Expression, Purification, and Characterization of Phosphatidylserine Synthase from Escherichia coli K12 in Bacillus subtilis

Although phosphatidylserine synthase (PSS) from Escherichia coli is an ideal enzyme for phospholipid production, its application in the food industry has been limited because of the low PSS yield. In this study, the pss gene was cloned from E. coli K12 and expressed in Bacillus subtilis DB104, and t...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2009-01, Vol.57 (1), p.122-126
Hauptverfasser:	Zhang, Ye-Ni, Lu, Fu-Ping, Chen, Guan-Qun, Li, Yu, Wang, Jian-Ling
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacillus subtilis Bacillus subtilis - genetics Biological and medical sciences CDPdiacylglycerol-Serine O-Phosphatidyltransferase - genetics CDPdiacylglycerol-Serine O-Phosphatidyltransferase - isolation & purification Chemical Aspects of Biotechnology/Molecular Biology Cloning, Molecular enzyme activity Enzyme Stability Escherichia coli K12 Escherichia coli K12 - enzymology food biotechnology Food industries Food microbiology Fundamental and applied biological sciences. Psychology Gene Expression gene transfer genes Hot Temperature Hydrogen-Ion Concentration molecular cloning Molecular Sequence Data phosphatidylcholines phosphatidylserine synthase phosphatidylserines pss gene recombinant proteins Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism transferases Transformation, Bacterial
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