects | Amino Acid Sequence ANTIBIOTICS bacteriocin Bacteriocins - genetics Bacteriocins - metabolism Base Sequence Biological and medical sciences BIOSYNTHESIS CLASSIFICATION Fundamental and applied biological sciences. Psychology gene cluster GENES Genes, Bacterial - genetics IMMUNITY lantibiotic MOLECULAR CLONING Molecular Sequence Data nukacin ISK-1 Open Reading Frames - genetics PCR PLASMIDS Plasmids - genetics STAPHYLOCOCCUS Staphylococcus - genetics Staphylococcus - metabolism TRANSCRIPTION |
| title | Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1 |
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