Effects of puerarin on number and activity of endothelial progenitor cells from peripheral blood
To investigate whether puerarin can augment endothelial progenitor cells (EPCs) numbers, promote EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity. EPCs were characterized as adherent cells by double staining of DiLDL-uptake and lectin binding under a laser scanning confoc...
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| Veröffentlicht in: | Acta pharmacologica Sinica 2004-08, Vol.25 (8), p.1045-1051 |
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| container_title | Acta pharmacologica Sinica |
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| creator | Zhu, Jun-Hui Wang, Xing-Xiang Chen, Jun-Zhu Shang, Yun-Peng Zhu, Jian-Hua Guo, Xiao-Gang Sun, Jian |
| description | To investigate whether puerarin can augment endothelial progenitor cells (EPCs) numbers, promote EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity.
EPCs were characterized as adherent cells by double staining of DiLDL-uptake and lectin binding under a laser scanning confocal microscope. Expression of KDR, VEGFR-2, and AC133 was detected by flow cytometry. EPCs proliferation, migration and in vitro vasculogenesis were determined with MTT assay, modified Boyden chamber assay, and in vitro vasculogenesis kit, respectively. EPCs adhesive assay was performed by replating those on fibronectin-coated dishes, then adherent cells were counted.
Incubation of isolated human MNCs with puerarin 0.1-3 mmol/L increased the number of EPCs, EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity in a concentration- and time-dependent manner, which reached maximum at 3 mmol/L, 24 h (approximately 1-fold increase, P |
| format | Article |
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EPCs were characterized as adherent cells by double staining of DiLDL-uptake and lectin binding under a laser scanning confocal microscope. Expression of KDR, VEGFR-2, and AC133 was detected by flow cytometry. EPCs proliferation, migration and in vitro vasculogenesis were determined with MTT assay, modified Boyden chamber assay, and in vitro vasculogenesis kit, respectively. EPCs adhesive assay was performed by replating those on fibronectin-coated dishes, then adherent cells were counted.
Incubation of isolated human MNCs with puerarin 0.1-3 mmol/L increased the number of EPCs, EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity in a concentration- and time-dependent manner, which reached maximum at 3 mmol/L, 24 h (approximately 1-fold increase, P<0.01).
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EPCs were characterized as adherent cells by double staining of DiLDL-uptake and lectin binding under a laser scanning confocal microscope. Expression of KDR, VEGFR-2, and AC133 was detected by flow cytometry. EPCs proliferation, migration and in vitro vasculogenesis were determined with MTT assay, modified Boyden chamber assay, and in vitro vasculogenesis kit, respectively. EPCs adhesive assay was performed by replating those on fibronectin-coated dishes, then adherent cells were counted.
Incubation of isolated human MNCs with puerarin 0.1-3 mmol/L increased the number of EPCs, EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity in a concentration- and time-dependent manner, which reached maximum at 3 mmol/L, 24 h (approximately 1-fold increase, P<0.01).
Puerarin enhanced EPCs functional activity.</description><subject>AC133 Antigen</subject><subject>Antigens, CD</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Count</subject><subject>Cell Division - drug effects</subject><subject>Cell Movement - drug effects</subject><subject>Cells, Cultured</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - physiology</subject><subject>Glycoproteins - metabolism</subject><subject>Humans</subject><subject>Isoflavones - pharmacology</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Peptides - metabolism</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - physiology</subject><subject>Vascular Endothelial Growth Factor Receptor-2 - metabolism</subject><subject>Vasodilator Agents - pharmacology</subject><issn>1671-4083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEtLxDAcxHNQ3HX1K0hO3gp5tWmPsqwPWPCi55rHv24kTWqSCvvt7eJ6mYHhxzDMBVrTRtJKkJav0HXOX4Rwxml3hVa05oRK3q7Rx24YwJSM44CnGZJKLuAYcJhHDQmrYLEyxf24cjwhEGwsB_BOeTyl-AnBlZiwAe8zHlIc8QTJTYelyGPtY7Q36HJQPsPt2Tfo_XH3tn2u9q9PL9uHfTUxIktV87rjDTFCdpqSjmvBLIeOUSuk1FwoKVRjBdRMGdsa1ipLmKKmXnIjreYbdP_Xu8z6niGXfnT5tEsFiHPum0ZKucgC3p3BWY9g-ym5UaVj__8J_wWM5V2o</recordid><startdate>200408</startdate><enddate>200408</enddate><creator>Zhu, Jun-Hui</creator><creator>Wang, Xing-Xiang</creator><creator>Chen, Jun-Zhu</creator><creator>Shang, Yun-Peng</creator><creator>Zhu, Jian-Hua</creator><creator>Guo, Xiao-Gang</creator><creator>Sun, Jian</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200408</creationdate><title>Effects of puerarin on number and activity of endothelial progenitor cells from peripheral blood</title><author>Zhu, Jun-Hui ; Wang, Xing-Xiang ; Chen, Jun-Zhu ; Shang, Yun-Peng ; Zhu, Jian-Hua ; Guo, Xiao-Gang ; Sun, Jian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p207t-5359360c479b1093b42d3e921d477b34a74a6d4e52acd8c28ad02a1c574ac7db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>AC133 Antigen</topic><topic>Antigens, CD</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Count</topic><topic>Cell Division - drug effects</topic><topic>Cell Movement - drug effects</topic><topic>Cells, Cultured</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - physiology</topic><topic>Glycoproteins - metabolism</topic><topic>Humans</topic><topic>Isoflavones - pharmacology</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Peptides - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - physiology</topic><topic>Vascular Endothelial Growth Factor Receptor-2 - metabolism</topic><topic>Vasodilator Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Jun-Hui</creatorcontrib><creatorcontrib>Wang, Xing-Xiang</creatorcontrib><creatorcontrib>Chen, Jun-Zhu</creatorcontrib><creatorcontrib>Shang, Yun-Peng</creatorcontrib><creatorcontrib>Zhu, Jian-Hua</creatorcontrib><creatorcontrib>Guo, Xiao-Gang</creatorcontrib><creatorcontrib>Sun, Jian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Acta pharmacologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Jun-Hui</au><au>Wang, Xing-Xiang</au><au>Chen, Jun-Zhu</au><au>Shang, Yun-Peng</au><au>Zhu, Jian-Hua</au><au>Guo, Xiao-Gang</au><au>Sun, Jian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of puerarin on number and activity of endothelial progenitor cells from peripheral blood</atitle><jtitle>Acta pharmacologica Sinica</jtitle><addtitle>Acta Pharmacol Sin</addtitle><date>2004-08</date><risdate>2004</risdate><volume>25</volume><issue>8</issue><spage>1045</spage><epage>1051</epage><pages>1045-1051</pages><issn>1671-4083</issn><abstract>To investigate whether puerarin can augment endothelial progenitor cells (EPCs) numbers, promote EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity.
EPCs were characterized as adherent cells by double staining of DiLDL-uptake and lectin binding under a laser scanning confocal microscope. Expression of KDR, VEGFR-2, and AC133 was detected by flow cytometry. EPCs proliferation, migration and in vitro vasculogenesis were determined with MTT assay, modified Boyden chamber assay, and in vitro vasculogenesis kit, respectively. EPCs adhesive assay was performed by replating those on fibronectin-coated dishes, then adherent cells were counted.
Incubation of isolated human MNCs with puerarin 0.1-3 mmol/L increased the number of EPCs, EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity in a concentration- and time-dependent manner, which reached maximum at 3 mmol/L, 24 h (approximately 1-fold increase, P<0.01).
Puerarin enhanced EPCs functional activity.</abstract><cop>United States</cop><pmid>15301738</pmid><tpages>7</tpages></addata></record> |
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| ispartof | Acta pharmacologica Sinica, 2004-08, Vol.25 (8), p.1045-1051 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | AC133 Antigen Antigens, CD Cell Adhesion - drug effects Cell Count Cell Division - drug effects Cell Movement - drug effects Cells, Cultured Endothelium, Vascular - cytology Endothelium, Vascular - physiology Glycoproteins - metabolism Humans Isoflavones - pharmacology Neovascularization, Physiologic - drug effects Peptides - metabolism Stem Cells - cytology Stem Cells - physiology Vascular Endothelial Growth Factor Receptor-2 - metabolism Vasodilator Agents - pharmacology |
| title | Effects of puerarin on number and activity of endothelial progenitor cells from peripheral blood |
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