Development of polymerase chain reaction and comparison with in situ hybridization for the detection of Haemophilus parasuis in formalin-fixed, paraffin-embedded tissues

DNA extraction and nested polymerase chain reaction (PCR) were developed for the detection of Haemophilus parasuis from formalin-fixed, paraffin-embedded tissues. The results for nested PCR were compared with those determined by in situ hybridization. The optimal results obtained show that use of xy...

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Veröffentlicht in:	Journal of Veterinary Medical Science 2004, Vol.66(7), pp.841-845
Hauptverfasser:	Jung, K. (Seoul National Univ. (Korea R.)), Ha, Y, Kim, S.H, Chae, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinobacillus pleuropneumoniae - genetics Animals Colostrum DNA DNA extraction DNA Primers - chemistry DNA, Bacterial - analysis Formaldehyde HAEMOPHILUS Haemophilus Infections - diagnosis Haemophilus Infections - microbiology Haemophilus Infections - veterinary Haemophilus parasuis Haemophilus parasuis - genetics Haemophilus parasuis - isolation & purification HISTOPATHOLOGY HYBRIDIZATION in situ hybridization In Situ Hybridization - methods In Situ Hybridization - veterinary Liver - microbiology METHODS Paraffin Embedding - veterinary Pasteurella multocida - genetics PCR Pericardium - microbiology Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Sensitivity and Specificity Sequence Analysis, DNA Spleen - microbiology SWINE Swine - microbiology Swine Diseases - diagnosis Swine Diseases - microbiology Tissue Fixation - veterinary
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