Synovial expression of osteopontin correlates with angiogenesis in juvenile idiopathic arthritis
Objective. To evaluate the synovial expression of osteopontin (OPN) and its possible correlation with the degree of synovial angiogenesis in human chronic idiopathic arthritis. Methods. Forty-five patients with active juvenile idiopathic arthritis (JIA) were studied. All patients underwent SF aspira...
Gespeichert in:
| Veröffentlicht in: | British journal of rheumatology 2004-09, Vol.43 (9), p.1091-1096 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1096 |
|---|---|
| container_issue | 9 |
| container_start_page | 1091 |
| container_title | British journal of rheumatology |
| container_volume | 43 |
| creator | Gattorno, M. Gregorio, A. Ferlito, F. Gerloni, V. Parafioriti, A. Felici, E. Sala, E. Gambini, C. Picco, P. Martini, A. |
| description | Objective. To evaluate the synovial expression of osteopontin (OPN) and its possible correlation with the degree of synovial angiogenesis in human chronic idiopathic arthritis. Methods. Forty-five patients with active juvenile idiopathic arthritis (JIA) were studied. All patients underwent SF aspiration before steroid injection. A paired plasma sample was collected from 22 JIA patients. Plasma from 15 age-matched healthy subjects was used as control. Plasma and SF were tested by ELISA for OPN and vascular endothelial growth factor (VEGF). Synovial tissue was obtained at synovectomy from 10 JIA patients. Immunohistochemistry was performed according to a standard technique with anti-OPN, anti-CD68, anti-CD31 anti-VEGF and anti-αvβ3 antibodies. Results. OPN levels were significantly higher in SF than in paired plasma samples (P |
| doi_str_mv | 10.1093/rheumatology/keh250 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66795920</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17882190</sourcerecordid><originalsourceid>FETCH-LOGICAL-c498t-6cf80cf1628ed58402c9321f403d3ed814840e8be3439b4e7030cdb5b1db4b833</originalsourceid><addsrcrecordid>eNqF0d9rFDEQB_BFLLZW_wJBFsG-rc3vTR6laM-jULAWii8xm529zXUvWZNs2_vv3XJHK774NGHmMwPhWxTvMPqEkaKnsYdpY3IYwmp7egs94ehFcYSZIBWilLx8ehN2WLxOaY0Q4pjKV8Uh5lgpguVR8etq68OdM0MJD2OElFzwZejKkDKEMfjsfGlDjDCYDKm8d7kvjV-5sAIPyaVynq-nO_BugNK1Lowm986WJuY-uuzSm-KgM0OCt_t6XFx__fLjbFFdXJ5_O_t8UVmmZK6E7SSyHRZEQsslQ8QqSnDHEG0ptBKzuQeyAcqoahjUiCLbNrzBbcMaSelxcbK7O8bwe4KU9cYlC8NgPIQpaSFqxRVB_4W4lpJg9Qg__APXYYp-_oTGiotaCi5nRHfIxpBShE6P0W1M3GqM9GNM-u-Y9C6meev9_vTUbKB93tnnMoOPe2CSNUMXjbcuPTuBVC1YPbtq59yc18PT3MRbLWpac724-akXy-UVxsvv-pz-AbAYsLg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>195678658</pqid></control><display><type>article</type><title>Synovial expression of osteopontin correlates with angiogenesis in juvenile idiopathic arthritis</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Gattorno, M. ; Gregorio, A. ; Ferlito, F. ; Gerloni, V. ; Parafioriti, A. ; Felici, E. ; Sala, E. ; Gambini, C. ; Picco, P. ; Martini, A.</creator><creatorcontrib>Gattorno, M. ; Gregorio, A. ; Ferlito, F. ; Gerloni, V. ; Parafioriti, A. ; Felici, E. ; Sala, E. ; Gambini, C. ; Picco, P. ; Martini, A.</creatorcontrib><description>Objective. To evaluate the synovial expression of osteopontin (OPN) and its possible correlation with the degree of synovial angiogenesis in human chronic idiopathic arthritis. Methods. Forty-five patients with active juvenile idiopathic arthritis (JIA) were studied. All patients underwent SF aspiration before steroid injection. A paired plasma sample was collected from 22 JIA patients. Plasma from 15 age-matched healthy subjects was used as control. Plasma and SF were tested by ELISA for OPN and vascular endothelial growth factor (VEGF). Synovial tissue was obtained at synovectomy from 10 JIA patients. Immunohistochemistry was performed according to a standard technique with anti-OPN, anti-CD68, anti-CD31 anti-VEGF and anti-αvβ3 antibodies. Results. OPN levels were significantly higher in SF than in paired plasma samples (P<0.001). The same pattern was observed for VEGF (P<0.001). A positive correlation between OPN and VEGF concentrations was found in SF (r = 0.6, P = 0.001). In synovial tissue, OPN was expressed at the level of the lining and sublining layers with a distribution similar to that observed for VEGF. OPN expression in the lining layer correlated with the number of vessels present in the areas underlying the sublining layer. Conclusions. Synovial expression of OPN correlates with parameters of angiogenesis in JIA. These data support, in human disease, the possible role of OPN in the vascularization of inflamed synovial tissue, as previously shown in OPN-deficient animal models of arthritis.