A drop of intracellular pH stimulates citric acid accumulation by some strains of Aspergillus niger
By comparing kinetic parameters of plasma membrane proton pumps from two Aspergillus niger strains, significant differences in specific activities were observed. In low citric acid producing A158 strain the H +-ATPase activity was about four-fold higher than in a high yielding A60 strain. Previously...
Gespeichert in:
| Veröffentlicht in: | Journal of biotechnology 2004-09, Vol.112 (3), p.289-297 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 297 |
|---|---|
| container_issue | 3 |
| container_start_page | 289 |
| container_title | Journal of biotechnology |
| container_volume | 112 |
| creator | Jernejc, Katarina Legiša, Matic |
| description | By comparing kinetic parameters of plasma membrane proton pumps from two
Aspergillus niger strains, significant differences in specific activities were observed. In low citric acid producing A158 strain the H
+-ATPase activity was about four-fold higher than in a high yielding A60 strain. Previously pH homeostasis was reported in A158 strain while in A60 strain spontaneous drop of intracellular pH was observed. During the growth in the medium with ammonium ions more rapid drop of extracellular pH was recorded with A158 strain and not so fast proton accumulation in the medium with A60 strain, indicating that proton pumps from later strain perhaps can not extrude all the protons that are released in the cytosol after the assimilation of ammonium ions. Vanadium ions were found to be potent inhibitors of both H
+-ATPases. By adding sodium vanadate in millimolar concentrations to the chemically defined medium that induces citric acid accumulation by
A. niger, reduced pH
i and increased rate of acid production was observed in A158 strain while in A60 strain intracellular pH decreased below 6.5 and concomitantly citric acid overflow was suppressed. The presented results suggest that one of the mechanisms stimulating citric acid accumulation by
A. niger could be also a slight cytoplasmic acidification. |
| doi_str_mv | 10.1016/j.jbiotec.2004.05.002 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66789881</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168165604002445</els_id><sourcerecordid>18029312</sourcerecordid><originalsourceid>FETCH-LOGICAL-c446t-14d05f4d039c768e6ca1a782b12e02d66122109a659f197d714bb1b80f8d3e5f3</originalsourceid><addsrcrecordid>eNqFkcFu1DAQhi0EotuFRwB8obeEGSdxnBNaVUCRKnGAni3Hmay8SuJgZyv17XHYSD32MjOyvvlnPD9jHxByBJRfTvmpdX4hmwuAMocqBxCv2A5VXWSlksVrtkucylBW8opdx3iCBDYVvmVXWBVYAMgdswfeBT9z33M3LcFYGobzYAKf73hc3JjqhSK3bgnOcmNdl4I9_393fuLtE49-pMQG46a46hziTOHokk7kkztSeMfe9GaI9H7Le_bw_duf27vs_tePn7eH-8yWpVwyLDuo-hSKxtZSkbQGTa1Ei4JAdFKiEAiNkVXTY1N3NZZti62CXnUFVX2xZzcX3Tn4v2eKix5dXD9kJvLnqKWsVaMUvgiiAtEUKBJYXUAbfIyBej0HN5rwpBH0aoM-6c0GvdqgodLJhtT3cRtwbkfqnru2uyfg8waYaM3QBzNZF585mURU2mHPPl243nhtjiExD78FrCJN2YAoE_H1QlC67KOjoKN1NFnqXCC76M67F5b9B2lxsYA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18029312</pqid></control><display><type>article</type><title>A drop of intracellular pH stimulates citric acid accumulation by some strains of Aspergillus niger</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Jernejc, Katarina ; Legiša, Matic</creator><creatorcontrib>Jernejc, Katarina ; Legiša, Matic</creatorcontrib><description>By comparing kinetic parameters of plasma membrane proton pumps from two
Aspergillus niger strains, significant differences in specific activities were observed. In low citric acid producing A158 strain the H
+-ATPase activity was about four-fold higher than in a high yielding A60 strain. Previously pH homeostasis was reported in A158 strain while in A60 strain spontaneous drop of intracellular pH was observed. During the growth in the medium with ammonium ions more rapid drop of extracellular pH was recorded with A158 strain and not so fast proton accumulation in the medium with A60 strain, indicating that proton pumps from later strain perhaps can not extrude all the protons that are released in the cytosol after the assimilation of ammonium ions. Vanadium ions were found to be potent inhibitors of both H
+-ATPases. By adding sodium vanadate in millimolar concentrations to the chemically defined medium that induces citric acid accumulation by
A. niger, reduced pH
i and increased rate of acid production was observed in A158 strain while in A60 strain intracellular pH decreased below 6.5 and concomitantly citric acid overflow was suppressed. The presented results suggest that one of the mechanisms stimulating citric acid accumulation by
