Microchip Laser-Induced Fluorescence Detection of Proteins at Submicrogram per Milliliter Levels Mediated by Dynamic Labeling under Pseudonative Conditions

We have previously demonstrated on-column dynamic labeling of protein−SDS complexes on capillaries and microchips for laser-induced fluorescence (LIF) detection using both a commercially available fluor and a protein separation buffer. Upon binding to hydrophobic moieties (of the analyte or separati...

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Veröffentlicht in:	Analytical chemistry (Washington) 2004-08, Vol.76 (16), p.4705-4714
Hauptverfasser:	Giordano, Braden C, Jin, Lianji, Couch, Abigail J, Ferrance, Jerome P, Landers, James P
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Blood Proteins - isolation & purification Chemistry Chromatographic methods and physical methods associated with chromatography Electrophoresis, Capillary - methods Electrophoresis, Polyacrylamide Gel Exact sciences and technology Humans Indicators and Reagents Lasers Microchip Analytical Procedures Other chromatographic methods Proteins - analysis Sensitivity and Specificity Sodium Dodecyl Sulfate Spectrometric and optical methods Spectrometry, Fluorescence Ultraviolet Rays
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