Na/K-ATPase assay in the intact guinea pig liver submitted to in situ perfusion

We describe an assay for the enzyme Na/K-ATPase in intact guinea pig livers perfused through the portal vein with modified Hank’s solution. The model uses the measurement of non-radioactive rubidium ion incorporation by liver cells, both in the absence and in the presence of the specific Na/K-ATPase...

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Veröffentlicht in:Analytical biochemistry 2009-02, Vol.385 (1), p.65-68
Hauptverfasser: dos Santos, Maria da Conceição B., Burth, Patricia, Younes-Ibrahim, Maurício, Gonçalves, Cassiano F., Santelli, Ricardo Erthal, Oliveira, Eliane Pádua, de Castro Faria, Mauro Velho
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Sprache:eng
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