The positive regulatory effect of TGF-beta2 on primitive murine hemopoietic stem and progenitor cells is dependent on age, genetic background, and serum factors
TGF-beta is considered a negative regulator of hemopoietic stem and progenitor cells. We have previously shown that one TGF-beta isoform, TGF-beta2, is, in fact, a positive regulator of murine hemopoietic stem cell function in vivo. In vitro, TGF-beta2, but not TGF-beta1 and TGF-beta3, had a biphasi...
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| Veröffentlicht in: | The Journal of immunology (1950) 2004-08, Vol.173 (4), p.2486-2493 |
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| container_title | The Journal of immunology (1950) |
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| creator | Henckaerts, Els Langer, Jessica C Orenstein, Jonathan Snoeck, Hans-Willem |
| description | TGF-beta is considered a negative regulator of hemopoietic stem and progenitor cells. We have previously shown that one TGF-beta isoform, TGF-beta2, is, in fact, a positive regulator of murine hemopoietic stem cell function in vivo. In vitro, TGF-beta2, but not TGF-beta1 and TGF-beta3, had a biphasic dose response on the proliferation of purified lin-Sca1(++)kit(+) (LSK) cells, with a stimulatory effect at low concentrations, which was subject to mouse strain-dependent variation. In this study we report that the stimulatory effect of TGF-beta2 on the proliferation of LSK cells increases with age and after replicative stress in C57BL/6, but not in DBA/2, mice. The age-related changes in the TGF-beta2 effect correlated with life span in BXD recombinant strains. The stimulatory effect of TGF-beta2 on the proliferation of LSK cells requires one or more nonprotein, low m.w. factors present in fetal calf and mouse sera. The activity of this factor(s) in mouse serum increases with age. Taken together, our data suggest a role for TGF-beta2 and as yet unknown serum factors in the aging of the hemopoietic stem cell compartment and possibly in organismal aging. |
| format | Article |
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We have previously shown that one TGF-beta isoform, TGF-beta2, is, in fact, a positive regulator of murine hemopoietic stem cell function in vivo. In vitro, TGF-beta2, but not TGF-beta1 and TGF-beta3, had a biphasic dose response on the proliferation of purified lin-Sca1(++)kit(+) (LSK) cells, with a stimulatory effect at low concentrations, which was subject to mouse strain-dependent variation. In this study we report that the stimulatory effect of TGF-beta2 on the proliferation of LSK cells increases with age and after replicative stress in C57BL/6, but not in DBA/2, mice. The age-related changes in the TGF-beta2 effect correlated with life span in BXD recombinant strains. The stimulatory effect of TGF-beta2 on the proliferation of LSK cells requires one or more nonprotein, low m.w. factors present in fetal calf and mouse sera. The activity of this factor(s) in mouse serum increases with age. Taken together, our data suggest a role for TGF-beta2 and as yet unknown serum factors in the aging of the hemopoietic stem cell compartment and possibly in organismal aging.</description><identifier>ISSN: 0022-1767</identifier><identifier>PMID: 15294963</identifier><language>eng</language><publisher>United States</publisher><subject>Aging ; Animals ; Bone Marrow Cells - cytology ; Bone Marrow Cells - immunology ; Dose-Response Relationship, Drug ; Genetic Linkage ; Hematopoiesis - genetics ; Hematopoietic Stem Cells - drug effects ; Hematopoietic Stem Cells - physiology ; Longevity ; Mice ; Serum - immunology ; Signal Transduction - drug effects ; Signal Transduction - immunology ; Transforming Growth Factor beta - immunology ; Transforming Growth Factor beta - pharmacology ; Transforming Growth Factor beta2</subject><ispartof>The Journal of immunology (1950), 2004-08, Vol.173 (4), p.2486-2493</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15294963$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Henckaerts, Els</creatorcontrib><creatorcontrib>Langer, Jessica C</creatorcontrib><creatorcontrib>Orenstein, Jonathan</creatorcontrib><creatorcontrib>Snoeck, Hans-Willem</creatorcontrib><title>The positive regulatory effect of TGF-beta2 on primitive murine hemopoietic stem and progenitor cells is dependent on age, genetic background, and serum factors</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>TGF-beta is considered a negative regulator of hemopoietic stem and progenitor cells. We have previously shown that one TGF-beta isoform, TGF-beta2, is, in fact, a positive regulator of murine hemopoietic stem cell function in vivo. In vitro, TGF-beta2, but not TGF-beta1 and TGF-beta3, had a biphasic dose response on the proliferation of purified lin-Sca1(++)kit(+) (LSK) cells, with a stimulatory effect at low concentrations, which was subject to mouse strain-dependent variation. In this study we report that the stimulatory effect of TGF-beta2 on the proliferation of LSK cells increases with age and after replicative stress in C57BL/6, but not in DBA/2, mice. The age-related changes in the TGF-beta2 effect correlated with life span in BXD recombinant strains. The stimulatory effect of TGF-beta2 on the proliferation of LSK cells requires one or more nonprotein, low m.w. factors present in fetal calf and mouse sera. The activity of this factor(s) in mouse serum increases with age. Taken together, our data suggest a role for TGF-beta2 and as yet unknown serum factors in the aging of the hemopoietic stem cell compartment and possibly in organismal aging.