Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: Preliminary study
In cystic fibrosis (CF) children, we investigated the predictive impact of glutathione S-transferases (GST) activity and genotypes P1, M1 and T1, and antioxidant levels on stage-severity of Pseudomonas aeruginosa lung infection. GST activity was determined in whole blood by spectrophotometry, and GS...
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| Veröffentlicht in: | Clinical biochemistry 2009, Vol.42 (1), p.57-63 |
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| creator | Feuillet-Fieux, Marie-Noëlle Nguyen-Khoa, Thao Loriot, Marie-Anne Kelly, Mairead de Villartay, Philippe Sermet, Isabelle Verrier, Patricia Bonnefont, Jean-Paul Beaune, Philippe Lenoir, Gérard Lacour, Bernard |
| description | In cystic fibrosis (CF) children, we investigated the predictive impact of glutathione S-transferases (GST) activity and genotypes P1, M1 and T1, and antioxidant levels on stage-severity of
Pseudomonas aeruginosa lung infection.
GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC.
No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%).
GST activity and genotype could play an important role in modulating
P. aeruginosa lung infection in CF patients. |
| doi_str_mv | 10.1016/j.clinbiochem.2008.09.116 |
| format | Article |
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Pseudomonas aeruginosa lung infection.
GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC.
No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%).
GST activity and genotype could play an important role in modulating
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Pseudomonas aeruginosa lung infection.
GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC.
No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%).
GST activity and genotype could play an important role in modulating
P. aeruginosa lung infection in CF patients.</description><subject>Adolescent</subject><subject>Ascorbic Acid - blood</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cystic fibrosis</subject><subject>Cystic Fibrosis - enzymology</subject><subject>Cystic Fibrosis - microbiology</subject><subject>Erythrocytes - metabolism</subject><subject>Female</subject><subject>Genotype</subject><subject>Glutathione - metabolism</subject><subject>Glutathione S-transferase</subject><subject>Glutathione Transferase - genetics</subject><subject>Glutathione Transferase - metabolism</subject><subject>Humans</subject><subject>Lung infection</subject><subject>Male</subject><subject>Oxidative stress</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - pathogenicity</subject><subject>Pseudomonas Infections - physiopathology</subject><subject>Vitamin A - blood</subject><subject>Vitamin E - blood</subject><subject>Young Adult</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1r3DAQhkVoSbZp_0JQL73Z0YctS7mVJU0DgQaau5ClUVaLLSeSXNh_X4VdaG_tSSN43hlmHoQ-U9JSQsX1vrVTiGNY7A7mlhEiW6JaSsUZ2lA58IYpzt-hDSFENYoycoE-5LyvX9ZJcY4uqFSDEF2_Qa9301pM2YUlAv7ZlGRi9pBMhowTTKaAw2XBjy02kNbnEJds8LTGZxyiB1tqrlbYHnIJFvswpiWHjO0uTC5BvMGPtUuYQzTpgHNZ3eEjeu_NlOHT6b1ET99un7bfm4cfd_fbrw-N7TgtTa-cHA23vfeUcALg3OCAStv1TlnwrHe8syBHaxkZuAQvJLPS0QGAOsYv0Zdj25e0vK6Qi55DtjBNJsKyZi3E0HWC_BtkhPOedEMF1RG0dcecwOuXFOa6l6ZEv3nRe_2XF_3mRROlq5eavToNWccZ3J_kSUQFtkcA6kl-BUg62wDRggupnlm7JfzHmN-o16gF</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Feuillet-Fieux, Marie-Noëlle</creator><creator>Nguyen-Khoa, Thao</creator><creator>Loriot, Marie-Anne</creator><creator>Kelly, Mairead</creator><creator>de Villartay, Philippe</creator><creator>Sermet, Isabelle</creator><creator>Verrier, Patricia</creator><creator>Bonnefont, Jean-Paul</creator><creator>Beaune, Philippe</creator><creator>Lenoir, Gérard</creator><creator>Lacour, Bernard</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>2009</creationdate><title>Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: Preliminary study</title><author>Feuillet-Fieux, Marie-Noëlle ; 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Pseudomonas aeruginosa lung infection.
GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC.
No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%).
GST activity and genotype could play an important role in modulating
P. aeruginosa lung infection in CF patients.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18976645</pmid><doi>10.1016/j.clinbiochem.2008.09.116</doi><tpages>7</tpages></addata></record> |
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| subjects | Adolescent Ascorbic Acid - blood Child Child, Preschool Chromatography, High Pressure Liquid Cystic fibrosis Cystic Fibrosis - enzymology Cystic Fibrosis - microbiology Erythrocytes - metabolism Female Genotype Glutathione - metabolism Glutathione S-transferase Glutathione Transferase - genetics Glutathione Transferase - metabolism Humans Lung infection Male Oxidative stress Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Pseudomonas aeruginosa Pseudomonas aeruginosa - pathogenicity Pseudomonas Infections - physiopathology Vitamin A - blood Vitamin E - blood Young Adult |
| title | Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: Preliminary study |
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