Development of high performance liquid chromatography method for lacidipine in rabbit serum: Application to pharmacokinetic study

A simple and sensitive high performance liquid chromatographic (HPLC) method for quantification of lacidipine (LCDP) in rabbit serum was developed and validated. LCDP and internal standard (IS), felodipine were extracted into n-hexane and dichloromethane (70:30) solvent system and separated using an...

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Veröffentlicht in:Analytica chimica acta 2009-01, Vol.632 (2), p.278-283
Hauptverfasser: Ramesh, Gannu, Vamshi Vishnu, Yamsani, Chinna Reddy, Palem, Shravan Kumar, Yamsani, Madhusudan Rao, Yamsani
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container_issue 2
container_start_page 278
container_title Analytica chimica acta
container_volume 632
creator Ramesh, Gannu
Vamshi Vishnu, Yamsani
Chinna Reddy, Palem
Shravan Kumar, Yamsani
Madhusudan Rao, Yamsani
description A simple and sensitive high performance liquid chromatographic (HPLC) method for quantification of lacidipine (LCDP) in rabbit serum was developed and validated. LCDP and internal standard (IS), felodipine were extracted into n-hexane and dichloromethane (70:30) solvent system and separated using an isocratic mobile phase, on an Inertsil C18 column. The effluent was monitored by UV detector at 240 nm and at a flow rate of 1.0 mL min −1. The linearity range of proposed method was 1–500 ng mL −1. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 94 and 95% for LCDP and IS, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of lacidipine after application of LCDP microemulsion gel in rabbits.
doi_str_mv 10.1016/j.aca.2008.11.018
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LCDP and internal standard (IS), felodipine were extracted into n-hexane and dichloromethane (70:30) solvent system and separated using an isocratic mobile phase, on an Inertsil C18 column. The effluent was monitored by UV detector at 240 nm and at a flow rate of 1.0 mL min −1. The linearity range of proposed method was 1–500 ng mL −1. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 94 and 95% for LCDP and IS, respectively and the method was found to be precise, accurate, and specific during the study. 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LCDP and internal standard (IS), felodipine were extracted into n-hexane and dichloromethane (70:30) solvent system and separated using an isocratic mobile phase, on an Inertsil C18 column. The effluent was monitored by UV detector at 240 nm and at a flow rate of 1.0 mL min −1. The linearity range of proposed method was 1–500 ng mL −1. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 94 and 95% for LCDP and IS, respectively and the method was found to be precise, accurate, and specific during the study. 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Pharmacogenetics. Drug-receptor interactions</subject><subject>Pharmacology. 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LCDP and internal standard (IS), felodipine were extracted into n-hexane and dichloromethane (70:30) solvent system and separated using an isocratic mobile phase, on an Inertsil C18 column. The effluent was monitored by UV detector at 240 nm and at a flow rate of 1.0 mL min −1. The linearity range of proposed method was 1–500 ng mL −1. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 94 and 95% for LCDP and IS, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of lacidipine after application of LCDP microemulsion gel in rabbits.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19110105</pmid><doi>10.1016/j.aca.2008.11.018</doi><tpages>6</tpages></addata></record>
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subjects Analytical chemistry
Animals
Biological and medical sciences
Calcium Channel Blockers - blood
Calcium Channel Blockers - isolation & purification
Calcium Channel Blockers - pharmacokinetics
Calibration
Chemical Precipitation
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - economics
Chromatography, High Pressure Liquid - methods
Costs and Cost Analysis
Dihydropyridines - blood
Dihydropyridines - isolation & purification
Dihydropyridines - pharmacokinetics
Exact sciences and technology
General pharmacology
Lacidipine
Medical sciences
Other chromatographic methods
Pharmacokinetics
Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions
Pharmacology. Drug treatments
Rabbit serum
Rabbits
Reversed phase high performance liquid chromatography
Sensitivity and Specificity
Temperature
Time Factors
title Development of high performance liquid chromatography method for lacidipine in rabbit serum: Application to pharmacokinetic study
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