</description><identifier>ISSN: 1462-0324</identifier><identifier>ISSN: 1460-2172</identifier><identifier>EISSN: 1462-0332</identifier><identifier>EISSN: 1460-2172</identifier><identifier>DOI: 10.1093/rheumatology/keh250</identifier><identifier>PMID: 15199218</identifier><identifier>CODEN: BJRHDF</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adolescent ; Adult ; Angiogenesis ; Antigens, CD - analysis ; Arthritis, Juvenile - metabolism ; Arthritis, Juvenile - physiopathology ; Biological and medical sciences ; Child ; Diseases of the osteoarticular system ; Female ; Humans ; Immunohistochemistry - methods ; Inflammatory joint diseases ; Integrins ; Integrins - analysis ; Joints - blood supply ; Juvenile idiopathic arthritis ; Medical sciences ; Neovascularization, Pathologic - physiopathology ; Osteopontin ; Phosphoproteins - analysis ; Phosphoproteins - blood ; Sialoglycoproteins - analysis ; Sialoglycoproteins - blood ; Synovial Fluid - metabolism ; Vascular Endothelial Growth Factor A - analysis ; Vascular Endothelial Growth Factor A - blood</subject><ispartof>British journal of rheumatology, 2004-09, Vol.43 (9), p.1091-1096</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Sep 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-6cf80cf1628ed58402c9321f403d3ed814840e8be3439b4e7030cdb5b1db4b833</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16097647$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15199218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gattorno, M.</creatorcontrib><creatorcontrib>Gregorio, A.</creatorcontrib><creatorcontrib>Ferlito, F.</creatorcontrib><creatorcontrib>Gerloni, V.</creatorcontrib><creatorcontrib>Parafioriti, A.</creatorcontrib><creatorcontrib>Felici, E.</creatorcontrib><creatorcontrib>Sala, E.</creatorcontrib><creatorcontrib>Gambini, C.</creatorcontrib><creatorcontrib>Picco, P.</creatorcontrib><creatorcontrib>Martini, A.</creatorcontrib><title>Synovial expression of osteopontin correlates with angiogenesis in juvenile idiopathic arthritis</title><title>British journal of rheumatology</title><addtitle>Rheumatology</addtitle><description>Objective. To evaluate the synovial expression of osteopontin (OPN) and its possible correlation with the degree of synovial angiogenesis in human chronic idiopathic arthritis. Methods. Forty-five patients with active juvenile idiopathic arthritis (JIA) were studied. All patients underwent SF aspiration before steroid injection. A paired plasma sample was collected from 22 JIA patients. Plasma from 15 age-matched healthy subjects was used as control. Plasma and SF were tested by ELISA for OPN and vascular endothelial growth factor (VEGF). Synovial tissue was obtained at synovectomy from 10 JIA patients. Immunohistochemistry was performed according to a standard technique with anti-OPN, anti-CD68, anti-CD31 anti-VEGF and anti-αvβ3 antibodies. Results. OPN levels were significantly higher in SF than in paired plasma samples (P<0.001). The same pattern was observed for VEGF (P<0.001). A positive correlation between OPN and VEGF concentrations was found in SF (r = 0.6, P = 0.001). In synovial tissue, OPN was expressed at the level of the lining and sublining layers with a distribution similar to that observed for VEGF. OPN expression in the lining layer correlated with the number of vessels present in the areas underlying the sublining layer. Conclusions. Synovial expression of OPN correlates with parameters of angiogenesis in JIA. These data support, in human disease, the