Age-related and regional differences in secretin and secretin receptor mRNA levels in the rat brain
In the present study expression levels of secretin and secretin receptor mRNAs in several brain regions of rats ranging in age from postnatal days 7 to 60 were investigated by quantitative real-time PCR. Expression of secretin and secretin receptor was detected in the central amygdala, hippocampus,...
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| Veröffentlicht in: | Neuroscience letters 2004-08, Vol.366 (2), p.176-181 |
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| creator | Tay, Joyce Goulet, Martin Rusche, James Boismenu, Richard |
| description | In the present study expression levels of secretin and secretin receptor mRNAs in several brain regions of rats ranging in age from postnatal days 7 to 60 were investigated by quantitative real-time PCR. Expression of secretin and secretin receptor was detected in the central amygdala, hippocampus, area postrema, nucleus of the tractus solitary and cerebellum. The cerebellum expressed secretin receptor at significantly higher levels than that found in other brain regions within all the ages examined. In contrast, secretin mRNA was significantly higher in the nucleus of the tractus solitary than in the other four brain regions examined in postnatal day-21, -30 and -60 rats. Within most brain regions, both secretin and secretin receptor mRNAs were more abundant in postnatal day-7 and -14 rats as compared to postnatal day-21, -30 and -60 rats. Thus, secretin and its receptor are widely expressed in rat brain and the expression of both genes is developmentally regulated during the first few weeks following birth. |
| doi_str_mv | 10.1016/j.neulet.2004.05.030 |
| format | Article |
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Expression of secretin and secretin receptor was detected in the central amygdala, hippocampus, area postrema, nucleus of the tractus solitary and cerebellum. The cerebellum expressed secretin receptor at significantly higher levels than that found in other brain regions within all the ages examined. In contrast, secretin mRNA was significantly higher in the nucleus of the tractus solitary than in the other four brain regions examined in postnatal day-21, -30 and -60 rats. Within most brain regions, both secretin and secretin receptor mRNAs were more abundant in postnatal day-7 and -14 rats as compared to postnatal day-21, -30 and -60 rats. 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Expression of secretin and secretin receptor was detected in the central amygdala, hippocampus, area postrema, nucleus of the tractus solitary and cerebellum. The cerebellum expressed secretin receptor at significantly higher levels than that found in other brain regions within all the ages examined. In contrast, secretin mRNA was significantly higher in the nucleus of the tractus solitary than in the other four brain regions examined in postnatal day-21, -30 and -60 rats. Within most brain regions, both secretin and secretin receptor mRNAs were more abundant in postnatal day-7 and -14 rats as compared to postnatal day-21, -30 and -60 rats. 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Psychology</subject><subject>G-protein coupled receptors</subject><subject>Gene expression</subject><subject>Male</subject><subject>Peptides</subject><subject>Quantitative real-time TaqMan PCR analysis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, G-Protein-Coupled</subject><subject>Receptors, Gastrointestinal Hormone - biosynthesis</subject><subject>Receptors, Gastrointestinal Hormone - genetics</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Secretin - biosynthesis</subject><subject>Secretin - genetics</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEuLFDEQgIO4uOPqPxDJRW_dVtJJp_siDIsvWHZB9BzSSWXNkOkek8zC_nvTzqCePNWDr4qqj5BXDFoGrH-3a2c8RiwtBxAtyBY6eEI2bFC8UaPiT8mmdkTTjQIuyfOcdwAgmRTPyCWTXPVc8A2x23tsEkZT0FEzO5rwPiyzidQF7zHhbDHTMNOMNmGpyQr9KRJaPJQl0f3X2y2N-IDxN11-IE2m0CmZML8gF97EjC_P8Yp8__jh2_Xn5ubu05fr7U1jhRSlGYbRcWGnEf04OAFuch461fXSWa9sbxRDnIzqRoaTVB5H1oEZcKgth5x3V-Ttae8hLT-PmIveh2wxRjPjcsy671X9X8kKihNo05JzQq8PKexNetQM9CpX7_RJrl7lapC6qqxjr8_7j9Me3d-hs80KvDkDJlsTfTKzDfkfbuxFDyv3_sRVW_gQMOlsw2rahSq0aLeE_1_yCz7lmtE</recordid><startdate>20040812</startdate><enddate>20040812</enddate><creator>Tay, Joyce</creator><creator>Goulet, Martin</creator><creator>Rusche, James</creator><creator>Boismenu, Richard</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040812</creationdate><title>Age-related and regional differences in secretin and secretin receptor mRNA levels in the rat brain</title><author>Tay, Joyce ; Goulet, Martin ; Rusche, James ; Boismenu, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-889d24cb9ef98d40dbdf037365dcf7c6a71eeba7391eb57fe9130a8e8a73de223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Brain - anatomy & histology</topic><topic>Brain - growth & development</topic><topic>Brain - metabolism</topic><topic>Development</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>G-protein coupled receptors</topic><topic>Gene expression</topic><topic>Male</topic><topic>Peptides</topic><topic>Quantitative real-time TaqMan PCR analysis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, G-Protein-Coupled</topic><topic>Receptors, Gastrointestinal Hormone - biosynthesis</topic><topic>Receptors, Gastrointestinal Hormone - genetics</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Secretin - biosynthesis</topic><topic>Secretin - genetics</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tay, Joyce</creatorcontrib><creatorcontrib>Goulet, Martin</creatorcontrib><creatorcontrib>Rusche, James</creatorcontrib><creatorcontrib>Boismenu, Richard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tay, Joyce</au><au>Goulet, Martin</au><au>Rusche, James</au><au>Boismenu, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-related and regional differences in secretin and secretin receptor mRNA levels in the rat brain</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2004-08-12</date><risdate>2004</risdate><volume>366</volume><issue>2</issue><spage>176</spage><epage>181</epage><pages>176-181</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>In the present study expression levels of secretin and secretin receptor mRNAs in several brain regions of rats ranging in age from postnatal days 7 to 60 were investigated by quantitative real-time PCR. 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| ispartof | Neuroscience letters, 2004-08, Vol.366 (2), p.176-181 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Animals, Newborn Biological and medical sciences Brain - anatomy & histology Brain - growth & development Brain - metabolism Development Fundamental and applied biological sciences. Psychology G-protein coupled receptors Gene expression Male Peptides Quantitative real-time TaqMan PCR analysis Rats Rats, Sprague-Dawley Receptors, G-Protein-Coupled Receptors, Gastrointestinal Hormone - biosynthesis Receptors, Gastrointestinal Hormone - genetics RNA, Messenger - biosynthesis Secretin - biosynthesis Secretin - genetics Vertebrates: nervous system and sense organs |
| title | Age-related and regional differences in secretin and secretin receptor mRNA levels in the rat brain |
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