Label-free determination of protein-surface interaction kinetics by ionic conductance inside a nanochannel
We have developed a label-free conductometric platform for the rapid measurement of kinetics parameters for the adsorption and desorption of proteins on surfaces. Adsorbed Bovine Serum Albumin (BSA) has been detected electrically with response times in the minute range, and kinetic models were elabo...
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| Veröffentlicht in: | Lab on a chip 2009-01, Vol.9 (2), p.319-324 |
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| container_title | Lab on a chip |
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| creator | Durand, Nicolas F Y Renaud, Philippe |
| description | We have developed a label-free conductometric platform for the rapid measurement of kinetics parameters for the adsorption and desorption of proteins on surfaces. Adsorbed Bovine Serum Albumin (BSA) has been detected electrically with response times in the minute range, and kinetic models were elaborated and compared to the measurements. The device presents similar characteristics to Surface Plasmon Resonance (SPR) immunosensors, but takes advantage of a simpler, low-cost electronic measurement unit. |
| doi_str_mv | 10.1039/b811006a |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1473-0197 |
| ispartof | Lab on a chip, 2009-01, Vol.9 (2), p.319-324 |
| issn | 1473-0197 1473-0189 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66752202 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Adsorption Animals Biosensing Techniques - instrumentation Biosensing Techniques - methods Cattle Electric Conductivity Hydrogen-Ion Concentration Kinetics Nanostructures - chemistry Osmolar Concentration Proteins - chemistry Serum Albumin, Bovine - chemistry Surface Properties |
| title | Label-free determination of protein-surface interaction kinetics by ionic conductance inside a nanochannel |
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