Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro
The detection of galectin-1 (gal-1) in pig granulosa cell lysates by immunoblotting and its cytosolic as well as membrane-associated localization prompted us to study its effects on cell proliferation and regulation of progesterone synthesis. The lectin stimulated the proliferation of granulosa cell...
Gespeichert in:
| Veröffentlicht in: | Glycobiology (Oxford) 2004-10, Vol.14 (10), p.871-881 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 881 |
|---|---|
| container_issue | 10 |
| container_start_page | 871 |
| container_title | Glycobiology (Oxford) |
| container_volume | 14 |
| creator | Walzel, Hermann Brock, Josef Pöhland, Ralf Vanselow, Jens Tomek, Wolfgang Schneider, Falk Tiemann, Ute |
| description | The detection of galectin-1 (gal-1) in pig granulosa cell lysates by immunoblotting and its cytosolic as well as membrane-associated localization prompted us to study its effects on cell proliferation and regulation of progesterone synthesis. The lectin stimulated the proliferation of granulosa cells from pig ovaries cultured in serum-free medium. Gal-1 inhibited the FSH-stimulated progesterone synthesis of granulosa cells. This inhibitory effect was strongly reduced by the disaccharidic competitor lactose at 30 mM. The absence of inhibitory effects on dibutyryl-cAMP (db-cAMP), forskolin, and pregnenolone-enhanced cellular progesterone synthesis suggests that gal-1interferes with the receptor-dependent mechanism of FSH-stimulated progesterone production. In FSH-stimulated granulosa cells, western blot analysis revealed the gal-1-mediated suppression of the cytochrome P450–dependent cholesterol side chain cleavage enzyme (P450SCC) that catalyzes the conversion of cholesterol to pregnenolone. In the presence of 30 mM lactose, the gal-1-reduced P450SCC expression was prevented. Strongly reduced mRNA levels were recorded for P450SCC and 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD) when FSH-stimulated granulosa cells were cultured in the presence of gal-1. We conclude that gal-1 exerts its inhibitory effect on steroidogenic activity of granulosa cells by interfering the hormone–receptor interaction resulting in decreased responses to FSH stimulation. |
| doi_str_mv | 10.1093/glycob/cwh101 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66783672</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>729622281</sourcerecordid><originalsourceid>FETCH-LOGICAL-c393t-8ce29872121208b89997cd517bc5e2030435bce0bc43de842596d467621422be3</originalsourceid><addsrcrecordid>eNpdkUtLxDAUhYMoOo4u3Upw4a5O3m2W4hsUQRTETUjTtEY7zZi0Pv69qTMoSBa5l_NxONwDwB5GRxhJOmvaL-PLmfl4xgivgQlmAmWEEboOJkhymQnBiy2wHeMLQljggm-CLcyxoIKICXg9q2tr-gh9DRvdptF1GYa-g8E2Q6t7l8akLYJvbOxt8J0dl2owP5LrYBN0N7Q-amhs20ZYBz-HC9dA_66Ds3Fk3l0f_A7YqHUb7e7qn4KH87P7k8vs-vbi6uT4OjNU0j4rjCWyyAlODxVlIaXMTcVxXhpuCaKIUV4ai0rDaGULRrgUFRO5IJgRUlo6BYdL35TzbUip1dzFMZvurB-iEiIvqMhJAg_-gS9-CF3KpghGlDGERihbQib4GIOt1SK4uQ5fCiM1VqCWFahlBYnfX5kO5dxWf_Tq5n-GLt3z81fX4VWJnOZcXT4-qTuBbk75DVWMfgMu1JKy</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>210344002</pqid></control><display><type>article</type><title>Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Walzel, Hermann ; Brock, Josef ; Pöhland, Ralf ; Vanselow, Jens ; Tomek, Wolfgang ; Schneider, Falk ; Tiemann, Ute</creator><creatorcontrib>Walzel, Hermann ; Brock, Josef ; Pöhland, Ralf ; Vanselow, Jens ; Tomek, Wolfgang ; Schneider, Falk ; Tiemann, Ute</creatorcontrib><description>The detection of galectin-1 (gal-1) in pig granulosa cell lysates by immunoblotting and its cytosolic as well as membrane-associated localization prompted us to study its effects on cell proliferation and regulation of progesterone synthesis. The lectin stimulated the proliferation of granulosa cells from pig ovaries cultured in serum-free medium. Gal-1 inhibited the FSH-stimulated progesterone synthesis of granulosa cells. This inhibitory effect was strongly reduced by the disaccharidic competitor lactose at 30 mM. The absence