Biochemical characterization of a thermostable cysteine synthase from Geobacillus stearothermophilus V

The cysK gene encoding a cysteine synthase of Geobacillus stearothermophilus V was overexpressed in E. coli and the recombinant protein was purified and characterized. The enzyme is a thermostable homodimer (32 kDa/monomer) belonging to the β family of pyridoxal phosphate (PLP)-dependent enzymes. UV...

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Veröffentlicht in:	Biochimie 2004-07, Vol.86 (7), p.481-485
Hauptverfasser:	Saavedra, C.P., Encinas, M.V., Araya, M.A., Pérez, J.M., Tantaleán, J.C., Fuentes, D.E., Calderón, I.L., Pichuantes, S.E., Vásquez, C.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillaceae - enzymology Cysteine biosynthesis Cysteine synthase Cysteine Synthase - chemistry Cysteine Synthase - isolation & purification Cysteine Synthase - metabolism Enzyme Stability Kinetics Spectrometry, Fluorescence Spectrophotometry Thermodynamics α-Aminoacrylate, Geobacillus stearothermophilus
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