Photomorphogenesis in the hypogeous fungus Tuber borchii: isolation and characterization of Tbwc-1, the homologue of the blue-light photoreceptor of Neurospora crassa
Truffles form a group of plant–symbiotic Ascomycetes whose hypogeous life cycle is poorly understood. Here we present initial evidence for the influence of light on Tuber borchii mycelial growth and the identification and cloning of a gene, Tbwc-1, homologous to a blue-light photoreceptor of Neurosp...
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| Veröffentlicht in: | Fungal genetics and biology 2004-07, Vol.41 (7), p.688-697 |
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| creator | Ambra, R. Grimaldi, B. Zamboni, S. Filetici, P. Macino, G. Ballario, P. |
| description | Truffles form a group of plant–symbiotic Ascomycetes whose hypogeous life cycle is poorly understood. Here we present initial evidence for the influence of light on
Tuber borchii mycelial growth and the identification and cloning of a gene,
Tbwc-1, homologous to a blue-light photoreceptor of
Neurospora crassa. Blue-light irradiation of
T. borchii colonies inhibits their apical growth. It also alters apical growth in
N. crassa. In
Neurospora, the response is controlled by a nuclear photoreceptor, NcWC-1 (White Collar-1), which consists of a sensor domain (LOV) and a transcriptional factor moiety. We isolated a gene (
Tbwc-1) whose deduced amino acid sequence shows a high similarity and colinearity of domains with NcWC-1, except for the polyglutamine regions. As previously found in
Neurospora,
Tbwc-1 mRNA is under light control and its steady state level increases upon irradiation. In silico analysis of the TbWC-1 sensor domain (LOV) supports the hypothesis that TbWC-1 is a photoreceptor, while the absence of the two polyglutamine regions involved in transcriptional activation in
Neurospora suggests that this function in
Tuber could be lost. |
| doi_str_mv | 10.1016/j.fgb.2004.02.004 |
| format | Article |
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Tuber borchii mycelial growth and the identification and cloning of a gene,
Tbwc-1, homologous to a blue-light photoreceptor of
Neurospora crassa. Blue-light irradiation of
T. borchii colonies inhibits their apical growth. It also alters apical growth in
N. crassa. In
Neurospora, the response is controlled by a nuclear photoreceptor, NcWC-1 (White Collar-1), which consists of a sensor domain (LOV) and a transcriptional factor moiety. We isolated a gene (
Tbwc-1) whose deduced amino acid sequence shows a high similarity and colinearity of domains with NcWC-1, except for the polyglutamine regions. As previously found in
Neurospora,
Tbwc-1 mRNA is under light control and its steady state level increases upon irradiation. In silico analysis of the TbWC-1 sensor domain (LOV) supports the hypothesis that TbWC-1 is a photoreceptor, while the absence of the two polyglutamine regions involved in transcriptional activation in
Neurospora suggests that this function in
Tuber could be lost.</description><identifier>ISSN: 1087-1845</identifier><identifier>EISSN: 1096-0937</identifier><identifier>DOI: 10.1016/j.fgb.2004.02.004</identifier><identifier>PMID: 15275664</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject><![CDATA[Amino Acid Sequence ; Ascomycota - cytology ; Ascomycota - genetics ; Ascomycota - growth & development ; Blue light ; Cloning, Molecular ; Conserved Sequence ; DNA, Fungal - chemistry ; DNA, Fungal - isolation & purification ; DNA-Binding Proteins - chemistry ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - isolation & purification ; Fungal Proteins - chemistry ; Fungal Proteins - genetics ; Fungal Proteins - isolation & purification ; Gene Expression Regulation, Fungal ; Genes, Fungal ; Hypogeous life ; Light ; Models, Molecular ; Molecular Sequence Data ; Morphogenesis ; Mycelium - genetics ; Mycelium - growth & development ; Neurospora crassa - genetics ; Photomorphogenesis ; Photoreceptor ; Photoreceptors, Microbial - genetics ; Photoreceptors, Microbial - isolation & purification ; Polyglutamic Acid - genetics ; Protein Structure, Tertiary ; RNA, Fungal - analysis ; RNA, Messenger - analysis ; Sequence Alignment ; Sequence Homology, Amino Acid ; Signal transduction ; Transcription Factors - chemistry ; Transcription Factors - genetics ; Transcription Factors - isolation & purification ; Transcription, Genetic ; Tuber borchii ; White collar-1]]></subject><ispartof>Fungal genetics and biology, 2004-07, Vol.41 (7), p.688-697</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-b7f2858c7f9eda343074790c5abaf9f740d9323fff4808fb1f036227c2426c343</citedby><cites>FETCH-LOGICAL-c470t-b7f2858c7f9eda343074790c5abaf9f740d9323fff4808fb1f036227c2426c343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fgb.2004.02.