of inhibitory effects on dibutyryl-cAMP (db-cAMP), forskolin, and pregnenolone-enhanced cellular progesterone synthesis suggests that gal-1interferes with the receptor-dependent mechanism of FSH-stimulated progesterone production. In FSH-stimulated granulosa cells, western blot analysis revealed the gal-1-mediated suppression of the cytochrome P450–dependent cholesterol side chain cleavage enzyme (P450SCC) that catalyzes the conversion of cholesterol to pregnenolone. In the presence of 30 mM lactose, the gal-1-reduced P450SCC expression was prevented. Strongly reduced mRNA levels were recorded for P450SCC and 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD) when FSH-stimulated granulosa cells were cultured in the presence of gal-1. We conclude that gal-1 exerts its inhibitory effect on steroidogenic activity of granulosa cells by interfering the hormone–receptor interaction resulting in decreased responses to FSH stimulation.</description><identifier>ISSN: 0959-6658</identifier><identifier>ISSN: 1460-2423</identifier><identifier>EISSN: 1460-2423</identifier><identifier>DOI: 10.1093/glycob/cwh101</identifier><identifier>PMID: 15163626</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>3β-HSD ; 3β-hydroxysteroid dehydrogenase ; Animals ; bovine serum albumin ; BSA ; carbohydrate recognition domain ; Cattle ; Cell Proliferation - drug effects ; Colforsin - pharmacology ; CRD ; Cyclic AMP - pharmacology ; Cytochrome P-450 Enzyme Inhibitors ; Cytochrome P-450 Enzyme System - genetics ; cytochrome P450–dependent cholesterol side chain cleavage enzyme ; db-cAMP ; dibutyryl cyclic AMP ; ECL ; EDTA ; enhanced chemiluminescence ; ethylenediaminetetraacetic acid ; FCS ; Female ; fetal calf serum ; FITC ; fluorescein isothiocyanate ; follicle stimulating hormone ; Follicle Stimulating Hormone - antagonists & inhibitors ; FSH ; Galectin 1 - antagonists & inhibitors ; Galectin 1 - metabolism ; Galectin 1 - pharmacology ; galectin-1 ; granulosa cells ; Granulosa Cells - drug effects ; Granulosa Cells - metabolism ; horseradish peroxidase ; HRP ; In Vitro Techniques ; Lactose - analogs & derivatives ; Lactose - pharmacology ; mAb ; monoclonal antibody ; Ovary - cytology ; Ovary - physiology ; P450SCC ; pAb ; PAGE ; PBS ; PCR ; phosphate buffered saline ; pig ovaries ; polyacrylamide gel electrophoresis ; polyclonal antibody ; polymerase chain reaction ; Pregnenolone - pharmacology ; Progesterone - antagonists & inhibitors ; Progesterone - biosynthesis ; Progesterone - metabolism ; progesterone synthesis ; reverse transcription ; RNA, Messenger - antagonists & inhibitors ; RNA, Messenger - genetics ; SDS ; sodium dodecyl sulfate ; Swine ; TBS ; TCM ; tissue culture medium ; Tris-buffered saline</subject><ispartof>Glycobiology (Oxford), 2004-10, Vol.14 (10), p.871-881</ispartof><rights>Copyright Oxford University Press(England) Oct 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-8ce29872121208b89997cd517bc5e2030435bce0bc43de842596d467621422be3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15163626$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Walzel, Hermann</creatorcontrib><creatorcontrib>Brock, Josef</creatorcontrib><creatorcontrib>Pöhland, Ralf</creatorcontrib><creatorcontrib>Vanselow, Jens</creatorcontrib><creatorcontrib>Tomek, Wolfgang</creatorcontrib><creatorcontrib>Schneider, Falk</creatorcontrib><creatorcontrib>Tiemann, Ute</creatorcontrib><title>Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro</title><title>Glycobiology (Oxford)</title><addtitle>Glycobiology</addtitle><description>The detection of galectin-1 (gal-1) in pig granulosa cell lysates by immunoblotting and its cytosolic as well as membrane-associated localization prompted us to study its effects on cell proliferation and regulation of progesterone synthesis. The lectin stimulated the proliferation of granulosa cells from pig ovaries cultured in serum-free medium. Gal-1 inhibited the FSH-stimulated progesterone synthesis of granulosa cells. This inhibitory effect was strongly