possible role of OPN in the vascularization of inflamed synovial tissue, as previously shown in OPN-deficient animal models of arthritis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Angiogenesis</subject><subject>Antigens, CD - analysis</subject><subject>Arthritis, Juvenile - metabolism</subject><subject>Arthritis, Juvenile - physiopathology</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Diseases of the osteoarticular system</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Inflammatory joint diseases</subject><subject>Integrins</subject><subject>Integrins - analysis</subject><subject>Joints - blood supply</subject><subject>Juvenile idiopathic arthritis</subject><subject>Medical sciences</subject><subject>Neovascularization, Pathologic - physiopathology</subject><subject>Osteopontin</subject><subject>Phosphoproteins - analysis</subject><subject>Phosphoproteins - blood</subject><subject>Sialoglycoproteins - analysis</subject><subject>Sialoglycoproteins - blood</subject><subject>Synovial Fluid - metabolism</subject><subject>Vascular Endothelial Growth Factor A - analysis</subject><subject>Vascular Endothelial Growth Factor A - blood</subject><issn>1462-0324</issn><issn>1460-2172</issn><issn>1462-0332</issn><issn>1460-2172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0d9rFDEQB_BFLLZW_wJBFsG-rc3vTR6laM-jULAWii8xm529zXUvWZNs2_vv3XJHK774NGHmMwPhWxTvMPqEkaKnsYdpY3IYwmp7egs94ehFcYSZIBWilLx8ehN2WLxOaY0Q4pjKV8Uh5lgpguVR8etq68OdM0MJD2OElFzwZejKkDKEMfjsfGlDjDCYDKm8d7kvjV-5sAIPyaVynq-nO_BugNK1Lowm986WJuY-uuzSm-KgM0OCt_t6XFx__fLjbFFdXJ5_O_t8UVmmZK6E7SSyHRZEQsslQ8QqSnDHEG0ptBKzuQeyAcqoahjUiCLbNrzBbcMaSelxcbK7O8bwe4KU9cYlC8NgPIQpaSFqxRVB_4W4lpJg9Qg__APXYYp-_oTGiotaCi5nRHfIxpBShE6P0W1M3GqM9GNM-u-Y9C6meev9_vTUbKB93tnnMoOPe2CSNUMXjbcuPTuBVC1YPbtq59yc18PT3MRbLWpac724-akXy-UVxsvv-pz-AbAYsLg</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Gattorno, M.</creator><creator>Gregorio, A.</creator><creator>Ferlito, F.</creator><creator>Gerloni, V.</creator><creator>Parafioriti, A.</creator><creator>Felici, E.</creator><creator>Sala, E.</creator><creator>Gambini, C.</creator><creator>Picco, P.</creator><creator>Martini, A.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>Synovial expression of osteopontin correlates with angiogenesis in juvenile idiopathic arthritis</title><author>Gattorno, M. ; Gregorio, A. ; Ferlito, F. ; Gerloni, V. ; Parafioriti, A. ; Felici, E. ; Sala, E. ; Gambini, C. ; Picco, P. ; Martini, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-6cf80cf1628ed58402c9321f403d3ed814840e8be3439b4e7030cdb5b1db4b833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Angiogenesis</topic><topic>Antigens, CD - analysis</topic><topic>Arthritis, Juvenile - metabolism</topic><topic>Arthritis, Juvenile - physiopathology</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Diseases of the osteoarticular system</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Inflammatory joint diseases</topic><topic>Integrins</topic><topic>Integrins - analysis</topic><topic>Joints - blood supply</topic><topic>Juvenile idiopathic arthritis</topic><topic>Medical sciences</topic><topic>Neovascularization, Pathologic - physiopathology</topic><topic>Osteopontin</topic><topic>Phosphoproteins - analysis</topic><topic>Phosphoproteins - blood</topic><topic>Sialoglycoproteins - analysis</topic><topic>Sialoglycoproteins - blood</topic><topic>Synovial Fluid - metabolism</topic><topic>Vascular Endothelial Growth Factor A - analysis</topic><topic>Vascular Endothelial Growth Factor A - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gattorno, M.</creatorcontrib><creatorcontrib>Gregorio, A.</creatorcontrib><creatorcontrib>Ferlito, F.</creatorcontrib><creatorcontrib>Gerloni, V.</creatorcontrib><creatorcontrib>Parafioriti, A.</creatorcontrib><creatorcontrib>Felici, E.</creatorcontrib><creatorcontrib>Sala, E.</creatorcontrib><creatorcontrib>Gambini, C.</creatorcontrib><creatorcontrib>Picco, P.</creatorcontrib><creatorcontrib>Martini, A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of rheumatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gattorno, M.