Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1
Culture conditions for pullulan production by Aureobasidium pullulans were optimized using response surface methodology at shake flask level without pH control. In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2009, Vol.152 (1), p.42-53 |
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| creator | Singh, Ram Sarup Singh, Harpreet Saini, Gaganpreet Kaur |
| description | Culture conditions for pullulan production by Aureobasidium pullulans were optimized using response surface methodology at shake flask level without pH control. In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the levels of five factors significantly affecting the pullulan production, biomass production, and sugar utilization in submerged cultivation. The selected factors included concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride. Using this methodology, the optimal values for concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride were 5.31%, 0.11%, 0.07%, 0.05%, and 0.15% (w/v), respectively. This optimized medium has projected a theoretically production of pullulan of 4.44%, biomass yield of 1.03%, and sugar utilization of 97.12%. The multiple correlation coefficient 'R' was 0.9976, 0.9761 and 0.9919 for pullulan production, biomass production, and sugar utilization, respectively. The value of R being very close to one justifies an excellent correlation between the predicted and the experimental data. |
| doi_str_mv | 10.1007/s12010-008-8180-9 |
| format | Article |
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In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the levels of five factors significantly affecting the pullulan production, biomass production, and sugar utilization in submerged cultivation. The selected factors included concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride. Using this methodology, the optimal values for concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride were 5.31%, 0.11%, 0.07%, 0.05%, and 0.15% (w/v), respectively. This optimized medium has projected a theoretically production of pullulan of 4.44%, biomass yield of 1.03%, and sugar utilization of 97.12%. The multiple correlation coefficient 'R' was 0.9976, 0.9761 and 0.9919 for pullulan production, biomass production, and sugar utilization, respectively. The value of R being very close to one justifies an excellent correlation between the predicted and the experimental data.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-008-8180-9</identifier><identifier>PMID: 18427743</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>New York: New York : Humana Press Inc</publisher><subject>Ammonium ; ammonium sulfate ; Ammonium Sulfate - pharmacology ; Ascomycota - drug effects ; Ascomycota - metabolism ; Aureobasidium pullulans ; Biochemistry ; Biological and medical sciences ; Biomass ; Biotechnology ; Chemical compounds ; Chemistry ; Chemistry and Materials Science ; Correlation coefficient ; culture media ; Culture Media - chemistry ; Culture Media - pharmacology ; dipotassium hydrogen phosphate ; Fundamental and applied biological sciences. Psychology ; Glucans - biosynthesis ; Models, Biological ; optimization ; Phosphates - pharmacology ; Polymers ; Potassium Compounds - pharmacology ; pullulan ; response surface methodology ; Sodium chloride ; Sodium Chloride - pharmacology ; Studies ; submerged fermentation ; sucrose ; Sucrose - pharmacology ; Sugar ; Sulfates ; Yeasts ; Yeasts - chemistry ; yields</subject><ispartof>Applied biochemistry and biotechnology, 2009, Vol.152 (1), p.42-53</ispartof><rights>Humana Press 2008</rights><rights>2009 INIST-CNRS</rights><rights>Humana Press 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-2eba6f0934db90dc6747375b7638968dd55ff84c46fd206e0961cdd0d32137b53</citedby><cites>FETCH-LOGICAL-c453t-2eba6f0934db90dc6747375b7638968dd55ff84c46fd206e0961cdd0d32137b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-008-8180-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-008-8180-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21039733$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18427743$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, Ram Sarup</creatorcontrib><creatorcontrib>Singh, Harpreet</creatorcontrib><creatorcontrib>Saini, Gaganpreet Kaur</creatorcontrib><title>Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Culture conditions for pullulan production by Aureobasidium pullulans were optimized using response surface methodology at shake flask level without pH control. In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the levels of five factors significantly affecting the pullulan production, biomass production, and sugar utilization in submerged cultivation. The selected factors included concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride. Using this methodology, the optimal values for concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride were 5.31%, 0.11%, 0.07%, 0.05%, and 0.15% (w/v), respectively. This optimized medium has projected a theoretically production of pullulan of 4.44%, biomass yield of 1.03%, and sugar utilization of 97.12%. The multiple correlation coefficient 'R' was 0.9976, 0.9761 and 0.9919 for pullulan production, biomass production, and sugar utilization, respectively. The value of R being very close to one justifies an excellent correlation between the predicted and the experimental data.