A Force-Dependent Switch Reverses Type IV Pilus Retraction
Type IV pilus dynamics is important for virulence, motility, and DNA transfer in a wide variety of prokaryotes. The type IV pilus system constitutes a very robust and powerful molecular machine that transports pilus polymers as well as DNA through the bacterial cell envelope. In Neisseria gonorrhoea...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2004-07, Vol.101 (30), p.10961-10966 |
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Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Type IV pilus dynamics is important for virulence, motility, and DNA transfer in a wide variety of prokaryotes. The type IV pilus system constitutes a very robust and powerful molecular machine that transports pilus polymers as well as DNA through the bacterial cell envelope. In Neisseria gonorrhoeae, pilus retraction is a highly irreversible process that depends on PilT, an AAA ATPase family member. However when levels of PilT are reduced, the application of high external forces (F = 110 ± 10 pN) induces processive pilus elongation. At forces of >50 pN, single pili elongate at a rate of v = 350 ± 50 nm/s. For forces of |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.0402305101 |