CYP450 2B4 covalently attached to carbon and gold screen printed electrodes by diazonium salt and thiols monolayers

An easy covalent immobilization method used to develop enzyme biosensors based on carbon and gold screen printed electrodes (SPCEs and gold SPEs) is described. The linkage of biomolecules through 4-nitrobenzenediazonium tetrafluoroborate, mercaptopropionic acid and thioctic acid monolayers has been...

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Veröffentlicht in:	Analytica chimica acta 2009-02, Vol.633 (1), p.51-56
Hauptverfasser:	Alonso-Lomillo, M.A., Yardimci, C., Domínguez-Renedo, O., Arcos-Martínez, M.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aryl Hydrocarbon Hydroxylases - chemical synthesis Aryl Hydrocarbon Hydroxylases - chemistry Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensor Biosensors Biotechnology Carbon - chemistry Cytochrome P450 Family 2 Diazonium Compounds - chemical synthesis Diazonium Compounds - chemistry Diazonium salt Electrodes Fundamental and applied biological sciences. Psychology Gold - chemistry Gold nanoparticles Methods. Procedures. Technologies Nanoparticles - chemistry Phenobarbital - analysis Reproducibility of Results Screen printed electrodes Self-assembled monolayer Sulfhydryl Compounds - chemical synthesis Sulfhydryl Compounds - chemistry Various methods and equipments
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