reduced by the disaccharidic competitor lactose at 30 mM. The absence of inhibitory effects on dibutyryl-cAMP (db-cAMP), forskolin, and pregnenolone-enhanced cellular progesterone synthesis suggests that gal-1interferes with the receptor-dependent mechanism of FSH-stimulated progesterone production. In FSH-stimulated granulosa cells, western blot analysis revealed the gal-1-mediated suppression of the cytochrome P450–dependent cholesterol side chain cleavage enzyme (P450SCC) that catalyzes the conversion of cholesterol to pregnenolone. In the presence of 30 mM lactose, the gal-1-reduced P450SCC expression was prevented. Strongly reduced mRNA levels were recorded for P450SCC and 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD) when FSH-stimulated granulosa cells were cultured in the presence of gal-1. We conclude that gal-1 exerts its inhibitory effect on steroidogenic activity of granulosa cells by interfering the hormone–receptor interaction resulting in decreased responses to FSH stimulation.</description><subject>3β-HSD</subject><subject>3β-hydroxysteroid dehydrogenase</subject><subject>Animals</subject><subject>bovine serum albumin</subject><subject>BSA</subject><subject>carbohydrate recognition domain</subject><subject>Cattle</subject><subject>Cell Proliferation - drug effects</subject><subject>Colforsin - pharmacology</subject><subject>CRD</subject><subject>Cyclic AMP - pharmacology</subject><subject>Cytochrome P-450 Enzyme Inhibitors</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>cytochrome P450–dependent cholesterol side chain cleavage enzyme</subject><subject>db-cAMP</subject><subject>dibutyryl cyclic AMP</subject><subject>ECL</subject><subject>EDTA</subject><subject>enhanced chemiluminescence</subject><subject>ethylenediaminetetraacetic acid</subject><subject>FCS</subject><subject>Female</subject><subject>fetal calf serum</subject><subject>FITC</subject><subject>fluorescein isothiocyanate</subject><subject>follicle stimulating hormone</subject><subject>Follicle Stimulating Hormone - antagonists & inhibitors</subject><subject>FSH</subject><subject>Galectin 1 - antagonists & inhibitors</subject><subject>Galectin 1 - metabolism</subject><subject>Galectin 1 - pharmacology</subject><subject>galectin-1</subject><subject>granulosa cells</subject><subject>Granulosa Cells - drug effects</subject><subject>Granulosa Cells - metabolism</subject><subject>horseradish peroxidase</subject><subject>HRP</subject><subject>In Vitro Techniques</subject><subject>Lactose - analogs & derivatives</subject><subject>Lactose - pharmacology</subject><subject>mAb</subject><subject>monoclonal antibody</subject><subject>Ovary - cytology</subject><subject>Ovary - physiology</subject><subject>P450SCC</subject><subject>pAb</subject><subject>PAGE</subject><subject>PBS</subject><subject>PCR</subject><subject>phosphate buffered saline</subject><subject>pig ovaries</subject><subject>polyacrylamide gel electrophoresis</subject><subject>polyclonal antibody</subject><subject>polymerase chain reaction</subject><subject>Pregnenolone - pharmacology</subject><subject>Progesterone - antagonists & inhibitors</subject><subject>Progesterone - biosynthesis</subject><subject>Progesterone - metabolism</subject><subject>progesterone synthesis</subject><subject>reverse transcription</subject><subject>RNA, Messenger - antagonists & inhibitors</subject><subject>RNA, Messenger - genetics</subject><subject>SDS</subject><subject>sodium dodecyl sulfate</subject><subject>Swine</subject><subject>TBS</subject><subject>TCM</subject><subject>tissue culture medium</subject><subject>Tris-buffered