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15275664$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ambra, R.</creatorcontrib><creatorcontrib>Grimaldi, B.</creatorcontrib><creatorcontrib>Zamboni, S.</creatorcontrib><creatorcontrib>Filetici, P.</creatorcontrib><creatorcontrib>Macino, G.</creatorcontrib><creatorcontrib>Ballario, P.</creatorcontrib><title>Photomorphogenesis in the hypogeous fungus Tuber borchii: isolation and characterization of Tbwc-1, the homologue of the blue-light photoreceptor of Neurospora crassa</title><title>Fungal genetics and biology</title><addtitle>Fungal Genet Biol</addtitle><description>Truffles form a group of plant–symbiotic Ascomycetes whose hypogeous life cycle is poorly understood. Here we present initial evidence for the influence of light on
Tuber borchii mycelial growth and the identification and cloning of a gene,
Tbwc-1, homologous to a blue-light photoreceptor of
Neurospora crassa. Blue-light irradiation of
T. borchii colonies inhibits their apical growth. It also alters apical growth in
N. crassa. In
Neurospora, the response is controlled by a nuclear photoreceptor, NcWC-1 (White Collar-1), which consists of a sensor domain (LOV) and a transcriptional factor moiety. We isolated a gene (
Tbwc-1) whose deduced amino acid sequence shows a high similarity and colinearity of domains with NcWC-1, except for the polyglutamine regions. As previously found in
Neurospora,
Tbwc-1 mRNA is under light control and its steady state level increases upon irradiation. In silico analysis of the TbWC-1 sensor domain (LOV) supports the hypothesis that TbWC-1 is a photoreceptor, while the absence of the two polyglutamine regions involved in transcriptional activation in
Neurospora suggests that this function in
Tuber could be lost.</description><subject>Amino Acid Sequence</subject><subject>Ascomycota - cytology</subject><subject>Ascomycota - genetics</subject><subject>Ascomycota - growth & development</subject><subject>Blue light</subject><subject>Cloning, Molecular</subject><subject>Conserved Sequence</subject><subject>DNA, Fungal - chemistry</subject><subject>DNA, Fungal - isolation & purification</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - isolation & purification</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - isolation & purification</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Fungal</subject><subject>Hypogeous life</subject><subject>Light</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Morphogenesis</subject><subject>Mycelium - genetics</subject><subject>Mycelium - growth & development</subject><subject>Neurospora crassa - genetics</subject><subject>Photomorphogenesis</subject><subject>Photoreceptor</subject><subject>Photoreceptors, Microbial - genetics</subject><subject>Photoreceptors, Microbial - isolation & purification</subject><subject>Polyglutamic Acid - genetics</subject><subject>Protein Structure, Tertiary</subject><subject>RNA, Fungal - analysis</subject><subject>RNA, Messenger - analysis</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal transduction</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - isolation & purification</subject><subject>Transcription, Genetic</subject><subject>Tuber borchii</subject><subject>White collar-1</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRSMEYoaBD2ADXrEioew4dgIrNOIljQCJnrXlOOXErXQc7AQ0fBDfiaO0xA5W16o69XDdLHtKoaBAxatjYfu2YAC8AFYkuZddUmhEDk0p72_vWua05tVF9ijGIwClFacPswtaMVkJwS-z318Hv_iTD_Pge5wwukjcRJYByXA3p5BfI7Hr1Cc5rC0G0vpgBudeExf9qBfnJ6KnjphBB20WDO7XHvSWHNqfJqcv925pyOj7FbfEFmjHFfPR9cNC5m2HgAbnJFv-M67Bx9kHTUzQMerH2QOrx4hPznqV3b5_d7j-mN98-fDp-u1NbriEJW-lZXVVG2kb7HTJS5BcNmAq3WrbWMmha0pWWmt5DbVtqYVSMCYN40yYxF9lL_a-c_DfV4yLOrlocBz1tF1CCSE5LxvxX5BKJiVjTQLpDpr0oxjQqjm4kw53ioLaXFRHlVxUm4sKmEqSap6dm6_tCbu_FWfbEvB8B6z2SvfBRXX7jQEtAZqGU0ET8WYnMF3rh8OgonE4GexcOvSiOu_-scAf4DK5qw</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Ambra, R.</creator><creator>Grimaldi, B.</creator><creator>Zamboni, S.</creator><creator>Filetici, P.</creator><creator>Macino, G.</creator><creator>Ballario, P.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040701</creationdate><title>Photomorphogenesis in the hypogeous fungus Tuber borchii: isolation and characterization of Tbwc-1, the homologue of the blue-light photoreceptor of Neurospora crassa</title><author>Ambra, R. ; Grimaldi, B. ; Zamboni, S. ; Filetici, P. ; Macino, G. ; Ballario, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-b7f2858c7f9eda343074790c5abaf9f740d9323fff4808fb1f036227c2426c343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Ascomycota - cytology</topic><topic>Ascomycota - genetics</topic><topic>Ascomycota - growth & development</topic><topic>Blue light</topic><topic>Cloning, Molecular</topic><topic>Conserved Sequence</topic><topic>DNA, Fungal - chemistry</topic><topic>DNA, Fungal - isolation & purification</topic><topic>DNA-Binding Proteins - chemistry</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - isolation & purification</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - isolation & purification</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Fungal</topic><topic>Hypogeous life</topic><topic>Light</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Morphogenesis</topic><topic>Mycelium - genetics</topic><topic>Mycelium - growth & development</topic><topic>Neurospora crassa - genetics</topic><topic>Photomorphogenesis</topic><topic>Photoreceptor</topic><topic>Photoreceptors, Microbial - genetics</topic><topic>Photoreceptors, Microbial - isolation & purification</topic><topic>Polyglutamic Acid - genetics</topic><topic>Protein Structure, Tertiary</topic><topic>RNA, Fungal - analysis</topic><topic>RNA, Messenger - analysis</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Signal transduction</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - isolation & purification</topic><topic>Transcription, Genetic</topic><topic>Tuber borchii</topic><topic>White collar-1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ambra, R.</creatorcontrib><creatorcontrib>Grimaldi, B.</creatorcontrib><creatorcontrib>Zamboni, S.</creatorcontrib><creatorcontrib>Filetici, P.</creatorcontrib><creatorcontrib>Macino, G.</creatorcontrib><creatorcontrib>Ballario, P.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fungal genetics and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ambra, R.</au><au>Grimaldi, B.</au><au>Zamboni, S.</au><au>Filetici, P.</au><au>Macino, G.</au><au>Ballario, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photomorphogenesis in the hypogeous fungus Tuber borchii: isolation and characterization of Tbwc-1, the homologue of the blue-light photoreceptor of Neurospora crassa</atitle><jtitle>Fungal genetics and biology</jtitle><addtitle>Fungal Genet Biol</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>41</volume><issue>7</issue><spage>688</spage><epage>697</epage><pages>688-697</pages><issn>1087-1845</issn><eissn>1096-0937</eissn><abstract>Truffles form a group of plant–symbiotic Ascomycetes whose hypogeous life cycle is poorly understood. Here we present initial evidence for the influence of light on
Tuber borchii mycelial growth and the identification and cloning of a gene,
Tbwc-1, homologous to a blue-light photoreceptor of
Neurospora crassa. Blue-light irradiation of
T. borchii colonies inhibits their apical growth. It also alters apical growth in
N. crassa. In
Neurospora, the response is controlled by a nuclear photoreceptor, NcWC-1 (White Collar-1), which consists of a sensor domain (LOV) and a transcriptional factor moiety. We isolated a gene (
Tbwc-1) whose deduced amino acid sequence shows a high similarity and colinearity of domains with NcWC-1, except for the polyglutamine regions. As previously found in
Neurospora,
Tbwc-1 mRNA is under light control and its steady state level increases upon irradiation. In silico analysis of the TbWC-1 sensor domain (LOV) supports the hypothesis that TbWC-1 is a photoreceptor, while the absence of the two polyglutamine regions involved in transcriptional activation in
Neurospora suggests that this function in
Tuber could be lost.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15275664</pmid><doi>10.1016/j.fgb.2004.02.004</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1087-1845 |
| ispartof | Fungal genetics and biology, 2004-07, Vol.41 (7), p.688-697 |
| issn | 1087-1845 1096-0937 |
| language | eng |
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| subjects | Amino Acid Sequence Ascomycota - cytology Ascomycota - genetics Ascomycota - growth & development Blue light Cloning, Molecular Conserved Sequence DNA, Fungal - chemistry DNA, Fungal - isolation & purification DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - isolation & purification Fungal Proteins - chemistry Fungal Proteins - genetics Fungal Proteins - isolation & purification Gene Expression Regulation, Fungal Genes, Fungal Hypogeous life Light Models, Molecular Molecular Sequence Data Morphogenesis Mycelium - genetics Mycelium - growth & development Neurospora crassa - genetics Photomorphogenesis Photoreceptor Photoreceptors, Microbial - genetics Photoreceptors, Microbial - isolation & purification Polyglutamic Acid - genetics Protein Structure, Tertiary RNA, Fungal - analysis RNA, Messenger - analysis Sequence Alignment Sequence Homology, Amino Acid Signal transduction Transcription Factors - chemistry Transcription Factors - genetics Transcription Factors - isolation & purification Transcription, Genetic Tuber borchii White collar-1 |
| title | Photomorphogenesis in the hypogeous fungus Tuber borchii: isolation and characterization of Tbwc-1, the homologue of the blue-light photoreceptor of Neurospora crassa |
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