Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker
Purpose DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer. Methods RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using th...
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| Veröffentlicht in: | Journal of cancer research and clinical oncology 2009-02, Vol.135 (2), p.265-270 |
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| creator | Kim, Yun-Ji Huh, Jae-Won Kim, Dae-Soo Bae, Min-In Lee, Ja-Rang Ha, Hong-Seok Ahn, Kung Kim, Tae-Oh Song, Geun-Am Kim, Heui-Soo |
| description | Purpose DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer. Methods RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using the Image J program to compare the expression levels of each transcript. Results We found one of the transcripts was directly associated with an HERV-H LTR element that could be translated into protein sequence. Four new alternative transcripts were identified by RT-PCR analysis with various human tissue samples including 10 normal and adjacent tumor tissue sets. Semi-quantitative RT-PCR analysis showed the transcriptional activity of V3 and V2 was higher in tumor than in normal tissue samples, especially in the colorectal tissue samples. Conclusion Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer. |
| doi_str_mv | 10.1007/s00432-008-0445-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66759848</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20733266</sourcerecordid><originalsourceid>FETCH-LOGICAL-c520t-ec633d6d7476d2e1f7df051c3f19c14e1ca9546b50ceb5dfd807eb9edfabbd8c3</originalsourceid><addsrcrecordid>eNqFkU2P0zAQhi0EYsvCD-ACFhLcAjP-TI-ofEqLOMAKOFmOPe5mSZNipwf49bhKxUoc4GSN_czrd-Zl7CHCcwSwLwqAkqIBaBtQSjftLbbC4w1KqW-zFaDFRgs0Z-xeKddQa23FXXaGrcEWFa7Ylw_TQOEw-MzDlc8-zJT7X37up5FPic9XxF99-4ob5Fsaifsx8n4u3O_3Qx8WzNeSBz8Gyrzrp53P3ynfZ3eSHwo9OJ3n7PLN68-bd83Fx7fvNy8vmqAFzA0FI2U00SproiBMNibQGGTCdUBFGPxaK9NpCNTpmGILlro1xeS7LrZBnrNni-4-Tz8OVGa360ugYfAjTYfijLF63ar2v6AAK6UwpoJP_gKvp0Me6xBOiOoNlFEVwgUKeSolU3L73NfJfzoEd8zGLdm4mo07ZuOODh6dhA_djuJNxymMCjw9Ab4EP6Rcd9qXP5xAQKjrqpxYuFKfxi3lG4f_-v3x0pT85Pw2V-HLTwJQAmqrQKL8DX6KruI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>220510464</pqid></control><display><type>article</type><title>Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Kim, Yun-Ji ; Huh, Jae-Won ; Kim, Dae-Soo ; Bae, Min-In ; Lee, Ja-Rang ; Ha, Hong-Seok ; Ahn, Kung ; Kim, Tae-Oh ; Song, Geun-Am ; Kim, Heui-Soo</creator><creatorcontrib>Kim, Yun-Ji ; Huh, Jae-Won ; Kim, Dae-Soo ; Bae, Min-In ; Lee, Ja-Rang ; Ha, Hong-Seok ; Ahn, Kung ; Kim, Tae-Oh ; Song, Geun-Am ; Kim, Heui-Soo</creatorcontrib><description>Purpose DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer. Methods RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using the Image J program to compare the expression levels of each transcript. Results We found one of the transcripts was directly associated with an HERV-H LTR element that could be translated into protein sequence. Four new alternative transcripts were identified by RT-PCR analysis with various human tissue samples including 10 normal and adjacent tumor tissue sets. Semi-quantitative RT-PCR analysis showed the transcriptional activity of V3 and V2 was higher in tumor than in normal tissue samples, especially in the colorectal tissue samples. Conclusion Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s00432-008-0445-8</identifier><identifier>PMID: 18618141</identifier><identifier>CODEN: JCROD7</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Alternative Splicing ; Antineoplastic agents ; Biological and medical sciences ; Biomarkers ; Biomarkers, Tumor - genetics ; Cancer Research ; Colorectal cancer ; Colorectal Neoplasms - diagnosis ; Colorectal Neoplasms - genetics ; Disorders of higher nervous function. Focal brain diseases. Central vestibular syndrome and deafness. Brain stem syndromes ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genes ; Genetic Variation ; Hematology ; Humans ; Internal Medicine ; Male ; Medical sciences ; Medicine ; Medicine & Public Health ; Neoplasms - genetics ; Nerve Tissue Proteins - genetics ; Nervous system (semeiology, syndromes) ; Neurology ; Nuclear Proteins - genetics ; Oncology ; Original Paper ; Pharmacology. Drug treatments ; Pregnancy ; Reverse Transcriptase Polymerase Chain Reaction ; Transcription, Genetic</subject><ispartof>Journal of cancer research and clinical oncology, 2009-02, Vol.135 (2), p.265-270</ispartof><rights>Springer-Verlag 2008</rights><rights>2009 INIST-CNRS</rights><rights>Springer-Verlag 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-ec633d6d7476d2e1f7df051c3f19c14e1ca9546b50ceb5dfd807eb9edfabbd8c3</citedby><cites>FETCH-LOGICAL-c520t-ec633d6d7476d2e1f7df051c3f19c14e1ca9546b50ceb5dfd807eb9edfabbd8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00432-008-0445-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00432-008-0445-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21010633$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18618141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Yun-Ji</creatorcontrib><creatorcontrib>Huh, Jae-Won</creatorcontrib><creatorcontrib>Kim, Dae-Soo</creatorcontrib><creatorcontrib>Bae, Min-In</creatorcontrib><creatorcontrib>Lee, Ja-Rang</creatorcontrib><creatorcontrib>Ha, Hong-Seok</creatorcontrib><creatorcontrib>Ahn, Kung</creatorcontrib><creatorcontrib>Kim, Tae-Oh</creatorcontrib><creatorcontrib>Song, Geun-Am</creatorcontrib><creatorcontrib>Kim, Heui-Soo</creatorcontrib><title>Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><addtitle>J Cancer Res Clin Oncol</addtitle><description>Purpose DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer. Methods RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using the Image J program to compare the expression levels of each transcript. Results We found one of the transcripts was directly associated with an HERV-H LTR element that could be translated into protein sequence. Four new alternative transcripts were identified by RT-PCR analysis with various human tissue samples including 10 normal and adjacent tumor tissue sets. Semi-quantitative RT-PCR analysis showed the transcriptional activity of V3 and V2 was higher in tumor than in normal tissue samples, especially in the colorectal tissue samples. Conclusion Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer.</description><subject>Alternative Splicing</subject><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Cancer Research</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - diagnosis</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Disorders of higher nervous function. Focal brain diseases. Central vestibular syndrome and deafness. Brain stem syndromes</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes</subject><subject>Genetic Variation</subject><subject>Hematology</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neoplasms - genetics</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nervous system (semeiology, syndromes)</subject><subject>Neurology</subject><subject>Nuclear Proteins - genetics</subject><subject>Oncology</subject><subject>Original Paper</subject><subject>Pharmacology. Drug treatments</subject><subject>Pregnancy</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Transcription, Genetic</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU2P0zAQhi0EYsvCD-ACFhLcAjP-TI-ofEqLOMAKOFmOPe5mSZNipwf49bhKxUoc4GSN_czrd-Zl7CHCcwSwLwqAkqIBaBtQSjftLbbC4w1KqW-zFaDFRgs0Z-xeKddQa23FXXaGrcEWFa7Ylw_TQOEw-MzDlc8-zJT7X37up5FPic9XxF99-4ob5Fsaifsx8n4u3O_3Qx8WzNeSBz8Gyrzrp53P3ynfZ3eSHwo9OJ3n7PLN68-bd83Fx7fvNy8vmqAFzA0FI2U00SproiBMNibQGGTCdUBFGPxaK9NpCNTpmGILlro1xeS7LrZBnrNni-4-Tz8OVGa360ugYfAjTYfijLF63ar2v6AAK6UwpoJP_gKvp0Me6xBOiOoNlFEVwgUKeSolU3L73NfJfzoEd8zGLdm4mo07ZuOODh6dhA_djuJNxymMCjw9Ab4EP6Rcd9qXP5xAQKjrqpxYuFKfxi3lG4f_-v3x0pT85Pw2V-HLTwJQAmqrQKL8DX6KruI</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Kim, Yun-Ji</creator><creator>Huh, Jae-Won</creator><creator>Kim, Dae-Soo</creator><creator>Bae, Min-In</creator><creator>Lee, Ja-Rang</creator><creator>Ha, Hong-Seok</creator><creator>Ahn, Kung</creator><creator>Kim, Tae-Oh</creator><creator>Song, Geun-Am</creator><creator>Kim, Heui-Soo</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090201</creationdate><title>Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker</title><author>Kim, Yun-Ji ; Huh, Jae-Won ; Kim, Dae-Soo ; Bae, Min-In ; Lee, Ja-Rang ; Ha, Hong-Seok ; Ahn, Kung ; Kim, Tae-Oh ; Song, Geun-Am ; Kim, Heui-Soo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-ec633d6d7476d2e1f7df051c3f19c14e1ca9546b50ceb5dfd807eb9edfabbd8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alternative Splicing</topic><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Cancer Research</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - diagnosis</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Disorders of higher nervous function. Focal brain diseases. Central vestibular syndrome and deafness. Brain stem syndromes</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genes</topic><topic>Genetic Variation</topic><topic>Hematology</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neoplasms - genetics</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nervous system (semeiology, syndromes)</topic><topic>Neurology</topic><topic>Nuclear Proteins - genetics</topic><topic>Oncology</topic><topic>Original Paper</topic><topic>Pharmacology. Drug treatments</topic><topic>Pregnancy</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Yun-Ji</creatorcontrib><creatorcontrib>Huh, Jae-Won</creatorcontrib><creatorcontrib>Kim, Dae-Soo</creatorcontrib><creatorcontrib>Bae, Min-In</creatorcontrib><creatorcontrib>Lee, Ja-Rang</creatorcontrib><creatorcontrib>Ha, Hong-Seok</creatorcontrib><creatorcontrib>Ahn, Kung</creatorcontrib><creatorcontrib>Kim, Tae-Oh</creatorcontrib><creatorcontrib>Song, Geun-Am</creatorcontrib><creatorcontrib>Kim, Heui-Soo</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Yun-Ji</au><au>Huh, Jae-Won</au><au>Kim, Dae-Soo</au><au>Bae, Min-In</au><au>Lee, Ja-Rang</au><au>Ha, Hong-Seok</au><au>Ahn, Kung</au><au>Kim, Tae-Oh</au><au>Song, Geun-Am</au><au>Kim, Heui-Soo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><stitle>J Cancer Res Clin Oncol</stitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>135</volume><issue>2</issue><spage>265</spage><epage>270</epage><pages>265-270</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><coden>JCROD7</coden><abstract>Purpose DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer. Methods RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using the Image J program to compare the expression levels of each transcript. Results We found one of the transcripts was directly associated with an HERV-H LTR element that could be translated into protein sequence. Four new alternative transcripts were identified by RT-PCR analysis with various human tissue samples including 10 normal and adjacent tumor tissue sets. Semi-quantitative RT-PCR analysis showed the transcriptional activity of V3 and V2 was higher in tumor than in normal tissue samples, especially in the colorectal tissue samples. Conclusion Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>18618141</pmid><doi>10.1007/s00432-008-0445-8</doi><tpages>6</tpages></addata></record> |
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| subjects | Alternative Splicing Antineoplastic agents Biological and medical sciences Biomarkers Biomarkers, Tumor - genetics Cancer Research Colorectal cancer Colorectal Neoplasms - diagnosis Colorectal Neoplasms - genetics Disorders of higher nervous function. Focal brain diseases. Central vestibular syndrome and deafness. Brain stem syndromes Female Gene expression Gene Expression Regulation, Neoplastic Genes Genetic Variation Hematology Humans Internal Medicine Male Medical sciences Medicine Medicine & Public Health Neoplasms - genetics Nerve Tissue Proteins - genetics Nervous system (semeiology, syndromes) Neurology Nuclear Proteins - genetics Oncology Original Paper Pharmacology. Drug treatments Pregnancy Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic |
| title | Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker |
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