</description><subject>Aging</subject><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - immunology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Genetic Linkage</subject><subject>Hematopoiesis - genetics</subject><subject>Hematopoietic Stem Cells - drug effects</subject><subject>Hematopoietic Stem Cells - physiology</subject><subject>Longevity</subject><subject>Mice</subject><subject>Serum - immunology</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - immunology</subject><subject>Transforming Growth Factor beta - immunology</subject><subject>Transforming Growth Factor beta - pharmacology</subject><subject>Transforming Growth Factor beta2</subject><issn>0022-1767</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kL1OwzAUhTOAaCm8AroTUyPZsR03I6poQarEkj1ynOvU0NghdpD6Njwq6Q_TWb7z6ejcJHNCsiylMpez5D6ET0JITjJ-l8yoyApe5Gye_JZ7hN4HG-0PwoDteFDRD0dAY1BH8AbK7SatMaoMvIN-sN2F7cbBOoQ9dr73FqPVECJ2oFwzUb5FZycRaDwcAtgADfboGnTxpFEtLmFCzrVa6a928KNrlud2wGHswCg99cNDcmvUIeDjNRdJuXkt12_p7mP7vn7Zpb3gLK3rQgqkhjQ1Y7pgFIlkvDBMoM6pkByF4FyqVcOl5kRQtSo0pcbkfPrCSLZIni_aafr3iCFWnQ2n7cqhH0OV51JIQlYT-HQFx7rDpjodooZj9X8p-wOwrHPc</recordid><startdate>20040815</startdate><enddate>20040815</enddate><creator>Henckaerts, Els</creator><creator>Langer, Jessica C</creator><creator>Orenstein, Jonathan</creator><creator>Snoeck, Hans-Willem</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20040815</creationdate><title>The positive regulatory effect of TGF-beta2 on primitive murine hemopoietic stem and progenitor cells is dependent on age, genetic background, and serum factors</title><author>Henckaerts, Els ; Langer, Jessica C ; Orenstein, Jonathan ; Snoeck, Hans-Willem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p543-bb975e1f0db33c931e07349f35ec61574e55447a8d47c4051a89c11ff64152f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aging</topic><topic>Animals</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - immunology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Genetic Linkage</topic><topic>Hematopoiesis - genetics</topic><topic>Hematopoietic Stem Cells - drug effects</topic><topic>Hematopoietic Stem Cells - physiology</topic><topic>Longevity</topic><topic>Mice</topic><topic>Serum - immunology</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - immunology</topic><topic>Transforming Growth Factor beta - immunology</topic><topic>Transforming Growth Factor beta - pharmacology</topic><topic>Transforming Growth Factor beta2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Henckaerts, Els</creatorcontrib><creatorcontrib>Langer, Jessica C</creatorcontrib><creatorcontrib>Orenstein, Jonathan</creatorcontrib><creatorcontrib>Snoeck, Hans-Willem</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Henckaerts, Els</au><au>Langer, Jessica C</au><au>Orenstein, Jonathan</au><au>Snoeck, Hans-Willem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The positive regulatory effect of TGF-beta2 on primitive murine hemopoietic stem and progenitor cells is dependent on age, genetic background, and serum factors</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2004-08-15</date><risdate>2004</risdate><volume>173</volume><issue>4</issue><spage>2486</spage><epage>2493</epage><pages>2486-2493</pages><issn>0022-1767</issn><abstract>TGF-beta is considered a negative regulator of hemopoietic stem and progenitor cells. We have previously shown that one TGF-beta isoform, TGF-beta2, is, in fact, a positive regulator of murine hemopoietic stem cell function in vivo. In vitro, TGF-beta2, but not TGF-beta1 and TGF-beta3, had a biphasic dose response on the proliferation of purified lin-Sca1(++)kit(+) (LSK) cells, with a stimulatory effect at low concentrations, which was subject to mouse strain-dependent variation. In this study we report that the stimulatory effect of TGF-beta2 on the proliferation of LSK cells increases with age and after replicative stress in C57BL/6, but not in DBA/2, mice. The age-related changes in the TGF-beta2 effect correlated with life span in BXD recombinant strains. The stimulatory effect of TGF-beta2 on the proliferation of LSK cells requires one or more nonprotein, low m.w. factors present in fetal calf and mouse sera. The activity of this factor(s) in mouse serum increases with age. Taken together, our data suggest a role for TGF-beta2 and as yet unknown serum factors in the aging of the hemopoietic stem cell compartment and possibly in organismal aging.</abstract><cop>United States</cop><pmid>15294963</pmid><tpages>8</tpages></addata></record> |
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| ispartof | The Journal of immunology (1950), 2004-08, Vol.173 (4), p.2486-2493 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Aging Animals Bone Marrow Cells - cytology Bone Marrow Cells - immunology Dose-Response Relationship, Drug Genetic Linkage Hematopoiesis - genetics Hematopoietic Stem Cells - drug effects Hematopoietic Stem Cells - physiology Longevity Mice Serum - immunology Signal Transduction - drug effects Signal Transduction - immunology Transforming Growth Factor beta - immunology Transforming Growth Factor beta - pharmacology Transforming Growth Factor beta2 |
| title | The positive regulatory effect of TGF-beta2 on primitive murine hemopoietic stem and progenitor cells is dependent on age, genetic background, and serum factors |
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