Development of high performance liquid chromatography method for lacidipine in rabbit serum: Application to pharmacokinetic study
A simple and sensitive high performance liquid chromatographic (HPLC) method for quantification of lacidipine (LCDP) in rabbit serum was developed and validated. LCDP and internal standard (IS), felodipine were extracted into n-hexane and dichloromethane (70:30) solvent system and separated using an...
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Veröffentlicht in: | Analytica chimica acta 2009-01, Vol.632 (2), p.278-283 |
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container_title | Analytica chimica acta |
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creator | Ramesh, Gannu Vamshi Vishnu, Yamsani Chinna Reddy, Palem Shravan Kumar, Yamsani Madhusudan Rao, Yamsani |
description | A simple and sensitive high performance liquid chromatographic (HPLC) method for quantification of lacidipine (LCDP) in rabbit serum was developed and validated. LCDP and internal standard (IS), felodipine were extracted into n-hexane and dichloromethane (70:30) solvent system and separated using an isocratic mobile phase, on an Inertsil C18 column. The effluent was monitored by UV detector at 240
nm and at a flow rate of 1.0
mL
min
−1. The linearity range of proposed method was 1–500
ng
mL
−1. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 94 and 95% for LCDP and IS, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of lacidipine after application of LCDP microemulsion gel in rabbits. |
doi_str_mv | 10.1016/j.aca.2008.11.018 |
format | Article |
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nm and at a flow rate of 1.0
mL
min
−1. The linearity range of proposed method was 1–500
ng
mL
−1. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 94 and 95% for LCDP and IS, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of lacidipine after application of LCDP microemulsion gel in rabbits.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2008.11.018</identifier><identifier>PMID: 19110105</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Animals ; Biological and medical sciences ; Calcium Channel Blockers - blood ; Calcium Channel Blockers - isolation & purification ; Calcium Channel Blockers - pharmacokinetics ; Calibration ; Chemical Precipitation ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, High Pressure Liquid - economics ; Chromatography, High Pressure Liquid - methods ; Costs and Cost Analysis ; Dihydropyridines - blood ; Dihydropyridines - isolation & purification ; Dihydropyridines - pharmacokinetics ; Exact sciences and technology ; General pharmacology ; Lacidipine ; Medical sciences ; Other chromatographic methods ; Pharmacokinetics ; Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions ; Pharmacology. Drug treatments ; Rabbit serum ; Rabbits ; Reversed phase high performance liquid chromatography ; Sensitivity and Specificity ; Temperature ; Time Factors</subject><ispartof>Analytica chimica acta, 2009-01, Vol.632 (2), p.278-283</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-4e6507c53282e662fc9a2698f52295bfc4d28f1554de363785c641c058501ea83</citedby><cites>FETCH-LOGICAL-c412t-4e6507c53282e662fc9a2698f52295bfc4d28f1554de363785c641c058501ea83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003267008019107$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21073271$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19110105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramesh, Gannu</creatorcontrib><creatorcontrib>Vamshi Vishnu, Yamsani</creatorcontrib><creatorcontrib>Chinna Reddy, Palem</creatorcontrib><creatorcontrib>Shravan Kumar, Yamsani</creatorcontrib><creatorcontrib>Madhusudan Rao, Yamsani</creatorcontrib><title>Development of high performance liquid chromatography method for lacidipine in rabbit serum: Application to pharmacokinetic study</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>A simple and sensitive high performance liquid chromatographic (HPLC) method for quantification of lacidipine (LCDP) in rabbit serum was developed and validated. LCDP and internal standard (IS), felodipine were extracted into n-hexane and dichloromethane (70:30) solvent system and separated using an isocratic mobile phase, on an Inertsil C18 column. The effluent was monitored by UV detector at 240
nm and at a flow rate of 1.0
mL
min
−1. The linearity range of proposed method was 1–500
ng
mL
−1. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 94 and 95% for LCDP and IS, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of lacidipine after application of LCDP microemulsion gel in rabbits.