Expression of leptin in two‐layered culture of gastric mucous cells and fibroblasts: effect of Helicobacter pylori attachment
Summary Background : Our recent histochemical studies have revealed an increase in myofibroblasts and in leptin and its receptor in endothelial cells, and myofibroblasts in Helicobacter pylori‐infected human and Mongolian gerbil fundic mucosa. Aim : The present study was undertaken to clarify the H....
Gespeichert in:
| Veröffentlicht in: | Alimentary pharmacology & therapeutics 2004-07, Vol.20 (s1), p.125-130 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 130 |
|---|---|
| container_issue | s1 |
| container_start_page | 125 |
| container_title | Alimentary pharmacology & therapeutics |
| container_volume | 20 |
| creator | Nakamura, M. Takahashi, T. Matsumoto, T. Akiba, Y. Matsui, H. Tsuchimoto, K. Ishii, H. Yamada, H. |
| description | Summary
Background : Our recent histochemical studies have revealed an increase in myofibroblasts and in leptin and its receptor in endothelial cells, and myofibroblasts in Helicobacter pylori‐infected human and Mongolian gerbil fundic mucosa.
Aim : The present study was undertaken to clarify the H. pylori–induced interaction between leptin in cultured gastric surface mucous cells and fibroblasts.
Methods : GSM06 cells were incubated with an air− liquid interface on a collagen gel layer containing mouse fibroblast cell line L929. Medium containing H. pylori bacilli (ATCC43504) at 10–100 times higher concentration than the GSM06 cells was added from the luminal side and the localization of leptin was observed by immunohistochemistry. The transformation of L929 cells to myofibroblasts was detected by electron microscopy and PR 2D3 immunoreactivity.
Results : L929 cells in the control group showed a spindle shape with scarce cytoplasm. In the H. pylori‐treated group, L929 cells showed features characteristic of myofibroblasts, and most GSM06 and L929 cells showed leptin immunoreactivity. In contrast, L929 cells incubated with H. pylori alone did not undergo this differentiation.
Conclusions : Attachment of H. pylori to surface epithelial cells caused conversion of fibroblasts to myofibroblasts. We suggest that leptin plays a role in this transformation. |
| doi_str_mv | 10.1111/j.1365-2036.2004.01981.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66772811</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66772811</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4151-28135bb64a1a03fcf0d8644a3d02da19045d4f233208342df28a456605866e5d3</originalsourceid><addsrcrecordid>eNqNkM1u1DAQgC0EokvhFZBP3Db1X7xeJA5VVShSJTiUs-XYY_DKiYPtqLun8gg8I0_SpLuCK5YljzTfzHg-hDAlDZ3Pxa6hXLZrRrhsGCGiIXSraLN_hlZ_E8_RijC5XTNF-Rl6VcqOECI3hL1EZ7RlWyWpWqGH6_2YoZSQBpw8jjDWMOD51vv059fvaA6QwWE7xTplWJDvptQcLO4nm6aCLcRYsBkc9qHLqYtzurzH4D3YuvA3EINNnbEVMh4PMeWATa3G_uhhqK_RC29igTen9xx9-3h9d3Wzvv3y6fPV5e3aCtrSpyXarpPCUEO4t544JYUw3BHmDN0S0TrhGeeMKC6Y80wZ0UpJWiUltI6fo3fHvmNOPycoVfehLH83A8xraCk3m3kInUF1BG1OpWTwesyhN_mgKdGLfL3Ti2O9ONaLfP0kX-_n0renGVPXg_tXeLI9Ax-OwH2IcPjvxvry690S8UdgKZVB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66772811</pqid></control><display><type>article</type><title>Expression of leptin in two‐layered culture of gastric mucous cells and fibroblasts: effect of Helicobacter pylori attachment</title><source>IngentaConnect Backfiles</source><source>MEDLINE</source><source>Wiley Online Library Journals</source><source>Wiley Online Library Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Nakamura, M. ; Takahashi, T. ; Matsumoto, T. ; Akiba, Y. ; Matsui, H. ; Tsuchimoto, K. ; Ishii, H. ; Yamada, H.</creator><creatorcontrib>Nakamura, M. ; Takahashi, T. ; Matsumoto, T. ; Akiba, Y. ; Matsui, H. ; Tsuchimoto, K. ; Ishii, H. ; Yamada, H.</creatorcontrib><description>Summary
Background : Our recent histochemical studies have revealed an increase in myofibroblasts and in leptin and its receptor in endothelial cells, and myofibroblasts in Helicobacter pylori‐infected human and Mongolian gerbil fundic mucosa.
