Detection of protein–DNA interactions in crude cellular extracts by fluorescence correlation spectroscopy

Fluorescence correlation spectroscopy (FCS) is a methodology to examine directly the translational diffusion of individual fluorescence-labeled molecules in solutions. Recent studies using FCS have quantified various bimolecular reactions without any need for amplification. To evaluate further the a...

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Veröffentlicht in:	Analytical biochemistry 2004-09, Vol.332 (1), p.58-66
Hauptverfasser:	Kobayashi, Tamiyo, Okamoto, Naoaki, Sawasaki, Tatsuya, Endo, Yaeta
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA - metabolism Fluorescence correlation spectroscopy HeLa Cells Humans JNK Mitogen-Activated Protein Kinases - metabolism Luminescent Measurements - methods NF-kappa B - metabolism Protein Binding - physiology Proteins - metabolism Protein–DNA interaction Spectrometry, Fluorescence - methods Transcription factor Transcription Factor AP-1 - metabolism Tumor Necrosis Factor-alpha - metabolism
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