</description><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium Channel Blockers - blood</subject><subject>Calcium Channel Blockers - isolation & purification</subject><subject>Calcium Channel Blockers - pharmacokinetics</subject><subject>Calibration</subject><subject>Chemical Precipitation</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, High Pressure Liquid - economics</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Costs and Cost Analysis</subject><subject>Dihydropyridines - blood</subject><subject>Dihydropyridines - isolation & purification</subject><subject>Dihydropyridines - pharmacokinetics</subject><subject>Exact sciences and technology</subject><subject>General pharmacology</subject><subject>Lacidipine</subject><subject>Medical sciences</subject><subject>Other chromatographic methods</subject><subject>Pharmacokinetics</subject><subject>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbit serum</subject><subject>Rabbits</subject><subject>Reversed phase high performance liquid chromatography</subject><subject>Sensitivity and Specificity</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhB3BBvsAtwePEdgKnqnxKlbjA2fI6k8ZLEru2U2mP_HNc7QpucBqN9LyvRvMQ8hJYDQzk20NtrKk5Y10NUDPoHpEddKqp2oa3j8mOMdZUXCp2QZ6ldCgrB9Y-JRfQQylgYkd-fcB7nH1YcM3Uj3RytxMNGEcfF7NapLO729xA7RT9YrK_jSZMR7pgnvxAC0VnY93ggluRupVGs9-7TBPGbXlHr0KYnTXZ-ZVmT8NkSqv1PwucnaUpb8PxOXkymjnhi_O8JD8-ffx-_aW6-fb56_XVTWVb4LlqUQqmrGh4x1FKPtrecNl3o-C8F_vRtgPvRhCiHbCRjeqElS1YJjrBAE3XXJI3p94Q_d2GKevFJYvzbFb0W9JSKtn36v9gI3rGRasKCCfQRp9SxFGH6BYTjxqYfhCkD7oI0g-CNIAugkrm1bl82y84_E2cjRTg9RkwyZp5jMWCS3-4IlA1XEHh3p84LD-7dxh1sg6LscFFtFkP3v3jjN-uFa7k</recordid><startdate>20090126</startdate><enddate>20090126</enddate><creator>Ramesh, Gannu</creator><creator>Vamshi Vishnu, Yamsani</creator><creator>Chinna Reddy, Palem</creator><creator>Shravan Kumar, Yamsani</creator><creator>Madhusudan Rao, Yamsani</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20090126</creationdate><title>Development of high performance liquid chromatography method for lacidipine in rabbit serum: Application to pharmacokinetic study</title><author>Ramesh, Gannu ; Vamshi Vishnu, Yamsani ; Chinna Reddy, Palem ; Shravan Kumar, Yamsani ; Madhusudan Rao, Yamsani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-4e6507c53282e662fc9a2698f52295bfc4d28f1554de363785c641c058501ea83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium Channel Blockers - blood</topic><topic>Calcium Channel Blockers - isolation & purification</topic><topic>Calcium Channel Blockers - pharmacokinetics</topic><topic>Calibration</topic><topic>Chemical Precipitation</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, High Pressure Liquid - economics</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Costs and Cost Analysis</topic><topic>Dihydropyridines - blood</topic><topic>Dihydropyridines - isolation & purification</topic><topic>Dihydropyridines - pharmacokinetics</topic><topic>Exact sciences and technology</topic><topic>General pharmacology</topic><topic>Lacidipine</topic><topic>Medical sciences</topic><topic>Other chromatographic methods</topic><topic>Pharmacokinetics</topic><topic>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbit serum</topic><topic>Rabbits</topic><topic>Reversed phase high performance liquid chromatography</topic><topic>Sensitivity and Specificity</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramesh, Gannu</creatorcontrib><creatorcontrib>Vamshi Vishnu, Yamsani</creatorcontrib><creatorcontrib>Chinna Reddy, Palem</creatorcontrib><creatorcontrib>Shravan Kumar, Yamsani</creatorcontrib><creatorcontrib>Madhusudan Rao, Yamsani</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramesh, Gannu</au><au>Vamshi Vishnu, Yamsani</au><au>Chinna Reddy, Palem</au><au>Shravan Kumar, Yamsani</au><au>Madhusudan Rao, Yamsani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of high performance liquid chromatography method for lacidipine in rabbit serum: Application to pharmacokinetic study</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2009-01-26</date><risdate>2009</risdate><volume>632</volume><issue>2</issue><spage>278</spage><epage>283</epage><pages>278-283</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>A simple and sensitive high performance liquid chromatographic (HPLC) method for quantification of lacidipine (LCDP) in rabbit serum was developed and validated. LCDP and internal standard (IS), felodipine were extracted into n-hexane and dichloromethane (70:30) solvent system and separated using an isocratic mobile phase, on an Inertsil C18 column. The effluent was monitored by UV detector at 240
nm and at a flow rate of 1.0
mL
min
−1. The linearity range of proposed method was 1–500
ng
mL
−1. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 94 and 95% for LCDP and IS, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of lacidipine after application of LCDP microemulsion gel in rabbits.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19110105</pmid><doi>10.1016/j.aca.2008.11.018</doi><tpages>6</tpages></addata></record> |
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subjects | Analytical chemistry Animals Biological and medical sciences Calcium Channel Blockers - blood Calcium Channel Blockers - isolation & purification Calcium Channel Blockers - pharmacokinetics Calibration Chemical Precipitation Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - economics Chromatography, High Pressure Liquid - methods Costs and Cost Analysis Dihydropyridines - blood Dihydropyridines - isolation & purification Dihydropyridines - pharmacokinetics Exact sciences and technology General pharmacology Lacidipine Medical sciences Other chromatographic methods Pharmacokinetics Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions Pharmacology. Drug treatments Rabbit serum Rabbits Reversed phase high performance liquid chromatography Sensitivity and Specificity Temperature Time Factors |
title | Development of high performance liquid chromatography method for lacidipine in rabbit serum: Application to pharmacokinetic study |
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