</au><au>Gregorio, A.</au><au>Ferlito, F.</au><au>Gerloni, V.</au><au>Parafioriti, A.</au><au>Felici, E.</au><au>Sala, E.</au><au>Gambini, C.</au><au>Picco, P.</au><au>Martini, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synovial expression of osteopontin correlates with angiogenesis in juvenile idiopathic arthritis</atitle><jtitle>British journal of rheumatology</jtitle><addtitle>Rheumatology</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>43</volume><issue>9</issue><spage>1091</spage><epage>1096</epage><pages>1091-1096</pages><issn>1462-0324</issn><issn>1460-2172</issn><eissn>1462-0332</eissn><eissn>1460-2172</eissn><coden>BJRHDF</coden><abstract>Objective. To evaluate the synovial expression of osteopontin (OPN) and its possible correlation with the degree of synovial angiogenesis in human chronic idiopathic arthritis. Methods. Forty-five patients with active juvenile idiopathic arthritis (JIA) were studied. All patients underwent SF aspiration before steroid injection. A paired plasma sample was collected from 22 JIA patients. Plasma from 15 age-matched healthy subjects was used as control. Plasma and SF were tested by ELISA for OPN and vascular endothelial growth factor (VEGF). Synovial tissue was obtained at synovectomy from 10 JIA patients. Immunohistochemistry was performed according to a standard technique with anti-OPN, anti-CD68, anti-CD31 anti-VEGF and anti-αvβ3 antibodies. Results. OPN levels were significantly higher in SF than in paired plasma samples (P<0.001). The same pattern was observed for VEGF (P<0.001). A positive correlation between OPN and VEGF concentrations was found in SF (r = 0.6, P = 0.001). In synovial tissue, OPN was expressed at the level of the lining and sublining layers with a distribution similar to that observed for VEGF. OPN expression in the lining layer correlated with the number of vessels present in the areas underlying the sublining layer. Conclusions. Synovial expression of OPN correlates with parameters of angiogenesis in JIA. These data support, in human disease, the possible role of OPN in the vascularization of inflamed synovial tissue, as previously shown in OPN-deficient animal models of arthritis.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15199218</pmid><doi>10.1093/rheumatology/keh250</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1462-0324 |
| ispartof | British journal of rheumatology, 2004-09, Vol.43 (9), p.1091-1096 |
| issn | 1462-0324 1460-2172 1462-0332 1460-2172 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66795920 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Alma/SFX Local Collection |
| subjects | Adolescent Adult Angiogenesis Antigens, CD - analysis Arthritis, Juvenile - metabolism Arthritis, Juvenile - physiopathology Biological and medical sciences Child Diseases of the osteoarticular system Female Humans Immunohistochemistry - methods Inflammatory joint diseases Integrins Integrins - analysis Joints - blood supply Juvenile idiopathic arthritis Medical sciences Neovascularization, Pathologic - physiopathology Osteopontin Phosphoproteins - analysis Phosphoproteins - blood Sialoglycoproteins - analysis Sialoglycoproteins - blood Synovial Fluid - metabolism Vascular Endothelial Growth Factor A - analysis Vascular Endothelial Growth Factor A - blood |
| title | Synovial expression of osteopontin correlates with angiogenesis in juvenile idiopathic arthritis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T20%3A07%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Synovial%20expression%20of%20osteopontin%20correlates%20with%20angiogenesis%20in%20juvenile%20idiopathic%20arthritis&rft.jtitle=British%20journal%20of%20rheumatology&rft.au=Gattorno,%20M.&rft.date=2004-09-01&rft.volume=43&rft.issue=9&rft.spage=1091&rft.epage=1096&rft.pages=1091-1096&rft.issn=1462-0324&rft.eissn=1462-0332&rft.coden=BJRHDF&rft_id=info:doi/10.1093/rheumatology/keh250&rft_dat=%3Cproquest_cross%3E17882190%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=195678658&rft_id=info:pmid/15199218&rfr_iscdi=true |