Functional characterization of recombinant batroxobin, a snake venom thrombin-like enzyme, expressed from Pichia pastoris

A thrombin-like enzyme of Bothrops atrox moojeni venom, batroxobin, specifically cleaves fibrinogen α chain, resulting in the formation of non-crosslinked fibrin clots. The cDNA encoding batroxobin was cloned, expressed in Pichia pastoris and the molecular function of purified recombinant protein wa...

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Veröffentlicht in:	FEBS letters 2004-07, Vol.571 (1), p.67-73
Hauptverfasser:	You, Weon-Kyoo, Choi, Won-Seok, Koh, You-Seok, Shin, Hang-Cheol, Jang, Yangsoo, Chung, Kwang-Hoe
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence APTT, activated partial thromboplastin time Batroxobin - chemistry Batroxobin - genetics Batroxobin - pharmacology Cloning, Molecular DNA, Complementary - genetics Fibrin clot Fibrinogen Molecular Sequence Data PAS, periodic acid-Schiff's reagent PBS, phosphate-buffered saline PCR, polymerase chain reaction Pichia - metabolism Pro-coagulant PT, prothrombin time Recombinant batroxobin Recombinant Proteins - chemistry Recombinant Proteins - pharmacology SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis Sequence Alignment Sequence Homology, Amino Acid Thrombin - chemistry Thrombin-like enzyme Trypsin - chemistry TT, thrombin time
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