Cryo-EM Visualization of a Viral Internal Ribosome Entry Site Bound to Human Ribosomes: The IRES Functions as an RNA-Based Translation Factor
Internal initiation of protein synthesis in eukaryotes is accomplished by recruitment of ribosomes to structured internal ribosome entry sites (IRESs), which are located in certain viral and cellular messenger RNAs. An IRES element in cricket paralysis virus (CrPV) can directly assemble 80S ribosome...
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| Veröffentlicht in: | Cell 2004-08, Vol.118 (4), p.465-475 |
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| creator | Spahn, Christian M.T. Jan, Eric Mulder, Anke Grassucci, Robert A. Sarnow, Peter Frank, Joachim |
| description | Internal initiation of protein synthesis in eukaryotes is accomplished by recruitment of ribosomes to structured internal ribosome entry sites (IRESs), which are located in certain viral and cellular messenger RNAs. An IRES element in cricket paralysis virus (CrPV) can directly assemble 80S ribosomes in the absence of canonical initiation factors and initiator tRNA. Here we present cryo-EM structures of the CrPV IRES bound to the human ribosomal 40S subunit and to the 80S ribosome. The CrPV IRES adopts a defined, elongate structure within the ribosomal intersubunit space and forms specific contacts with components of the ribosomal A, P, and E sites. Conformational changes in the ribosome as well as within the IRES itself show that CrPV IRES actively manipulates the ribosome. CrPV-like IRES elements seem to act as RNA-based translation factors. |
| doi_str_mv | 10.1016/j.cell.2004.08.001 |
| format | Article |
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An IRES element in cricket paralysis virus (CrPV) can directly assemble 80S ribosomes in the absence of canonical initiation factors and initiator tRNA. Here we present cryo-EM structures of the CrPV IRES bound to the human ribosomal 40S subunit and to the 80S ribosome. The CrPV IRES adopts a defined, elongate structure within the ribosomal intersubunit space and forms specific contacts with components of the ribosomal A, P, and E sites. Conformational changes in the ribosome as well as within the IRES itself show that CrPV IRES actively manipulates the ribosome. CrPV-like IRES elements seem to act as RNA-based translation factors.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15315759</pmid><doi>10.1016/j.cell.2004.08.001</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Conserved Sequence Cricket paralysis virus Cryoelectron Microscopy - methods Escherichia coli - metabolism HeLa Cells Humans Models, Molecular Protein Binding Protein Biosynthesis Protein Conformation Ribosomes - chemistry Ribosomes - metabolism RNA, Messenger - metabolism RNA, Transfer - metabolism RNA, Viral - metabolism |
| title | Cryo-EM Visualization of a Viral Internal Ribosome Entry Site Bound to Human Ribosomes: The IRES Functions as an RNA-Based Translation Factor |
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