The effect of cellular retinoic acid binding protein-I expression on the CYP26-mediated catabolism of all- trans retinoic acid and cell proliferation in head and neck squamous cell carcinoma
The aim of this study was to confirm if catabolism of all- trans retinoic acid (RA) is enhanced by type I cellular retinoic acid binding protein (CRABP-I) expression and to investigate the effect of this enhanced catabolism on cell proliferation of the head and neck squamous cell carcinoma (HNSCC) c...
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| Veröffentlicht in: | Metabolism, clinical and experimental clinical and experimental, 2004-08, Vol.53 (8), p.1007-1012 |
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| container_title | Metabolism, clinical and experimental |
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| creator | Won, Jun Yeon Nam, Eui-Cheol Yoo, Seung Joo Kwon, Hyun Ja Um, Soo Jong Han, Hye Sook Kim, Sun Hee Byun, Youngro Kim, Sang Yoon |
| description | The aim of this study was to confirm if catabolism of all-
trans retinoic acid (RA) is enhanced by type I cellular retinoic acid binding protein (CRABP-I) expression and to investigate the effect of this enhanced catabolism on cell proliferation of the head and neck squamous cell carcinoma (HNSCC) cell line, AMC-HN-7. We also analyzed the effects of CRABP-I on RA-induced retinoic acid receptor (RAR) activity. The expression of the CRABP-I in stably transfected AMC-HN-7 cell lines (HN7-BPIa and HN7-BPIb) resulted in a lower sensitivity to administered RA compared with that of controls in a clonogenic assay. HN7-BPIs cells showed an increased amount of polar metabolites of RA in thin-layer chromatography. The transcriptional activity of the reporter plasmid RARE(DR5)-tk-CAT after the treatment of RA was lesser in HN7-BPIs than in controls. These results suggest that the increased CYP26-mediated catabolism of RA by CRABP-I transfection might decrease the amount of RA that is accessible to the nuclear receptors and make HNSCC cells resistant to RA. |
| doi_str_mv | 10.1016/j.metabol.2003.12.015 |
| format | Article |
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trans retinoic acid (RA) is enhanced by type I cellular retinoic acid binding protein (CRABP-I) expression and to investigate the effect of this enhanced catabolism on cell proliferation of the head and neck squamous cell carcinoma (HNSCC) cell line, AMC-HN-7. We also analyzed the effects of CRABP-I on RA-induced retinoic acid receptor (RAR) activity. The expression of the CRABP-I in stably transfected AMC-HN-7 cell lines (HN7-BPIa and HN7-BPIb) resulted in a lower sensitivity to administered RA compared with that of controls in a clonogenic assay. HN7-BPIs cells showed an increased amount of polar metabolites of RA in thin-layer chromatography. The transcriptional activity of the reporter plasmid RARE(DR5)-tk-CAT after the treatment of RA was lesser in HN7-BPIs than in controls. These results suggest that the increased CYP26-mediated catabolism of RA by CRABP-I transfection might decrease the amount of RA that is accessible to the nuclear receptors and make HNSCC cells resistant to RA.</description><identifier>ISSN: 0026-0495</identifier><identifier>EISSN: 1532-8600</identifier><identifier>DOI: 10.1016/j.metabol.2003.12.015</identifier><identifier>PMID: 15281009</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Blotting, Western ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Cell Division - physiology ; Chloramphenicol O-Acetyltransferase - metabolism ; Chromatography, Thin Layer ; Cytochrome P-450 Enzyme System - metabolism ; Head and Neck Neoplasms - genetics ; Head and Neck Neoplasms - metabolism ; Head and Neck Neoplasms - pathology ; Humans ; Medical sciences ; Otorhinolaryngology (head neck, general aspects and miscellaneous) ; Otorhinolaryngology. Stomatology ; Receptors, Retinoic Acid - biosynthesis ; Receptors, Retinoic Acid - genetics ; Retinoic Acid 4-Hydroxylase ; Reverse Transcriptase Polymerase Chain Reaction ; Transfection ; Tretinoin - metabolism ; Tumor Stem Cell Assay ; Tumors</subject><ispartof>Metabolism, clinical and experimental, 2004-08, Vol.53 (8), p.1007-1012</ispartof><rights>2004 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c306t-47d045cd1ebcfb59c207caa4935203b953ebd9647434819cc266e040421d9fff3</citedby><cites>FETCH-LOGICAL-c306t-47d045cd1ebcfb59c207caa4935203b953ebd9647434819cc266e040421d9fff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.metabol.2003.12.