The accumulation of alpha-zein in transgenic tobacco endosperm is stabilized by co-expression of beta-zein
The cysteine-poor alpha-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with delta-zein, whereas the hydrophobic cysteine-rich beta- and gamma-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressin...
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| Veröffentlicht in: | Plant and cell physiology 2004-07, Vol.45 (7), p.864-871 |
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| container_title | Plant and cell physiology |
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| creator | Coleman, C.E. (Brigham Young Univ., Utah (USA)) Yoho, P.R Escobar, S Ogawa, M |
| description | The cysteine-poor alpha-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with delta-zein, whereas the hydrophobic cysteine-rich beta- and gamma-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressing zein genes, alpha-zein was unstable unless coexpressed with gamma-zein. Here we showed that alpha-zein was also stabilized by beta-zein. Small accretions of alpha- and beta-zeins, similar in appearance to maize protein bodies, were localized to the endoplasmic reticulum within tobacco endosperm cells. The zein proteins were also localized to protein storage vacuoles in a more dispersed pattern, suggesting that they were transported there after they were post-translationally sequestered into the ER. |
| doi_str_mv | 10.1093/pcp/pch104 |
| format | Article |
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The zein proteins were also localized to protein storage vacuoles in a more dispersed pattern, suggesting that they were transported there after they were post-translationally sequestered into the ER.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pch104</identifier><identifier>PMID: 15295069</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>dap ; days after pollination ; endoplasmic reticulum ; Endoplasmic Reticulum - genetics ; Endoplasmic Reticulum - metabolism ; Endoplasmic Reticulum - ultrastructure ; ENDOSPERM ; Gene Expression Regulation, Plant - genetics ; Keywords: Endosperm — Maize — Protein-body — Transgenic — Tobacco — Zein ; Microscopy, Electron ; Multigene Family ; Nicotiana - genetics ; Nicotiana - growth & development ; Nicotiana - metabolism ; NICOTIANA TABACUM ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - growth & development ; Plants, Genetically Modified - metabolism ; PROLAMINES ; Protein Isoforms - biosynthesis ; Protein Isoforms - genetics ; Protein Processing, Post-Translational - genetics ; protein storage vacuole ; PSV ; rER ; rough endoplasmic reticulum ; Seeds - genetics ; Seeds - metabolism ; Seeds - ultrastructure ; STORAGE PROTEINS ; Transfection ; TRANSGENIC PLANTS ; Vacuoles - genetics ; Vacuoles - metabolism ; Vacuoles - ultrastructure ; ZEA MAYS ; Zea mays - genetics ; Zea mays - metabolism ; Zein - biosynthesis ; Zein - genetics</subject><ispartof>Plant and cell physiology, 2004-07, Vol.45 (7), p.864-871</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-83ae61090e777f90859f5b153663f0e8cf7bb25dd75196af870de2d8db7fac0b3</citedby><cites>FETCH-LOGICAL-c410t-83ae61090e777f90859f5b153663f0e8cf7bb25dd75196af870de2d8db7fac0b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15295069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coleman, C.E. (Brigham Young Univ., Utah (USA))</creatorcontrib><creatorcontrib>Yoho, P.R</creatorcontrib><creatorcontrib>Escobar, S</creatorcontrib><creatorcontrib>Ogawa, M</creatorcontrib><title>The accumulation of alpha-zein in transgenic tobacco endosperm is stabilized by co-expression of beta-zein</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>The cysteine-poor alpha-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with delta-zein, whereas the hydrophobic cysteine-rich beta- and gamma-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressing zein genes, alpha-zein was unstable unless coexpressed with gamma-zein. Here we showed that alpha-zein was also stabilized by beta-zein. Small accretions of alpha- and beta-zeins, similar in appearance to maize protein bodies, were localized to the endoplasmic reticulum within tobacco endosperm cells. The zein proteins were also localized to protein storage vacuoles in a more dispersed pattern, suggesting that they were transported there after they were post-translationally sequestered into the ER.</description><subject>dap</subject><subject>days after pollination</subject><subject>endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - genetics</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Endoplasmic Reticulum - ultrastructure</subject><subject>ENDOSPERM</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Keywords: Endosperm — Maize — Protein-body — Transgenic — Tobacco — Zein</subject><subject>Microscopy, Electron</subject><subject>Multigene Family</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - growth & development</subject><subject>Nicotiana - metabolism</subject><subject>NICOTIANA TABACUM</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - growth & development</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>PROLAMINES</subject><subject>Protein Isoforms - biosynthesis</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Processing, Post-Translational - genetics</subject><subject>protein storage vacuole</subject><subject>PSV</subject><subject>rER</subject><subject>rough endoplasmic reticulum</subject><subject>Seeds - genetics</subject><subject>Seeds - metabolism</subject><subject>Seeds - ultrastructure</subject><subject>STORAGE PROTEINS</subject><subject>Transfection</subject><subject>TRANSGENIC