Aim : The present study was undertaken to clarify the H. pylori–induced interaction between leptin in cultured gastric surface mucous cells and fibroblasts.
Methods : GSM06 cells were incubated with an air− liquid interface on a collagen gel layer containing mouse fibroblast cell line L929. Medium containing H. pylori bacilli (ATCC43504) at 10–100 times higher concentration than the GSM06 cells was added from the luminal side and the localization of leptin was observed by immunohistochemistry. The transformation of L929 cells to myofibroblasts was detected by electron microscopy and PR 2D3 immunoreactivity.
Results : L929 cells in the control group showed a spindle shape with scarce cytoplasm. In the H. pylori‐treated group, L929 cells showed features characteristic of myofibroblasts, and most GSM06 and L929 cells showed leptin immunoreactivity. In contrast, L929 cells incubated with H. pylori alone did not undergo this differentiation.
Conclusions : Attachment of H. pylori to surface epithelial cells caused conversion of fibroblasts to myofibroblasts. We suggest that leptin plays a role in this transformation.</description><identifier>ISSN: 0269-2813</identifier><identifier>EISSN: 1365-2036</identifier><identifier>DOI: 10.1111/j.1365-2036.2004.01981.x</identifier><identifier>PMID: 15298618</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Bacterial Adhesion - physiology ; Cell Differentiation ; Cells, Cultured ; Fibroblasts - metabolism ; Fibroblasts - microbiology ; Fibroblasts - ultrastructure ; Gastric Mucosa - metabolism ; Gastric Mucosa - microbiology ; Gastric Mucosa - ultrastructure ; Helicobacter pylori - physiology ; Leptin - metabolism ; Mice ; Mice, Transgenic ; Microscopy, Electron</subject><ispartof>Alimentary pharmacology & therapeutics, 2004-07, Vol.20 (s1), p.125-130</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4151-28135bb64a1a03fcf0d8644a3d02da19045d4f233208342df28a456605866e5d3</citedby><cites>FETCH-LOGICAL-c4151-28135bb64a1a03fcf0d8644a3d02da19045d4f233208342df28a456605866e5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2036.2004.01981.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2036.2004.01981.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15298618$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakamura, M.</creatorcontrib><creatorcontrib>Takahashi, T.</creatorcontrib><creatorcontrib>Matsumoto, T.</creatorcontrib><creatorcontrib>Akiba, Y.</creatorcontrib><creatorcontrib>Matsui, H.</creatorcontrib><creatorcontrib>Tsuchimoto, K.</creatorcontrib><creatorcontrib>Ishii, H.</creatorcontrib><creatorcontrib>Yamada, H.</creatorcontrib><title>Expression of leptin in two‐layered culture of gastric mucous cells and fibroblasts: effect of Helicobacter pylori attachment</title><title>Alimentary pharmacology & therapeutics</title><addtitle>Aliment Pharmacol Ther</addtitle><description>Summary
Background : Our recent histochemical studies have revealed an increase in myofibroblasts and in leptin and its receptor in endothelial cells, and myofibroblasts in Helicobacter pylori‐infected human and Mongolian gerbil fundic mucosa.
Aim : The present study was undertaken to clarify the H. pylori–induced interaction between leptin in cultured gastric surface mucous cells and fibroblasts.
