Study of peptide-sugar non-covalent complexes by infrared atmospheric pressure matrix-assisted laser desorption/ionization
Infrared atmospheric pressure matrix‐assisted laser desorption/ionization quadrupole ion trap mass spectrometry was applied to the study of siglec binding to oligosaccharide ligands. Peptides were designed to mimic the binding sites of three members of the siglec family: sialoadhesin, MAG and CD22....
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| Veröffentlicht in: | Journal of mass spectrometry. 2004-07, Vol.39 (7), p.736-742 |
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| container_end_page | 742 |
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| container_title | Journal of mass spectrometry. |
| container_volume | 39 |
| creator | Von Seggern, Christopher E. Cotter, Robert J. |
| description | Infrared atmospheric pressure matrix‐assisted laser desorption/ionization quadrupole ion trap mass spectrometry was applied to the study of siglec binding to oligosaccharide ligands. Peptides were designed to mimic the binding sites of three members of the siglec family: sialoadhesin, MAG and CD22. These peptides were tested for their ability to complex with their carbohydrate ligands 3′‐sialyllactose (3′SL) and 6′‐sialyllactose (6′SL). All peptides demonstrated the ability to bind to the carbohydrates, with the peptide representing sialoadhesin maintaining its binding specificity for 3′SL in preference to 6′SL. This technique can be used to study other protein–sugar interactions and can be expanded to create high‐throughput screening techniques. Copyright © 2004 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/jms.644 |
| format | Article |
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Peptides were designed to mimic the binding sites of three members of the siglec family: sialoadhesin, MAG and CD22. These peptides were tested for their ability to complex with their carbohydrate ligands 3′‐sialyllactose (3′SL) and 6′‐sialyllactose (6′SL). All peptides demonstrated the ability to bind to the carbohydrates, with the peptide representing sialoadhesin maintaining its binding specificity for 3′SL in preference to 6′SL. This technique can be used to study other protein–sugar interactions and can be expanded to create high‐throughput screening techniques. Copyright © 2004 John Wiley & Sons, Ltd.</description><identifier>ISSN: 1076-5174</identifier><identifier>EISSN: 1096-9888</identifier><identifier>DOI: 10.1002/jms.644</identifier><identifier>PMID: 15282752</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Atmospheric Pressure ; atmospheric pressure matrix-assisted laser desorption/ionization ; Binding Sites ; Biological and medical sciences ; carbohydrates ; Chemistry ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; infrared atmospheric pressure matrix-assisted laser desorption/ionization ; Interactions. Associations ; Intermolecular phenomena ; Lectins - chemistry ; Mass spectrometry ; Molecular biophysics ; non-covalent complexes ; Oligosaccharides - chemistry ; Organic chemistry ; Peptides - chemistry ; protein-carbohydrate binding ; Reactivity and mechanisms ; Sialic Acid Binding Immunoglobulin-like Lectins ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><ispartof>Journal of mass spectrometry., 2004-07, Vol.39 (7), p.736-742</ispartof><rights>Copyright © 2004 John Wiley & Sons, Ltd.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3814-2bf53b878f85335e59837ad7e762177222db8c61602e3336aeaa03db4008754a3</citedby><cites>FETCH-LOGICAL-c3814-2bf53b878f85335e59837ad7e762177222db8c61602e3336aeaa03db4008754a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjms.644$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjms.644$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27933,27934,45583,45584</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15958446$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15282752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Von Seggern, Christopher E.</creatorcontrib><creatorcontrib>Cotter, Robert J.</creatorcontrib><title>Study of peptide-sugar non-covalent complexes by infrared atmospheric pressure matrix-assisted laser desorption/ionization</title><title>Journal of mass spectrometry.</title><addtitle>J. Mass Spectrom</addtitle><description>Infrared atmospheric pressure matrix‐assisted laser desorption/ionization quadrupole ion trap mass spectrometry was applied to the study of siglec binding to oligosaccharide ligands. Peptides were designed to mimic the binding sites of three members of the siglec family: sialoadhesin, MAG and CD22. These peptides were tested for their ability to complex with their carbohydrate ligands 3′‐sialyllactose (3′SL) and 6′‐sialyllactose (6′SL). All peptides demonstrated the ability to bind to the carbohydrates, with the peptide representing sialoadhesin maintaining its binding specificity for 3′SL in preference to 6′SL. This technique can be used to study other protein–sugar interactions and can be expanded to create high‐throughput screening techniques. Copyright © 2004 John Wiley & Sons, Ltd.