Principle and applications of the protein-purification-parameter screening system
For the purification of a target protein, liquid chromatography is the method of choice, if its activity has to be maintained. The selection of optimum parameters will improve in proportion to the number of individual parameters varied in initial experiments. Here a fast screening method is describe...
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| Veröffentlicht in: | Journal of Chromatography A 2004-07, Vol.1043 (1), p.73-80 |
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| container_end_page | 80 |
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| container_issue | 1 |
| container_start_page | 73 |
| container_title | Journal of Chromatography A |
| container_volume | 1043 |
| creator | Thiemann, Joachim Jankowski, Joachim Rykl, Jana Kurzawski, Sandra Pohl, Thomas Wittmann-Liebold, Brigitte Schlüter, Hartmut |
| description | For the purification of a target protein, liquid chromatography is the method of choice, if its activity has to be maintained. The selection of optimum parameters will improve in proportion to the number of individual parameters varied in initial experiments. Here a fast screening method is described, which utilizes automated parallel chromatographic experiments in the batch mode in 96-well plates. The principle of this protein-purification-parameter screening (PPS) system is demonstrated with a mixture of four proteins. An application of PPS for the determination of a purification step of an angiotensin-II-generating enzyme from a crude tissue extract is shown. |
| doi_str_mv | 10.1016/j.chroma.2004.05.074 |
| format | Article |
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The selection of optimum parameters will improve in proportion to the number of individual parameters varied in initial experiments. Here a fast screening method is described, which utilizes automated parallel chromatographic experiments in the batch mode in 96-well plates. The principle of this protein-purification-parameter screening (PPS) system is demonstrated with a mixture of four proteins. An application of PPS for the determination of a purification step of an angiotensin-II-generating enzyme from a crude tissue extract is shown.</description><subject>Analysis</subject><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical chemistry</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Kidney - chemistry</subject><subject>Medical sciences</subject><subject>Pharmacology. 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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis Analytical biochemistry: general aspects, technics, instrumentation Analytical chemistry Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Ion Exchange - methods Electrophoresis, Gel, Two-Dimensional Exact sciences and technology Fundamental and applied biological sciences. Psychology General pharmacology Kidney - chemistry Medical sciences Pharmacology. Drug treatments Protein-purification-parameter screening Proteins Proteins - isolation & purification Swine |
| title | Principle and applications of the protein-purification-parameter screening system |
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