Methods : GSM06 cells were incubated with an air− liquid interface on a collagen gel layer containing mouse fibroblast cell line L929. Medium containing H. pylori bacilli (ATCC43504) at 10–100 times higher concentration than the GSM06 cells was added from the luminal side and the localization of leptin was observed by immunohistochemistry. The transformation of L929 cells to myofibroblasts was detected by electron microscopy and PR 2D3 immunoreactivity.
Results : L929 cells in the control group showed a spindle shape with scarce cytoplasm. In the H. pylori‐treated group, L929 cells showed features characteristic of myofibroblasts, and most GSM06 and L929 cells showed leptin immunoreactivity. In contrast, L929 cells incubated with H. pylori alone did not undergo this differentiation.
Conclusions : Attachment of H. pylori to surface epithelial cells caused conversion of fibroblasts to myofibroblasts. We suggest that leptin plays a role in this transformation.</description><subject>Animals</subject><subject>Bacterial Adhesion - physiology</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - microbiology</subject><subject>Fibroblasts - ultrastructure</subject><subject>Gastric Mucosa - metabolism</subject><subject>Gastric Mucosa - microbiology</subject><subject>Gastric Mucosa - ultrastructure</subject><subject>Helicobacter pylori - physiology</subject><subject>Leptin - metabolism</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Microscopy, Electron</subject><issn>0269-2813</issn><issn>1365-2036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1u1DAQgC0EokvhFZBP3Db1X7xeJA5VVShSJTiUs-XYY_DKiYPtqLun8gg8I0_SpLuCK5YljzTfzHg-hDAlDZ3Pxa6hXLZrRrhsGCGiIXSraLN_hlZ_E8_RijC5XTNF-Rl6VcqOECI3hL1EZ7RlWyWpWqGH6_2YoZSQBpw8jjDWMOD51vv059fvaA6QwWE7xTplWJDvptQcLO4nm6aCLcRYsBkc9qHLqYtzurzH4D3YuvA3EINNnbEVMh4PMeWATa3G_uhhqK_RC29igTen9xx9-3h9d3Wzvv3y6fPV5e3aCtrSpyXarpPCUEO4t544JYUw3BHmDN0S0TrhGeeMKC6Y80wZ0UpJWiUltI6fo3fHvmNOPycoVfehLH83A8xraCk3m3kInUF1BG1OpWTwesyhN_mgKdGLfL3Ti2O9ONaLfP0kX-_n0renGVPXg_tXeLI9Ax-OwH2IcPjvxvry690S8UdgKZVB</recordid><startdate>200407</startdate><enddate>200407</enddate><creator>Nakamura, M.</creator><creator>Takahashi, T.</creator><creator>Matsumoto, T.</creator><creator>Akiba, Y.</creator><creator>Matsui, H.</creator><creator>Tsuchimoto, K.</creator><creator>Ishii, H.</creator><creator>Yamada, H.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200407</creationdate><title>Expression of leptin in two‐layered culture of gastric mucous cells and fibroblasts: effect of Helicobacter pylori attachment</title><author>Nakamura, M. ; Takahashi, T. ; Matsumoto, T. ; Akiba, Y. ; Matsui, H. ; Tsuchimoto, K. ; Ishii, H. ; Yamada, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4151-28135bb64a1a03fcf0d8644a3d02da19045d4f233208342df28a456605866e5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Bacterial Adhesion - physiology</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - microbiology</topic><topic>Fibroblasts - ultrastructure</topic><topic>Gastric Mucosa - metabolism</topic><topic>Gastric Mucosa - microbiology</topic><topic>Gastric Mucosa - ultrastructure</topic><topic>Helicobacter pylori - physiology</topic><topic>Leptin - metabolism</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Microscopy, Electron</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakamura, M.</creatorcontrib><creatorcontrib>Takahashi, T.</creatorcontrib><creatorcontrib>Matsumoto, T.</creatorcontrib><creatorcontrib>Akiba, Y.</creatorcontrib><creatorcontrib>Matsui, H.</creatorcontrib><creatorcontrib>Tsuchimoto, K.</creatorcontrib><creatorcontrib>Ishii, H.