</description><subject>Ammonium</subject><subject>ammonium sulfate</subject><subject>Ammonium Sulfate - pharmacology</subject><subject>Ascomycota - drug effects</subject><subject>Ascomycota - metabolism</subject><subject>Aureobasidium pullulans</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Biotechnology</subject><subject>Chemical compounds</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Correlation coefficient</subject><subject>culture media</subject><subject>Culture Media - chemistry</subject><subject>Culture Media - pharmacology</subject><subject>dipotassium hydrogen phosphate</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucans - biosynthesis</subject><subject>Models, Biological</subject><subject>optimization</subject><subject>Phosphates - pharmacology</subject><subject>Polymers</subject><subject>Potassium Compounds - pharmacology</subject><subject>pullulan</subject><subject>response surface methodology</subject><subject>Sodium chloride</subject><subject>Sodium Chloride - pharmacology</subject><subject>Studies</subject><subject>submerged fermentation</subject><subject>sucrose</subject><subject>Sucrose - pharmacology</subject><subject>Sugar</subject><subject>Sulfates</subject><subject>Yeasts</subject><subject>Yeasts - chemistry</subject><subject>yields</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1u1TAQRiMEopfCA7ABs4BdYGzHf8tyRQGpqBWla8uJ7ZIqiYMdC5Wnx7eJqMQCVrPwOd-M9VXVcwxvMYB4lzABDDWArCWWUKsH1Q4zpmogCj-sdkAErQmR6qh6ktINACaSicfVEZYNEaKhu-rnV5fmMCWHLnP0pnPofF76sf9llj5MKHi0fHdoH_ul78yAvjjb5xHtw1gkNy0J-RDRRR6GPJgJXcRgc3dntrfoJEcXWpP6O2feoIRO39f4afXImyG5Z9s8rq5OP3zbf6rPzj9-3p-c1V3D6FIT1xruQdHGtgpsx0UjqGCt4FQqLq1lzHvZdA33lgB3oDjurAVLCaaiZfS4erPmzjH8yC4teuxT54ZyiAs5ac6FUiDhv2BJx4wDL-Crv8CbkONUPqGlwpIQjguDV6aLIaXovJ5jP5p4qzHoQ3V6rU6X6vShOq2K82LLze3o7L2xdVWA1xtgUunCRzN1ffrDEQxUCXrgyMql8jRdu3h_4L-2v1wlb4I217EEX10WiAJmQkki6W9WELoC</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Singh, Ram Sarup</creator><creator>Singh, Harpreet</creator><creator>Saini, Gaganpreet Kaur</creator><general>New York : Humana Press Inc</general><general>Humana Press Inc</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7QO</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>2009</creationdate><title>Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1</title><author>Singh, Ram Sarup ; Singh, Harpreet ; Saini, Gaganpreet Kaur</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-2eba6f0934db90dc6747375b7638968dd55ff84c46fd206e0961cdd0d32137b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Ammonium</topic><topic>ammonium sulfate</topic><topic>Ammonium Sulfate - pharmacology</topic><topic>Ascomycota - drug effects</topic><topic>Ascomycota - metabolism</topic><topic>Aureobasidium pullulans</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>Biotechnology</topic><topic>Chemical compounds</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Correlation coefficient</topic><topic>culture media</topic><topic>Culture Media - chemistry</topic><topic>Culture Media - pharmacology</topic><topic>dipotassium hydrogen phosphate</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucans - biosynthesis</topic><topic>Models, Biological</topic><topic>optimization</topic><topic>Phosphates - pharmacology</topic><topic>Polymers</topic><topic>Potassium Compounds - pharmacology</topic><topic>pullulan</topic><topic>response surface methodology</topic><topic>Sodium chloride</topic><topic>Sodium Chloride - pharmacology</topic><topic>Studies</topic><topic>submerged fermentation</topic><topic>sucrose</topic><topic>Sucrose - pharmacology</topic><topic>Sugar</topic><topic>Sulfates</topic><topic>Yeasts</topic><topic>Yeasts - chemistry</topic><topic>yields</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Ram Sarup</creatorcontrib><creatorcontrib>Singh, Harpreet</creatorcontrib><creatorcontrib>Saini, Gaganpreet Kaur</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Ram Sarup</au><au>Singh, Harpreet</au><au>Saini, Gaganpreet Kaur</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2009</date><risdate>2009</risdate><volume>152</volume><issue>1</issue><spage>42</spage><epage>53</epage><pages>42-53</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><coden>ABIBDL</coden><abstract>Culture conditions for pullulan production by Aureobasidium pullulans were optimized using response surface methodology at shake flask level without pH control. In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the levels of five factors significantly affecting the pullulan production, biomass production, and sugar utilization in submerged cultivation. The selected factors included concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride. Using this methodology, the optimal values for concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride were 5.31%, 0.11%, 0.07%, 0.05%, and 0.15% (w/v), respectively. This optimized medium has projected a theoretically production of pullulan of 4.44%, biomass yield of 1.03%, and sugar utilization of 97.12%. The multiple correlation coefficient 'R' was 0.9976, 0.9761 and 0.9919 for pullulan production, biomass production, and sugar utilization, respectively. The value of R being very close to one justifies an excellent correlation between the predicted and the experimental data.</abstract><cop>New York</cop><pub>New York : Humana Press Inc</pub><pmid>18427743</pmid><doi>10.1007/s12010-008-8180-9</doi><tpages>12</tpages></addata></record> |
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| ispartof | Applied biochemistry and biotechnology, 2009, Vol.152 (1), p.42-53 |
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| subjects | Ammonium ammonium sulfate Ammonium Sulfate - pharmacology Ascomycota - drug effects Ascomycota - metabolism Aureobasidium pullulans Biochemistry Biological and medical sciences Biomass Biotechnology Chemical compounds Chemistry Chemistry and Materials Science Correlation coefficient culture media Culture Media - chemistry Culture Media - pharmacology dipotassium hydrogen phosphate Fundamental and applied biological sciences. Psychology Glucans - biosynthesis Models, Biological optimization Phosphates - pharmacology Polymers Potassium Compounds - pharmacology pullulan response surface methodology Sodium chloride Sodium Chloride - pharmacology Studies submerged fermentation sucrose Sucrose - pharmacology Sugar Sulfates Yeasts Yeasts - chemistry yields |
| title | Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1 |
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