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16066005$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15281009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Won, Jun Yeon</creatorcontrib><creatorcontrib>Nam, Eui-Cheol</creatorcontrib><creatorcontrib>Yoo, Seung Joo</creatorcontrib><creatorcontrib>Kwon, Hyun Ja</creatorcontrib><creatorcontrib>Um, Soo Jong</creatorcontrib><creatorcontrib>Han, Hye Sook</creatorcontrib><creatorcontrib>Kim, Sun Hee</creatorcontrib><creatorcontrib>Byun, Youngro</creatorcontrib><creatorcontrib>Kim, Sang Yoon</creatorcontrib><title>The effect of cellular retinoic acid binding protein-I expression on the CYP26-mediated catabolism of all- trans retinoic acid and cell proliferation in head and neck squamous cell carcinoma</title><title>Metabolism, clinical and experimental</title><addtitle>Metabolism</addtitle><description>The aim of this study was to confirm if catabolism of all-
trans retinoic acid (RA) is enhanced by type I cellular retinoic acid binding protein (CRABP-I) expression and to investigate the effect of this enhanced catabolism on cell proliferation of the head and neck squamous cell carcinoma (HNSCC) cell line, AMC-HN-7. We also analyzed the effects of CRABP-I on RA-induced retinoic acid receptor (RAR) activity. The expression of the CRABP-I in stably transfected AMC-HN-7 cell lines (HN7-BPIa and HN7-BPIb) resulted in a lower sensitivity to administered RA compared with that of controls in a clonogenic assay. HN7-BPIs cells showed an increased amount of polar metabolites of RA in thin-layer chromatography. The transcriptional activity of the reporter plasmid RARE(DR5)-tk-CAT after the treatment of RA was lesser in HN7-BPIs than in controls. These results suggest that the increased CYP26-mediated catabolism of RA by CRABP-I transfection might decrease the amount of RA that is accessible to the nuclear receptors and make HNSCC cells resistant to RA.</description><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Division - physiology</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>Chromatography, Thin Layer</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Head and Neck Neoplasms - genetics</subject><subject>Head and Neck Neoplasms - metabolism</subject><subject>Head and Neck Neoplasms - pathology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Otorhinolaryngology (head neck, general aspects and miscellaneous)</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Receptors, Retinoic Acid - biosynthesis</subject><subject>Receptors, Retinoic Acid - genetics</subject><subject>Retinoic Acid 4-Hydroxylase</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Transfection</subject><subject>Tretinoin - metabolism</subject><subject>Tumor Stem Cell Assay</subject><subject>Tumors</subject><issn>0026-0495</issn><issn>1532-8600</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGK1TAUhoMozp3RR1Cy0V3rSZqmtyuRy6gDA7oYF65Cmp44ubbpnSQd9OV8NtNpYcCNEMgif77zcz5CXjEoGTD57liOmHQ3DSUHqErGS2D1E7JjdcWLvQR4SnYAXBYg2vqMnMd4BICm2cvn5IzVfM8A2h35c3OLFK1Fk-hkqcFhmAcdaMDk_OQM1cb1tHO-d_4HPYUpofPFFcVfp4AxusnTfFKGHL5_zdNG7J1O2FOjH9q5OC5cPQwFTUH7-A9Z-_5h6IIenMWg08J0nt6iXp89mp803s16nOa4ho0OJkNG_YI8s3qI-HK7L8i3j5c3h8_F9ZdPV4cP14WpQKZCND2I2vQMO2O7ujUcGqO1aKuaQ9W1dYVd30rRiErsWWsMlxJBgOCsb6211QV5u3JzzbsZY1Kji0sV7TG3UlI2EvatyMF6DZowxRjQqlNwow6_FQO1iFNHtYlTizjFuMri8r_X24C5yzt8_LWZyoE3W0BHowebd2lcfMxJkFn6Anq_5jCv495hUNE49CZ7CVmy6if3nyp_AekzvBw</recordid><startdate>200408</startdate><enddate>200408</enddate><creator>Won, Jun Yeon</creator><creator>Nam, Eui-Cheol</creator><creator>Yoo, Seung Joo</creator><creator>Kwon, Hyun Ja</creator><creator>Um, Soo Jong</creator><creator>Han, Hye Sook</creator><creator>Kim, Sun Hee</creator><creator>Byun, Youngro</creator><creator>Kim, Sang Yoon</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200408</creationdate><title>The effect of cellular retinoic acid binding protein-I expression on the CYP26-mediated catabolism of all- trans retinoic acid and cell proliferation in head and neck squamous cell carcinoma</title><author>Won, Jun Yeon ; Nam, Eui-Cheol ; Yoo, Seung Joo ; Kwon, Hyun Ja ; Um, Soo Jong ; Han, Hye Sook ; Kim, Sun Hee ; Byun, Youngro ; Kim, Sang Yoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-47d045cd1ebcfb59c207caa4935203b953ebd9647434819cc266e040421d9fff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cell Division - physiology</topic><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>Chromatography, Thin Layer</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Head and Neck Neoplasms - genetics</topic><topic>Head and Neck Neoplasms - metabolism</topic><topic>Head and Neck Neoplasms - pathology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Otorhinolaryngology (head neck, general aspects and miscellaneous)</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Receptors, Retinoic Acid - biosynthesis</topic><topic>Receptors, Retinoic Acid - genetics</topic><topic>Retinoic Acid 4-Hydroxylase</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Transfection</topic><topic>Tretinoin - metabolism</topic><topic>Tumor Stem Cell Assay</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Won, Jun Yeon</creatorcontrib><creatorcontrib>Nam, Eui-Cheol</creatorcontrib><creatorcontrib>Yoo, Seung Joo</creatorcontrib><creatorcontrib>Kwon, Hyun Ja</creatorcontrib><creatorcontrib>Um, Soo Jong</creatorcontrib><creatorcontrib>Han, Hye Sook</creatorcontrib><creatorcontrib>Kim, Sun Hee</creatorcontrib><creatorcontrib>Byun, Youngro</creatorcontrib><creatorcontrib>Kim, Sang Yoon</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolism, clinical and experimental</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Won, Jun Yeon</au><au>Nam, Eui-Cheol</au><au>Yoo, Seung Joo</au><au>Kwon, Hyun Ja</au><au>Um, Soo Jong</au><au>Han, Hye Sook</au><au>Kim, Sun Hee</au><au>Byun, Youngro</au><au>Kim, Sang Yoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of cellular retinoic acid binding protein-I expression on the CYP26-mediated catabolism of all- trans retinoic acid and cell proliferation in head and neck squamous cell carcinoma</atitle><jtitle>Metabolism, clinical and experimental</jtitle><addtitle>Metabolism</addtitle><date>2004-08</date><risdate>2004</risdate><volume>53</volume><issue>8</issue><spage>1007</spage><epage>1012</epage><pages>1007-1012</pages><issn>0026-0495</issn><eissn>1532-8600</eissn><abstract>The aim of this study was to confirm if catabolism of all-
trans retinoic acid (RA) is enhanced by type I cellular retinoic acid binding protein (CRABP-I) expression and to investigate the effect of this enhanced catabolism on cell proliferation of the head and neck squamous cell carcinoma (HNSCC) cell line, AMC-HN-7. We also analyzed the effects of CRABP-I on RA-induced retinoic acid receptor (RAR) activity. The expression of the CRABP-I in stably transfected AMC-HN-7 cell lines (HN7-BPIa and HN7-BPIb) resulted in a lower sensitivity to administered RA compared with that of controls in a clonogenic assay. HN7-BPIs cells showed an increased amount of polar metabolites of RA in thin-layer chromatography. The transcriptional activity of the reporter plasmid RARE(DR5)-tk-CAT after the treatment of RA was lesser in HN7-BPIs than in controls. These results suggest that the increased CYP26-mediated catabolism of RA by CRABP-I transfection might decrease the amount of RA that is accessible to the nuclear receptors and make HNSCC cells resistant to RA.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>15281009</pmid><doi>10.1016/j.metabol.2003.12.015</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences Blotting, Western Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Cell Division - physiology Chloramphenicol O-Acetyltransferase - metabolism Chromatography, Thin Layer Cytochrome P-450 Enzyme System - metabolism Head and Neck Neoplasms - genetics Head and Neck Neoplasms - metabolism Head and Neck Neoplasms - pathology Humans Medical sciences Otorhinolaryngology (head neck, general aspects and miscellaneous) Otorhinolaryngology. Stomatology Receptors, Retinoic Acid - biosynthesis Receptors, Retinoic Acid - genetics Retinoic Acid 4-Hydroxylase Reverse Transcriptase Polymerase Chain Reaction Transfection Tretinoin - metabolism Tumor Stem Cell Assay Tumors |
| title | The effect of cellular retinoic acid binding protein-I expression on the CYP26-mediated catabolism of all- trans retinoic acid and cell proliferation in head and neck squamous cell carcinoma |
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