A Proteome Strategy for Fractionating Proteins and Peptides Using Continuous Free-Flow Electrophoresis Coupled Off-Line to Reversed-Phase High-Performance Liquid Chromatography

Extensive prefractionation is now considered to be a necessary prerequisite for the comprehensive analysis of complex proteomes where the dynamic range of protein abundances can vary from ∼106 for cells to ∼1010 for tissues such as blood. Here, we describe a high-resolution 2D protein separation sys...

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Veröffentlicht in:	Analytical chemistry (Washington) 2004-08, Vol.76 (16), p.4811-4824
Hauptverfasser:	Moritz, Robert L, Ji, Hong, Schütz, Frédéric, Connolly, Lisa M, Kapp, Eugene A, Speed, Terence P, Simpson, Richard J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Blood Cells Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Electrophoresis - instrumentation Electrophoresis - methods Equipment Design Exact sciences and technology Fundamental and applied biological sciences. Psychology Indicators and Reagents Liquids Other chromatographic methods Peptide Fragments - isolation & purification Peptides Peptides - isolation & purification Proteins Proteins - isolation & purification Proteome - isolation & purification Spectrometric and optical methods Tissues Trypsin - isolation & purification
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