Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice
1 Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea 2 Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republi...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2009-01, Vol.59 (1), p.22-27 |
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| creator | Madhaiyan, Munusamy Poonguzhali, Selvaraj Kwon, Soon-Wo Sa, Tong-Min |
| description | 1 Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea
2 Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea
Correspondence Tong-Min Sa tomsa{at}chungbuk.ac.kr
A pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, CBMB27 T , isolated from leaf tissues of rice ( Oryza sativa L. Dong-Jin), was analysed using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium oryzae , Methylobacterium fujisawaense and Methylobacterium mesophilicum ; strain CBMB27 T showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strains of these three species. DNA–DNA hybridization experiments revealed low levels ( |
| doi_str_mv | 10.1099/ijs.0.001693-0 |
| format | Article |
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2 Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea
Correspondence Tong-Min Sa tomsa{at}chungbuk.ac.kr
A pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, CBMB27 T , isolated from leaf tissues of rice ( Oryza sativa L. Dong-Jin), was analysed using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium oryzae , Methylobacterium fujisawaense and Methylobacterium mesophilicum ; strain CBMB27 T showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strains of these three species. DNA–DNA hybridization experiments revealed low levels (<38 %) of DNA–DNA relatedness between strain CBMB27 T and its closest relatives. The sequence of the 1-aminocyclopropane-1-carboxylate deaminase gene ( acdS ) in strain CBMB27 T differed from those of close relatives. The major fatty acid of the isolate was C 18 : 1 7 c and the G+C content of the genomic DNA was 66.8 mol%. Based on the results of 16S rRNA gene sequence analysis, DNA–DNA hybridization, and physiological and biochemical characterization, which enabled the isolate to be differentiated from all recognized species of the genus Methylobacterium , it was concluded that strain CBMB27 T represents a novel species in the genus Methylobacterium for which the name Methylobacterium phyllosphaerae sp. nov. is proposed (type strain CBMB27 T =LMG 24361 T =KACC 11716 T =DSM 19779 T ).
Abbreviations: ACCD, 1-aminocyclopropane-1-carboxylate deaminase
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, mxaF and acdS gene sequences of strain CBMB27 T are EF126746 , EF562496 and EU430438 , respectively.
API ZYM reactions of strain CBMB27 T and related strains are available as supplementary material with the online version of this paper.</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.001693-0</identifier><identifier>PMID: 19126717</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Bacterial Typing Techniques ; Bacteriology ; Biological and medical sciences ; Carbon-Carbon Lyases - genetics ; DNA, Bacterial - analysis ; DNA, Ribosomal - analysis ; Fundamental and applied biological sciences. Psychology ; Genes, rRNA ; Korea ; Methylobacterium - classification ; Methylobacterium - genetics ; Methylobacterium - isolation & purification ; Methylobacterium - physiology ; Microbiology ; Miscellaneous ; Nucleic Acid Hybridization ; Oryza - microbiology ; Phenotype ; Phylogeny ; Pigmentation ; Plant Leaves - microbiology ; RNA, Ribosomal, 16S ; Sequence Analysis, DNA ; Species Specificity ; Systematics</subject><ispartof>International journal of systematic and evolutionary microbiology, 2009-01, Vol.59 (1), p.22-27</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-46209f38c45e6139f2ebe128a18727a960a42f9e21315dcf25171efa84e4bf403</citedby><cites>FETCH-LOGICAL-c460t-46209f38c45e6139f2ebe128a18727a960a42f9e21315dcf25171efa84e4bf403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21018167$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19126717$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Madhaiyan, Munusamy</creatorcontrib><creatorcontrib>Poonguzhali, Selvaraj</creatorcontrib><creatorcontrib>Kwon, Soon-Wo</creatorcontrib><creatorcontrib>Sa, Tong-Min</creatorcontrib><title>Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>1 Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea
2 Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea
Correspondence Tong-Min Sa tomsa{at}chungbuk.ac.kr
A pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, CBMB27 T , isolated from leaf tissues of rice ( Oryza sativa L. Dong-Jin), was analysed using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium oryzae , Methylobacterium fujisawaense and Methylobacterium mesophilicum ; strain CBMB27 T showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strains of these three species. DNA–DNA hybridization experiments revealed low levels (<38 %) of DNA–DNA relatedness between strain CBMB27 T and its closest relatives. The sequence of the 1-aminocyclopropane-1-carboxylate deaminase gene ( acdS ) in strain CBMB27 T differed from those of close relatives. The major fatty acid of the isolate was C 18 : 1 7 c and the G+C content of the genomic DNA was 66.8 mol%. Based on the results of 16S rRNA gene sequence analysis, DNA–DNA hybridization, and physiological and biochemical characterization, which enabled the isolate to be differentiated from all recognized species of the genus Methylobacterium , it was concluded that strain CBMB27 T represents a novel species in the genus Methylobacterium for which the name Methylobacterium phyllosphaerae sp. nov. is proposed (type strain CBMB27 T =LMG 24361 T =KACC 11716 T =DSM 19779 T ).
Abbreviations: ACCD, 1-aminocyclopropane-1-carboxylate deaminase
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, mxaF and acdS gene sequences of strain CBMB27 T are EF126746 , EF562496 and EU430438 , respectively.