A. niger could be also a slight cytoplasmic acidification.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/j.jbiotec.2004.05.002</identifier><identifier>PMID: 15313006</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Aspergillus niger ; Aspergillus niger - classification ; Aspergillus niger - drug effects ; Aspergillus niger - growth & development ; Aspergillus niger - metabolism ; Biological and medical sciences ; Biotechnology ; Cell Culture Techniques - methods ; Citric acid ; Citric Acid - chemistry ; Citric Acid - metabolism ; Dose-Response Relationship, Drug ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; H +-ATPases ; H-transporting ATPase ; Hydrogen-Ion Concentration - drug effects ; Intracellular Fluid - chemistry ; Intracellular Fluid - drug effects ; Intracellular Fluid - metabolism ; Intracellular pH ; Nitrogen source ; proton pump ; Species Specificity ; Vanadates - pharmacology ; Vanadium ions</subject><ispartof>Journal of biotechnology, 2004-09, Vol.112 (3), p.289-297</ispartof><rights>2004 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-14d05f4d039c768e6ca1a782b12e02d66122109a659f197d714bb1b80f8d3e5f3</citedby><cites>FETCH-LOGICAL-c446t-14d05f4d039c768e6ca1a782b12e02d66122109a659f197d714bb1b80f8d3e5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168165604002445$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16023829$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15313006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jernejc, Katarina</creatorcontrib><creatorcontrib>Legiša, Matic</creatorcontrib><title>A drop of intracellular pH stimulates citric acid accumulation by some strains of Aspergillus niger</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>By comparing kinetic parameters of plasma membrane proton pumps from two
Aspergillus niger strains, significant differences in specific activities were observed. In low citric acid producing A158 strain the H
+-ATPase activity was about four-fold higher than in a high yielding A60 strain. Previously pH homeostasis was reported in A158 strain while in A60 strain spontaneous drop of intracellular pH was observed. During the growth in the medium with ammonium ions more rapid drop of extracellular pH was recorded with A158 strain and not so fast proton accumulation in the medium with A60 strain, indicating that proton pumps from later strain perhaps can not extrude all the protons that are released in the cytosol after the assimilation of ammonium ions. Vanadium ions were found to be potent inhibitors of both H
+-ATPases. By adding sodium vanadate in millimolar concentrations to the chemically defined medium that induces citric acid accumulation by
A. niger, reduced pH
i and increased rate of acid production was observed in A158 strain while in A60 strain intracellular pH decreased below 6.5 and concomitantly citric acid overflow was suppressed. The presented results suggest that one of the mechanisms stimulating citric acid accumulation by
A. niger could be also a slight cytoplasmic acidification.</description><subject>Aspergillus niger</subject><subject>Aspergillus niger - classification</subject><subject>Aspergillus niger - drug effects</subject><subject>Aspergillus niger - growth & development</subject><subject>Aspergillus niger - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Citric acid</subject><subject>Citric Acid - chemistry</subject><subject>Citric Acid - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>H +-ATPases</subject><subject>H-transporting ATPase</subject><subject>Hydrogen-Ion Concentration - drug effects</subject><subject>Intracellular Fluid - chemistry</subject><subject>Intracellular Fluid - drug effects</subject><subject>Intracellular Fluid - metabolism</subject><subject>Intracellular pH</subject><subject>Nitrogen source</subject><subject>proton pump</subject><subject>Species Specificity</subject><subject>Vanadates - pharmacology</subject><subject>Vanadium ions</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EotuFRwB8obeEGSdxnBNaVUCRKnGAni3Hmay8SuJgZyv17XHYSD32MjOyvvlnPD9jHxByBJRfTvmpdX4hmwuAMocqBxCv2A5VXWSlksVrtkucylBW8opdx3iCBDYVvmVXWBVYAMgdswfeBT9z33M3LcFYGobzYAKf73hc3JjqhSK3bgnOcmNdl4I9_393fuLtE49-pMQG46a46hziTOHokk7kkztSeMfe9GaI9H7Le_bw_duf27vs_tePn7eH-8yWpVwyLDuo-hSKxtZSkbQGTa1Ei4JAdFKiEAiNkVXTY1N3NZZti62CXnUFVX2xZzcX3Tn4v2eKix5dXD9kJvLnqKWsVaMUvgiiAtEUKBJYXUAbfIyBej0HN5rwpBH0aoM-6c0GvdqgodLJhtT3cRtwbkfqnru2uyfg8waYaM3QBzNZF585mURU2mHPPl243nhtjiExD78FrCJN2YAoE_H1QlC67KOjoKN1NFnqXCC76M67F5b9B2lxsYA</recordid><startdate>20040909</startdate><enddate>20040909</enddate><creator>Jernejc, Katarina</creator><creator>Legiša, Matic</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040909</creationdate><title>A drop of intracellular pH stimulates citric acid accumulation by some strains of Aspergillus niger</title><author>Jernejc, Katarina ; Legiša, Matic</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-14d05f4d039c768e6ca1a782b12e02d66122109a659f197d714bb1b80f8d3e5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aspergillus niger</topic><topic>Aspergillus niger - classification</topic><topic>Aspergillus niger - drug effects</topic><topic>Aspergillus niger - growth & development</topic><topic>Aspergillus niger - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - methods</topic><topic>Citric acid</topic><topic>Citric Acid - chemistry</topic><topic>Citric Acid - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>H +-ATPases</topic><topic>H-transporting ATPase</topic><topic>Hydrogen-Ion Concentration - drug effects</topic><topic>Intracellular Fluid - chemistry</topic><topic>Intracellular Fluid - drug effects</topic><topic>Intracellular Fluid - metabolism</topic><topic>Intracellular pH</topic><topic>Nitrogen source</topic><topic>proton pump</topic><topic>Species Specificity</topic><topic>Vanadates - pharmacology</topic><topic>Vanadium ions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jernejc, Katarina</creatorcontrib><creatorcontrib>Legiša, Matic</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jernejc, Katarina</au><au>Legiša, Matic</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A drop of intracellular pH stimulates citric acid accumulation by some strains of Aspergillus niger</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2004-09-09</date><risdate>2004</risdate><volume>112</volume><issue>3</issue><spage>289</spage><epage>297</epage><pages>289-297</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>By comparing kinetic parameters of plasma membrane proton pumps from two
Aspergillus niger strains, significant differences in specific activities were observed. In low citric acid producing A158 strain the H
+-ATPase activity was about four-fold higher than in a high yielding A60 strain. Previously pH homeostasis was reported in A158 strain while in A60 strain spontaneous drop of intracellular pH was observed. During the growth in the medium with ammonium ions more rapid drop of extracellular pH was recorded with A158 strain and not so fast proton accumulation in the medium with A60 strain, indicating that proton pumps from later strain perhaps can not extrude all the protons that are released in the cytosol after the assimilation of ammonium ions. Vanadium ions were found to be potent inhibitors of both H
+-ATPases. By adding sodium vanadate in millimolar concentrations to the chemically defined medium that induces citric acid accumulation by
A. niger, reduced pH
i and increased rate of acid production was observed in A158 strain while in A60 strain intracellular pH decreased below 6.5 and concomitantly citric acid overflow was suppressed. The presented results suggest that one of the mechanisms stimulating citric acid accumulation by
A. niger could be also a slight cytoplasmic acidification.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>15313006</pmid><doi>10.1016/j.jbiotec.2004.05.002</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1656 |
| ispartof | Journal of biotechnology, 2004-09, Vol.112 (3), p.289-297 |
| issn | 0168-1656 1873-4863 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66789881 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aspergillus niger Aspergillus niger - classification Aspergillus niger - drug effects Aspergillus niger - growth & development Aspergillus niger - metabolism Biological and medical sciences Biotechnology Cell Culture Techniques - methods Citric acid Citric Acid - chemistry Citric Acid - metabolism Dose-Response Relationship, Drug enzyme activity Fundamental and applied biological sciences. Psychology H +-ATPases H-transporting ATPase Hydrogen-Ion Concentration - drug effects Intracellular Fluid - chemistry Intracellular Fluid - drug effects Intracellular Fluid - metabolism Intracellular pH Nitrogen source proton pump Species Specificity Vanadates - pharmacology Vanadium ions |
| title | A drop of intracellular pH stimulates citric acid accumulation by some strains of Aspergillus niger |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T17%3A55%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20drop%20of%20intracellular%20pH%20stimulates%20citric%20acid%20accumulation%20by%20some%20strains%20of%20Aspergillus%20niger&rft.jtitle=Journal%20of%20biotechnology&rft.au=Jernejc,%20Katarina&rft.date=2004-09-09&rft.volume=112&rft.issue=3&rft.spage=289&rft.epage=297&rft.pages=289-297&rft.issn=0168-1656&rft.eissn=1873-4863&rft.coden=JBITD4&rft_id=info:doi/10.1016/j.jbiotec.2004.05.002&rft_dat=%3Cproquest_cross%3E18029312%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18029312&rft_id=info:pmid/15313006&rft_els_id=S0168165604002445&rfr_iscdi=true |