saline</subject><issn>0959-6658</issn><issn>1460-2423</issn><issn>1460-2423</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtLxDAUhYMoOo4u3Upw4a5O3m2W4hsUQRTETUjTtEY7zZi0Pv69qTMoSBa5l_NxONwDwB5GRxhJOmvaL-PLmfl4xgivgQlmAmWEEboOJkhymQnBiy2wHeMLQljggm-CLcyxoIKICXg9q2tr-gh9DRvdptF1GYa-g8E2Q6t7l8akLYJvbOxt8J0dl2owP5LrYBN0N7Q-amhs20ZYBz-HC9dA_66Ds3Fk3l0f_A7YqHUb7e7qn4KH87P7k8vs-vbi6uT4OjNU0j4rjCWyyAlODxVlIaXMTcVxXhpuCaKIUV4ai0rDaGULRrgUFRO5IJgRUlo6BYdL35TzbUip1dzFMZvurB-iEiIvqMhJAg_-gS9-CF3KpghGlDGERihbQib4GIOt1SK4uQ5fCiM1VqCWFahlBYnfX5kO5dxWf_Tq5n-GLt3z81fX4VWJnOZcXT4-qTuBbk75DVWMfgMu1JKy</recordid><startdate>20041001</startdate><enddate>20041001</enddate><creator>Walzel, Hermann</creator><creator>Brock, Josef</creator><creator>Pöhland, Ralf</creator><creator>Vanselow, Jens</creator><creator>Tomek, Wolfgang</creator><creator>Schneider, Falk</creator><creator>Tiemann, Ute</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041001</creationdate><title>Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro</title><author>Walzel, Hermann ; Brock, Josef ; Pöhland, Ralf ; Vanselow, Jens ; Tomek, Wolfgang ; Schneider, Falk ; Tiemann, Ute</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-8ce29872121208b89997cd517bc5e2030435bce0bc43de842596d467621422be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>3β-HSD</topic><topic>3β-hydroxysteroid dehydrogenase</topic><topic>Animals</topic><topic>bovine serum albumin</topic><topic>BSA</topic><topic>carbohydrate recognition domain</topic><topic>Cattle</topic><topic>Cell Proliferation - drug effects</topic><topic>Colforsin - pharmacology</topic><topic>CRD</topic><topic>Cyclic AMP - pharmacology</topic><topic>Cytochrome P-450 Enzyme Inhibitors</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>cytochrome P450–dependent cholesterol side chain cleavage enzyme</topic><topic>db-cAMP</topic><topic>dibutyryl cyclic AMP</topic><topic>ECL</topic><topic>EDTA</topic><topic>enhanced chemiluminescence</topic><topic>ethylenediaminetetraacetic acid</topic><topic>FCS</topic><topic>Female</topic><topic>fetal calf serum</topic><topic>FITC</topic><topic>fluorescein isothiocyanate</topic><topic>follicle stimulating hormone</topic><topic>Follicle Stimulating Hormone - antagonists & inhibitors</topic><topic>FSH</topic><topic>Galectin 1 - antagonists & inhibitors</topic><topic>Galectin 1 - metabolism</topic><topic>Galectin 1 - pharmacology</topic><topic>galectin-1</topic><topic>granulosa cells</topic><topic>Granulosa Cells - drug effects</topic><topic>Granulosa Cells - metabolism</topic><topic>horseradish peroxidase</topic><topic>HRP</topic><topic>In Vitro Techniques</topic><topic>Lactose - analogs & derivatives</topic><topic>Lactose - pharmacology</topic><topic>mAb</topic><topic>monoclonal antibody</topic><topic>Ovary - cytology</topic><topic>Ovary - physiology</topic><topic>P450SCC</topic><topic>pAb</topic><topic>PAGE</topic><topic>PBS</topic><topic>PCR</topic><topic>phosphate buffered saline</topic><topic>pig ovaries</topic><topic>polyacrylamide gel electrophoresis</topic><topic>polyclonal antibody</topic><topic>polymerase chain reaction</topic><topic>Pregnenolone - pharmacology</topic><topic>Progesterone - antagonists & inhibitors</topic><topic>Progesterone - biosynthesis</topic><topic>Progesterone - metabolism</topic><topic>progesterone synthesis</topic><topic>reverse transcription</topic><topic>RNA, Messenger - antagonists & inhibitors</topic><topic>RNA, Messenger - genetics</topic><topic>SDS</topic><topic>sodium dodecyl sulfate</topic><topic>Swine</topic><topic>TBS</topic><topic>TCM</topic><topic>tissue culture medium</topic><topic>Tris-buffered saline</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walzel, Hermann</creatorcontrib><creatorcontrib>Brock, Josef</creatorcontrib><creatorcontrib>Pöhland, Ralf</creatorcontrib><creatorcontrib>Vanselow, Jens</creatorcontrib><creatorcontrib>Tomek, Wolfgang</creatorcontrib><creatorcontrib>Schneider, Falk</creatorcontrib><creatorcontrib>Tiemann, Ute</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Glycobiology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walzel, Hermann</au><au>Brock, Josef</au><au>Pöhland, Ralf</au><au>Vanselow, Jens</au><au>Tomek, Wolfgang</au><au>Schneider, Falk</au><au>Tiemann, Ute</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro</atitle><jtitle>Glycobiology (Oxford)</jtitle><addtitle>Glycobiology</addtitle><date>2004-10-01</date><risdate>2004</risdate><volume>14</volume><issue>10</issue><spage>871</spage><epage>881</epage><pages>871-881</pages><issn>0959-6658</issn><issn>1460-2423</issn><eissn>1460-2423</eissn><abstract>The detection of galectin-1 (gal-1) in pig granulosa cell lysates by immunoblotting and its cytosolic as well as membrane-associated localization prompted us to study its effects on cell proliferation and regulation of progesterone synthesis. The lectin stimulated the proliferation of granulosa cells from pig ovaries cultured in serum-free medium. Gal-1 inhibited the FSH-stimulated progesterone synthesis of granulosa cells. This inhibitory effect was strongly reduced by the disaccharidic competitor lactose at 30 mM. The absence of inhibitory effects on dibutyryl-cAMP (db-cAMP), forskolin, and pregnenolone-enhanced cellular progesterone synthesis suggests that gal-1interferes with the receptor-dependent mechanism of FSH-stimulated progesterone production. In FSH-stimulated granulosa cells, western blot analysis revealed the gal-1-mediated suppression of the cytochrome P450–dependent cholesterol side chain cleavage enzyme (P450SCC) that catalyzes the conversion of cholesterol to pregnenolone. In the presence of 30 mM lactose, the gal-1-reduced P450SCC expression was prevented. Strongly reduced mRNA levels were recorded for P450SCC and 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD) when FSH-stimulated granulosa cells were cultured in the presence of gal-1. We conclude that gal-1 exerts its inhibitory effect on steroidogenic activity of granulosa cells by interfering the hormone–receptor interaction resulting in decreased responses to FSH stimulation.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>15163626</pmid><doi>10.1093/glycob/cwh101</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-6658 |
| ispartof | Glycobiology (Oxford), 2004-10, Vol.14 (10), p.871-881 |
| issn | 0959-6658 1460-2423 1460-2423 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66783672 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | 3β-HSD 3β-hydroxysteroid dehydrogenase Animals bovine serum albumin BSA carbohydrate recognition domain Cattle Cell Proliferation - drug effects Colforsin - pharmacology CRD Cyclic AMP - pharmacology Cytochrome P-450 Enzyme Inhibitors Cytochrome P-450 Enzyme System - genetics cytochrome P450–dependent cholesterol side chain cleavage enzyme db-cAMP dibutyryl cyclic AMP ECL EDTA enhanced chemiluminescence ethylenediaminetetraacetic acid FCS Female fetal calf serum FITC fluorescein isothiocyanate follicle stimulating hormone Follicle Stimulating Hormone - antagonists & inhibitors FSH Galectin 1 - antagonists & inhibitors Galectin 1 - metabolism Galectin 1 - pharmacology galectin-1 granulosa cells Granulosa Cells - drug effects Granulosa Cells - metabolism horseradish peroxidase HRP In Vitro Techniques Lactose - analogs & derivatives Lactose - pharmacology mAb monoclonal antibody Ovary - cytology Ovary - physiology P450SCC pAb PAGE PBS PCR phosphate buffered saline pig ovaries polyacrylamide gel electrophoresis polyclonal antibody polymerase chain reaction Pregnenolone - pharmacology Progesterone - antagonists & inhibitors Progesterone - biosynthesis Progesterone - metabolism progesterone synthesis reverse transcription RNA, Messenger - antagonists & inhibitors RNA, Messenger - genetics SDS sodium dodecyl sulfate Swine TBS TCM tissue culture medium Tris-buffered saline |
| title | Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T06%3A17%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20galectin-1%20on%20regulation%20of%20progesterone%20production%20in%20granulosa%20cells%20from%20pig%20ovaries%20in%20vitro&rft.jtitle=Glycobiology%20(Oxford)&rft.au=Walzel,%20Hermann&rft.date=2004-10-01&rft.volume=14&rft.issue=10&rft.spage=871&rft.epage=881&rft.pages=871-881&rft.issn=0959-6658&rft.eissn=1460-2423&rft_id=info:doi/10.1093/glycob/cwh101&rft_dat=%3Cproquest_cross%3E729622281%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=210344002&rft_id=info:pmid/15163626&rfr_iscdi=true |