API ZYM reactions of strain CBMB27 T and related strains are available as supplementary material with the online version of this paper.</description><subject>Bacterial Typing Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carbon-Carbon Lyases - genetics</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Ribosomal - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, rRNA</subject><subject>Korea</subject><subject>Methylobacterium - classification</subject><subject>Methylobacterium - genetics</subject><subject>Methylobacterium - isolation & purification</subject><subject>Methylobacterium - physiology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Nucleic Acid Hybridization</subject><subject>Oryza - microbiology</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Pigmentation</subject><subject>Plant Leaves - microbiology</subject><subject>RNA, Ribosomal, 16S</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><subject>Systematics</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEFv1DAQRi0EomXhyhH5Aqcm9TiOEx-rihakol7K2XK8441LEqe2U9R_36x21T3NjPT05tNHyFdgJTClLv1jKlnJGEhVFewdOQchZVGzSrx_27k8I59SelwpIRn7SM5AAZcNNOck_sHcvwyhMzZj9MtI5_UcQpp7g9EgTXNJp_BcXlBDZz_9K2a_G3HKuL2gzthlyCb7Z6TjwZNjmHvqYhhp7vEkw4g0OBq9xc_kgzNDwi_HuSF_b34-XP8q7u5vf19f3RV2TZkLITlTrmqtqFFCpRzHDoG3BtqGN0ZJZgR3CjlUUG-t4zU0gM60AkXnBKs25MfBO8fwtGDKevTJ4jCYCcOStJSNkmJVb0h5AG0MKUV0eo5-NPFFA9P7lvXasmb60LLem78dzUs34vaEH2tdge9HwCRrBhfNZH164zgwaEE2p4i93_X_fUS9w2n0a47Oh_3XWmnQnFevBRmUkA</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Madhaiyan, Munusamy</creator><creator>Poonguzhali, Selvaraj</creator><creator>Kwon, Soon-Wo</creator><creator>Sa, Tong-Min</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090101</creationdate><title>Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice</title><author>Madhaiyan, Munusamy ; Poonguzhali, Selvaraj ; Kwon, Soon-Wo ; Sa, Tong-Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-46209f38c45e6139f2ebe128a18727a960a42f9e21315dcf25171efa84e4bf403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Bacterial Typing Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Carbon-Carbon Lyases - genetics</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Ribosomal - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, rRNA</topic><topic>Korea</topic><topic>Methylobacterium - classification</topic><topic>Methylobacterium - genetics</topic><topic>Methylobacterium - isolation & purification</topic><topic>Methylobacterium - physiology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Nucleic Acid Hybridization</topic><topic>Oryza - microbiology</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>Pigmentation</topic><topic>Plant Leaves - microbiology</topic><topic>RNA, Ribosomal, 16S</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><topic>Systematics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Madhaiyan, Munusamy</creatorcontrib><creatorcontrib>Poonguzhali, Selvaraj</creatorcontrib><creatorcontrib>Kwon, Soon-Wo</creatorcontrib><creatorcontrib>Sa, Tong-Min</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Madhaiyan, Munusamy</au><au>Poonguzhali, Selvaraj</au><au>Kwon, Soon-Wo</au><au>Sa, Tong-Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2009-01-01</date><risdate>2009</risdate><volume>59</volume><issue>1</issue><spage>22</spage><epage>27</epage><pages>22-27</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>1 Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea
2 Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea
Correspondence Tong-Min Sa tomsa{at}chungbuk.ac.kr
A pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, CBMB27 T , isolated from leaf tissues of rice ( Oryza sativa L. Dong-Jin), was analysed using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium oryzae , Methylobacterium fujisawaense and Methylobacterium mesophilicum ; strain CBMB27 T showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strains of these three species. DNA–DNA hybridization experiments revealed low levels (<38 %) of DNA–DNA relatedness between strain CBMB27 T and its closest relatives. The sequence of the 1-aminocyclopropane-1-carboxylate deaminase gene ( acdS ) in strain CBMB27 T differed from those of close relatives. The major fatty acid of the isolate was C 18 : 1 7 c and the G+C content of the genomic DNA was 66.8 mol%. Based on the results of 16S rRNA gene sequence analysis, DNA–DNA hybridization, and physiological and biochemical characterization, which enabled the isolate to be differentiated from all recognized species of the genus Methylobacterium , it was concluded that strain CBMB27 T represents a novel species in the genus Methylobacterium for which the name Methylobacterium phyllosphaerae sp. nov. is proposed (type strain CBMB27 T =LMG 24361 T =KACC 11716 T =DSM 19779 T ).
Abbreviations: ACCD, 1-aminocyclopropane-1-carboxylate deaminase
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, mxaF and acdS gene sequences of strain CBMB27 T are EF126746 , EF562496 and EU430438 , respectively.
API ZYM reactions of strain CBMB27 T and related strains are available as supplementary material with the online version of this paper.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>19126717</pmid><doi>10.1099/ijs.0.001693-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Bacterial Typing Techniques Bacteriology Biological and medical sciences Carbon-Carbon Lyases - genetics DNA, Bacterial - analysis DNA, Ribosomal - analysis Fundamental and applied biological sciences. Psychology Genes, rRNA Korea Methylobacterium - classification Methylobacterium - genetics Methylobacterium - isolation & purification Methylobacterium - physiology Microbiology Miscellaneous Nucleic Acid Hybridization Oryza - microbiology Phenotype Phylogeny Pigmentation Plant Leaves - microbiology RNA, Ribosomal, 16S Sequence Analysis, DNA Species Specificity Systematics |
| title | Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice |
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