Generation of anti-idiotypic antibodies that mimic HBsAg and vaccination against hepatitis B virus
The advent of hybridoma technology has opened up a new avenue in vaccine development, and antigen-mimicking properties of anti-idiotypic antibodies have provided promising alternatives in the generation of experimental anti-idiotypic vaccines. In the present study, mice were immunized with anti-hepa...
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| Veröffentlicht in: | Hybridoma and hybridomics 2004-06, Vol.23 (3), p.192-197 |
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| container_title | Hybridoma and hybridomics |
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| creator | Yildirim, Tuba Başalp, Aynur Yücel, Fatima Manav, Ali Ihsan Sezen, I Yavuz |
| description | The advent of hybridoma technology has opened up a new avenue in vaccine development, and antigen-mimicking properties of anti-idiotypic antibodies have provided promising alternatives in the generation of experimental anti-idiotypic vaccines. In the present study, mice were immunized with anti-hepatitis B virus (HBV) mouse monoclonal and anti-HBV goat polyclonal antibody to produce anti-idiotypic antibodies. Two mouse monoclonal antibodies (6C9, 6H9) were obtained from the fusions, and the immunogenic properties and specificity of antibodies were analyzed. BALB/c mice were immunized with varying concentrations of anti-idiotypic antibodies (25, 50, 75, and 100 micrograms of anti-Id), and it was shown that anti-idiotypic antibodies generated hepatitis B surface antigen (HBsAg), as well as a BSA-specific antibody response. A simple method for the purification of monoclonal antibodies by dialyzing antibody against water has also been reported. |
| doi_str_mv | 10.1089/1536859041224307 |
| format | Article |
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In the present study, mice were immunized with anti-hepatitis B virus (HBV) mouse monoclonal and anti-HBV goat polyclonal antibody to produce anti-idiotypic antibodies. Two mouse monoclonal antibodies (6C9, 6H9) were obtained from the fusions, and the immunogenic properties and specificity of antibodies were analyzed. BALB/c mice were immunized with varying concentrations of anti-idiotypic antibodies (25, 50, 75, and 100 micrograms of anti-Id), and it was shown that anti-idiotypic antibodies generated hepatitis B surface antigen (HBsAg), as well as a BSA-specific antibody response. A simple method for the purification of monoclonal antibodies by dialyzing antibody against water has also been reported.</description><subject>Animals</subject><subject>Antibodies, Anti-Idiotypic - biosynthesis</subject><subject>Antibodies, Anti-Idiotypic - immunology</subject><subject>Antibodies, Anti-Idiotypic - isolation & purification</subject><subject>Antibodies, Anti-Idiotypic - metabolism</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - isolation & purification</subject><subject>Antibodies, Monoclonal - metabolism</subject><subject>Antibody Specificity</subject><subject>Hepatitis B Surface Antigens - immunology</subject><subject>Hepatitis B Vaccines - immunology</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - immunology</subject><subject>Hybridomas - secretion</subject><subject>Immunoglobulin M - biosynthesis</subject><subject>Immunoglobulin M - immunology</subject><subject>Immunoglobulin M - isolation & purification</subject><subject>Immunoglobulin M - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Vaccination</subject><issn>1536-8599</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1PwzAQxT2AaCnsTMgTW-D8UccZ2wpapEosMEeOc2mNmg9ip1L_e1xaCYmF6XS_997p9Ai5Y_DIQGdPbCqUnmYgGedSQHpBxkeURJaNyLX3nwBMacWvyCgKjAvGxqRYYoO9Ca5taFtR0wSXuNK14dA5-7MWbenQ07A1gdaujnQ197NN1Eq6N9a65pQ2G-MaH-gWuwiC83RO964f_A25rMzO4-15TsjHy_P7YpWs35avi9k6sYKrkMSfMskVpFwzDjqtNBcildpIba1MGZfIUUqIhsIWWBk7rSAttcGsEBUWYkIeTne7vv0a0Ie8dt7ibmcabAefK5VmsQH-r5FpAAVKRiOcjLZvve-xyrve1aY_5AzyY-n539Jj5P58eyhqLH8D58bFN8MFffY</recordid><startdate>200406</startdate><enddate>200406</enddate><creator>Yildirim, Tuba</creator><creator>Başalp, Aynur</creator><creator>Yücel, Fatima</creator><creator>Manav, Ali Ihsan</creator><creator>Sezen, I Yavuz</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200406</creationdate><title>Generation of anti-idiotypic antibodies that mimic HBsAg and vaccination against hepatitis B virus</title><author>Yildirim, Tuba ; Başalp, Aynur ; Yücel, Fatima ; Manav, Ali Ihsan ; Sezen, I Yavuz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-1539426072812087f8233748a48cc47124e2e440072bcbefac5f07d8ae9b3feb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antibodies, Anti-Idiotypic - biosynthesis</topic><topic>Antibodies, Anti-Idiotypic - immunology</topic><topic>Antibodies, Anti-Idiotypic - isolation & purification</topic><topic>Antibodies, Anti-Idiotypic - metabolism</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Monoclonal - isolation & purification</topic><topic>Antibodies, Monoclonal - metabolism</topic><topic>Antibody Specificity</topic><topic>Hepatitis B Surface Antigens - immunology</topic><topic>Hepatitis B Vaccines - immunology</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus - immunology</topic><topic>Hybridomas - secretion</topic><topic>Immunoglobulin M - biosynthesis</topic><topic>Immunoglobulin M - immunology</topic><topic>Immunoglobulin M - isolation & purification</topic><topic>Immunoglobulin M - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Vaccination</topic><toplevel>online_resources</toplevel><creatorcontrib>Yildirim, Tuba</creatorcontrib><creatorcontrib>Başalp, Aynur</creatorcontrib><creatorcontrib>Yücel, Fatima</creatorcontrib><creatorcontrib>Manav, Ali Ihsan</creatorcontrib><creatorcontrib>Sezen, I Yavuz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Hybridoma and hybridomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yildirim, Tuba</au><au>Başalp, Aynur</au><au>Yücel, Fatima</au><au>Manav, Ali Ihsan</au><au>Sezen, I Yavuz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of anti-idiotypic antibodies that mimic HBsAg and vaccination against hepatitis B virus</atitle><jtitle>Hybridoma and hybridomics</jtitle><addtitle>Hybrid Hybridomics</addtitle><date>2004-06</date><risdate>2004</risdate><volume>23</volume><issue>3</issue><spage>192</spage><epage>197</epage><pages>192-197</pages><issn>1536-8599</issn><abstract>The advent of hybridoma technology has opened up a new avenue in vaccine development, and antigen-mimicking properties of anti-idiotypic antibodies have provided promising alternatives in the generation of experimental anti-idiotypic vaccines. 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| ispartof | Hybridoma and hybridomics, 2004-06, Vol.23 (3), p.192-197 |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Animals Antibodies, Anti-Idiotypic - biosynthesis Antibodies, Anti-Idiotypic - immunology Antibodies, Anti-Idiotypic - isolation & purification Antibodies, Anti-Idiotypic - metabolism Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Antibodies, Monoclonal - metabolism Antibody Specificity Hepatitis B Surface Antigens - immunology Hepatitis B Vaccines - immunology Hepatitis B virus Hepatitis B virus - immunology Hybridomas - secretion Immunoglobulin M - biosynthesis Immunoglobulin M - immunology Immunoglobulin M - isolation & purification Immunoglobulin M - metabolism Mice Mice, Inbred BALB C Vaccination |
| title | Generation of anti-idiotypic antibodies that mimic HBsAg and vaccination against hepatitis B virus |
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