</creatorcontrib><creatorcontrib>Yamada, H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Alimentary pharmacology & therapeutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakamura, M.</au><au>Takahashi, T.</au><au>Matsumoto, T.</au><au>Akiba, Y.</au><au>Matsui, H.</au><au>Tsuchimoto, K.</au><au>Ishii, H.</au><au>Yamada, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of leptin in two‐layered culture of gastric mucous cells and fibroblasts: effect of Helicobacter pylori attachment</atitle><jtitle>Alimentary pharmacology & therapeutics</jtitle><addtitle>Aliment Pharmacol Ther</addtitle><date>2004-07</date><risdate>2004</risdate><volume>20</volume><issue>s1</issue><spage>125</spage><epage>130</epage><pages>125-130</pages><issn>0269-2813</issn><eissn>1365-2036</eissn><abstract>Summary
Background : Our recent histochemical studies have revealed an increase in myofibroblasts and in leptin and its receptor in endothelial cells, and myofibroblasts in Helicobacter pylori‐infected human and Mongolian gerbil fundic mucosa.
Aim : The present study was undertaken to clarify the H. pylori–induced interaction between leptin in cultured gastric surface mucous cells and fibroblasts.
Methods : GSM06 cells were incubated with an air− liquid interface on a collagen gel layer containing mouse fibroblast cell line L929. Medium containing H. pylori bacilli (ATCC43504) at 10–100 times higher concentration than the GSM06 cells was added from the luminal side and the localization of leptin was observed by immunohistochemistry. The transformation of L929 cells to myofibroblasts was detected by electron microscopy and PR 2D3 immunoreactivity.
Results : L929 cells in the control group showed a spindle shape with scarce cytoplasm. In the H. pylori‐treated group, L929 cells showed features characteristic of myofibroblasts, and most GSM06 and L929 cells showed leptin immunoreactivity. In contrast, L929 cells incubated with H. pylori alone did not undergo this differentiation.
Conclusions : Attachment of H. pylori to surface epithelial cells caused conversion of fibroblasts to myofibroblasts. We suggest that leptin plays a role in this transformation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>15298618</pmid><doi>10.1111/j.1365-2036.2004.01981.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0269-2813 |
| ispartof | Alimentary pharmacology & therapeutics, 2004-07, Vol.20 (s1), p.125-130 |
| issn | 0269-2813 1365-2036 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66772811 |
| source | IngentaConnect Backfiles; MEDLINE; Wiley Online Library Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Bacterial Adhesion - physiology Cell Differentiation Cells, Cultured Fibroblasts - metabolism Fibroblasts - microbiology Fibroblasts - ultrastructure Gastric Mucosa - metabolism Gastric Mucosa - microbiology Gastric Mucosa - ultrastructure Helicobacter pylori - physiology Leptin - metabolism Mice Mice, Transgenic Microscopy, Electron |
| title | Expression of leptin in two‐layered culture of gastric mucous cells and fibroblasts: effect of Helicobacter pylori attachment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T09%3A44%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20leptin%20in%20two%E2%80%90layered%20culture%20of%20gastric%20mucous%20cells%20and%20fibroblasts:%20effect%20of%20Helicobacter%20pylori%20attachment&rft.jtitle=Alimentary%20pharmacology%20&%20therapeutics&rft.au=Nakamura,%20M.&rft.date=2004-07&rft.volume=20&rft.issue=s1&rft.spage=125&rft.epage=130&rft.pages=125-130&rft.issn=0269-2813&rft.eissn=1365-2036&rft_id=info:doi/10.1111/j.1365-2036.2004.01981.x&rft_dat=%3Cproquest_cross%3E66772811%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=66772811&rft_id=info:pmid/15298618&rfr_iscdi=true |