PLANTS</subject><subject>Vacuoles - genetics</subject><subject>Vacuoles - metabolism</subject><subject>Vacuoles - ultrastructure</subject><subject>ZEA MAYS</subject><subject>Zea mays - genetics</subject><subject>Zea mays - metabolism</subject><subject>Zein - biosynthesis</subject><subject>Zein - genetics</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFrFTEQh4Mo9vn04l3JyYOwOtlskt2jFm2VB1aoIF5Ckp30pe7bbJNdaPvXm7IPPQozTCAfH8z8CHnJ4B2Djr-f3FR6z6B5RDasUazqQPDHZAPA6wpUy07Is5yvAcqbw1NywkTdCZDdhlxf7pEa55bDMpg5xJFGT80w7U11j2GkpeZkxnyFY3B0jrawkeLYxzxhOtCQaZ6NDUO4x57aO-pihbdTwpyPMovz6npOnngzZHxxnFvy4_Ony9Pzavft7Mvph13lGgZz1XKDsuwFqJTyHbSi88IywaXkHrB1Xllbi75XgnXS-FZBj3Xf9lZ548DyLXmzeqcUbxbMsz6E7HAYzIhxyVpKJduaN_8FWac62RV0S96uoEsx54ReTykcTLrTDPRDBLpEoNcICvz6aF3sAft_6PHmBahWIOQZb__-m_RbS8WV0Oc_f2n2cXfWfr-o9YPw1cp7E7W5SiHrrxc1gCjxskbwPwvim7E</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Coleman, C.E. (Brigham Young Univ., Utah (USA))</creator><creator>Yoho, P.R</creator><creator>Escobar, S</creator><creator>Ogawa, M</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040701</creationdate><title>The accumulation of alpha-zein in transgenic tobacco endosperm is stabilized by co-expression of beta-zein</title><author>Coleman, C.E. (Brigham Young Univ., Utah (USA)) ; Yoho, P.R ; Escobar, S ; Ogawa, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-83ae61090e777f90859f5b153663f0e8cf7bb25dd75196af870de2d8db7fac0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>dap</topic><topic>days after pollination</topic><topic>endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - genetics</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Endoplasmic Reticulum - ultrastructure</topic><topic>ENDOSPERM</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Keywords: Endosperm — Maize — Protein-body — Transgenic — Tobacco — Zein</topic><topic>Microscopy, Electron</topic><topic>Multigene Family</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - growth & development</topic><topic>Nicotiana - metabolism</topic><topic>NICOTIANA TABACUM</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - growth & development</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>PROLAMINES</topic><topic>Protein Isoforms - biosynthesis</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Processing, Post-Translational - genetics</topic><topic>protein storage vacuole</topic><topic>PSV</topic><topic>rER</topic><topic>rough endoplasmic reticulum</topic><topic>Seeds - genetics</topic><topic>Seeds - metabolism</topic><topic>Seeds - ultrastructure</topic><topic>STORAGE PROTEINS</topic><topic>Transfection</topic><topic>TRANSGENIC PLANTS</topic><topic>Vacuoles - genetics</topic><topic>Vacuoles - metabolism</topic><topic>Vacuoles - ultrastructure</topic><topic>ZEA MAYS</topic><topic>Zea mays - genetics</topic><topic>Zea mays - metabolism</topic><topic>Zein - biosynthesis</topic><topic>Zein - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coleman, C.E. (Brigham Young Univ., Utah (USA))</creatorcontrib><creatorcontrib>Yoho, P.R</creatorcontrib><creatorcontrib>Escobar, S</creatorcontrib><creatorcontrib>Ogawa, M</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coleman, C.E. (Brigham Young Univ., Utah (USA))</au><au>Yoho, P.R</au><au>Escobar, S</au><au>Ogawa, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The accumulation of alpha-zein in transgenic tobacco endosperm is stabilized by co-expression of beta-zein</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>45</volume><issue>7</issue><spage>864</spage><epage>871</epage><pages>864-871</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>The cysteine-poor alpha-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with delta-zein, whereas the hydrophobic cysteine-rich beta- and gamma-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressing zein genes, alpha-zein was unstable unless coexpressed with gamma-zein. Here we showed that alpha-zein was also stabilized by beta-zein. Small accretions of alpha- and beta-zeins, similar in appearance to maize protein bodies, were localized to the endoplasmic reticulum within tobacco endosperm cells. The zein proteins were also localized to protein storage vacuoles in a more dispersed pattern, suggesting that they were transported there after they were post-translationally sequestered into the ER.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>15295069</pmid><doi>10.1093/pcp/pch104</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | dap days after pollination endoplasmic reticulum Endoplasmic Reticulum - genetics Endoplasmic Reticulum - metabolism Endoplasmic Reticulum - ultrastructure ENDOSPERM Gene Expression Regulation, Plant - genetics Keywords: Endosperm — Maize — Protein-body — Transgenic — Tobacco — Zein Microscopy, Electron Multigene Family Nicotiana - genetics Nicotiana - growth & development Nicotiana - metabolism NICOTIANA TABACUM Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Plants, Genetically Modified - metabolism PROLAMINES Protein Isoforms - biosynthesis Protein Isoforms - genetics Protein Processing, Post-Translational - genetics protein storage vacuole PSV rER rough endoplasmic reticulum Seeds - genetics Seeds - metabolism Seeds - ultrastructure STORAGE PROTEINS Transfection TRANSGENIC PLANTS Vacuoles - genetics Vacuoles - metabolism Vacuoles - ultrastructure ZEA MAYS Zea mays - genetics Zea mays - metabolism Zein - biosynthesis Zein - genetics |
| title | The accumulation of alpha-zein in transgenic tobacco endosperm is stabilized by co-expression of beta-zein |
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