High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids

We describe an E. coli‐based cell‐free system for the production of proteins with a non‐natural amino acid (nnAA) incorporated site‐specifically (modified protein). The mutant Methanococcus jannaschii tyrosyl‐tRNA synthetase (mTyrRS) and tRNATyr pair were used as orthogonal elements. The mTyrRS expe...

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Veröffentlicht in:	Biotechnology and bioengineering 2009-02, Vol.102 (2), p.400-416
Hauptverfasser:	Goerke, Aaron R., Swartz, James R.
Format:	Artikel
Sprache:	eng
Schlagworte:	(3 + 2) cycloaddition reaction Amino acids Amino Acids - metabolism Azides - metabolism Biochemistry Biological and medical sciences Biotechnology cell-free protein synthesis Cell-Free System - metabolism Cells Chemical synthesis click chemistry E coli E. coli inner membrane vesicles Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Methanococcus Methanococcus - enzymology Methanococcus jannaschii Mutation non-natural amino acid Phenylalanine - analogs & derivatives Phenylalanine - metabolism Protein Biosynthesis Proteins RNA, Transfer, Tyr - metabolism Tyrosine-tRNA Ligase - genetics Tyrosine-tRNA Ligase - metabolism unnatural amino acid
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