Characterization of midgut trypsinogen-like cDNA and enzymatic activity in Plutella xylostella parasitized by Cotesia vestalis or Diadegma semiclausum

Protein digestion in insects is a result of the action of a complex of proteinases present in the midgut. In this report we describe the cloning and sequencing of a trypsin cDNA from larvae of the lepidopteran herbivore Plutella xylostella. We investigated the expression of this gene and enzymatic a...

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Veröffentlicht in:	Archives of insect biochemistry and physiology 2009, Vol.70 (1), p.3-17
Hauptverfasser:	Shi, Min, Huang, Fang, Chen, Ya-Feng, Meng, Xiang-Feng, Chen, Xue-Xin
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Animals BApNAase Base Sequence cDNA sequence complementary DNA Cotesia Cotesia vestalis Diadegma Diadegma semiclausum Digestive System - enzymology DNA, Complementary - genetics DNA, Complementary - metabolism enzyme activity gene expression Gene Expression Regulation - physiology Host-Parasite Interactions Hydrogen-Ion Concentration Larva - metabolism Larva - parasitology Lepidoptera messenger RNA midgut molecular cloning Molecular Sequence Data Moths - parasitology mRNA transcripts nucleotide sequences parasitism parasitoids Phylogeny Plutella Plutella xylostella RNA, Messenger - genetics RNA, Messenger - metabolism sequence analysis Trypsin - metabolism trypsinogen Trypsinogen - metabolism trypsinogen-like proteinases Wasps - physiology
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