</description><subject>Atmospheric Pressure</subject><subject>atmospheric pressure matrix-assisted laser desorption/ionization</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>carbohydrates</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>infrared atmospheric pressure matrix-assisted laser desorption/ionization</subject><subject>Interactions. Associations</subject><subject>Intermolecular phenomena</subject><subject>Lectins - chemistry</subject><subject>Mass spectrometry</subject><subject>Molecular biophysics</subject><subject>non-covalent complexes</subject><subject>Oligosaccharides - chemistry</subject><subject>Organic chemistry</subject><subject>Peptides - chemistry</subject><subject>protein-carbohydrate binding</subject><subject>Reactivity and mechanisms</subject><subject>Sialic Acid Binding Immunoglobulin-like Lectins</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><issn>1076-5174</issn><issn>1096-9888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10E1v1DAQgOEIgWgpiH-AfAEOKK3j7xxRRQvVAhIF9Wg5zgRckjh4Etjtr8errIALB8tzeDQjvUXxtKKnFaXs7HbAUyXEveK4orUqa2PM_f2sVSkrLY6KR4i3lNK6FuphcVRJZpiW7Li4u56XdkdiRyaY5tBCictXl8gYx9LHn66HcSY-DlMPW0DS7EgYu-QStMTNQ8TpG6TgyZQAcUlABjensC0dYsA5o94hJNICxpTXx_Esv3Dn9uPj4kHneoQnh_-k-HLx5vP523Lz8fLd-etN6bmpRMmaTvLGaNMZybkEWRuuXatBK1ZpzRhrG-NVpSgDzrly4BzlbSMoNVoKx0-KF-veKcUfC-Bsh4Ae-t6NEBe0SmmVq4kMX67Qp4iYoLNTCoNLO1tRu89sc2abM2f57LByaQZo_7pD1wyeH4BD7_pcbPQB_3G1NEKo7F6t7lfoYfe_e_bq_fV6tlz1vu32j3bpu1Waa2lvPlzaTzdMXrDNlTX8N1pbpHI</recordid><startdate>200407</startdate><enddate>200407</enddate><creator>Von Seggern, Christopher E.</creator><creator>Cotter, Robert J.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200407</creationdate><title>Study of peptide-sugar non-covalent complexes by infrared atmospheric pressure matrix-assisted laser desorption/ionization</title><author>Von Seggern, Christopher E. ; Cotter, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3814-2bf53b878f85335e59837ad7e762177222db8c61602e3336aeaa03db4008754a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Atmospheric Pressure</topic><topic>atmospheric pressure matrix-assisted laser desorption/ionization</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>carbohydrates</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>infrared atmospheric pressure matrix-assisted laser desorption/ionization</topic><topic>Interactions. Associations</topic><topic>Intermolecular phenomena</topic><topic>Lectins - chemistry</topic><topic>Mass spectrometry</topic><topic>Molecular biophysics</topic><topic>non-covalent complexes</topic><topic>Oligosaccharides - chemistry</topic><topic>Organic chemistry</topic><topic>Peptides - chemistry</topic><topic>protein-carbohydrate binding</topic><topic>Reactivity and mechanisms</topic><topic>Sialic Acid Binding Immunoglobulin-like Lectins</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Von Seggern, Christopher E.</creatorcontrib><creatorcontrib>Cotter, Robert J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of mass spectrometry.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Von Seggern, Christopher E.</au><au>Cotter, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of peptide-sugar non-covalent complexes by infrared atmospheric pressure matrix-assisted laser desorption/ionization</atitle><jtitle>Journal of mass spectrometry.</jtitle><addtitle>J. Mass Spectrom</addtitle><date>2004-07</date><risdate>2004</risdate><volume>39</volume><issue>7</issue><spage>736</spage><epage>742</epage><pages>736-742</pages><issn>1076-5174</issn><eissn>1096-9888</eissn><abstract>Infrared atmospheric pressure matrix‐assisted laser desorption/ionization quadrupole ion trap mass spectrometry was applied to the study of siglec binding to oligosaccharide ligands. Peptides were designed to mimic the binding sites of three members of the siglec family: sialoadhesin, MAG and CD22. These peptides were tested for their ability to complex with their carbohydrate ligands 3′‐sialyllactose (3′SL) and 6′‐sialyllactose (6′SL). All peptides demonstrated the ability to bind to the carbohydrates, with the peptide representing sialoadhesin maintaining its binding specificity for 3′SL in preference to 6′SL. This technique can be used to study other protein–sugar interactions and can be expanded to create high‐throughput screening techniques. Copyright © 2004 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>15282752</pmid><doi>10.1002/jms.644</doi><tpages>7</tpages></addata></record> |
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| subjects | Atmospheric Pressure atmospheric pressure matrix-assisted laser desorption/ionization Binding Sites Biological and medical sciences carbohydrates Chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology infrared atmospheric pressure matrix-assisted laser desorption/ionization Interactions. Associations Intermolecular phenomena Lectins - chemistry Mass spectrometry Molecular biophysics non-covalent complexes Oligosaccharides - chemistry Organic chemistry Peptides - chemistry protein-carbohydrate binding Reactivity and mechanisms Sialic Acid Binding Immunoglobulin-like Lectins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods |
| title | Study of peptide-sugar non-covalent complexes by infrared atmospheric pressure matrix-assisted laser desorption/ionization |
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