A rapid flow cytometry assay for the assessment of calcium mobilization in human neutrophils in a small volume of lysed whole-blood
Flow cytometry-based methods have been developed to measure most neutrophil responses. The assessment of the mobilization of calcium, however, is routinely performed on neutrophils isolated from whole blood. This report describes a flow cytometry-based assay to measure the mobilization of calcium in...
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| Veröffentlicht in: | Journal of immunological methods 2009-01, Vol.340 (2), p.154-157 |
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| container_title | Journal of immunological methods |
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| creator | Desmeules, Philippe Dufour, Maurice Fernandes, Maria J.G. |
| description | Flow cytometry-based methods have been developed to measure most neutrophil responses. The assessment of the mobilization of calcium, however, is routinely performed on neutrophils isolated from whole blood. This report describes a flow cytometry-based assay to measure the mobilization of calcium in neutrophils directly in whole blood. This method requires minimal sample manipulation, small volumes of blood and is performed in a short period of time. Both clinical and research laboratories will be able to assess neutrophil function and the quality of granulocyte preparations using a more time and cost effective calcium mobilization test. |
| doi_str_mv | 10.1016/j.jim.2008.10.006 |
| format | Article |
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The assessment of the mobilization of calcium, however, is routinely performed on neutrophils isolated from whole blood. This report describes a flow cytometry-based assay to measure the mobilization of calcium in neutrophils directly in whole blood. This method requires minimal sample manipulation, small volumes of blood and is performed in a short period of time. Both clinical and research laboratories will be able to assess neutrophil function and the quality of granulocyte preparations using a more time and cost effective calcium mobilization test.</description><subject>Biological and medical sciences</subject><subject>Blood Cells - metabolism</subject><subject>Calcium - analysis</subject><subject>Calcium - metabolism</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Indo-1</subject><subject>Intracellular calcium</subject><subject>Kinetics</subject><subject>Molecular immunology</subject><subject>N-Formylmethionine Leucyl-Phenylalanine</subject><subject>Neutrophils</subject><subject>Stimulation</subject><subject>Techniques</subject><subject>Time Factors</subject><subject>Zymosan</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTuP1DAUhS0EYoeFH0CD3ECX4dpOnERUqxUvaSUaqC3HvtF4ZMeD7ewq2_LHSTQj6KC6D33nyj6HkNcM9gyYfH_cH13Yc4BunfcA8gnZsa7lVdtD85TsADivWNv0V-RFzkcAYCDhObli_drLnu3Irxua9MlZOvr4QM1SYsCSFqpz1gsdY6LlgNuEOQecCo0jNdobNwca4uC8e9TFxYm6iR7moCc64VxSPB2cz9tS0xy09_Q--jngJvdLRksfDtFjNfgY7UvybNQ-46tLvSY_Pn38fvuluvv2-evtzV1lasZL1Q8MBi6RSawbY7isTTvUWtcSrDRCGolN3emuHVs7SiFBCtsNQne6bTpha3FN3p3vnlL8OWMuKrhs0Hs9YZyzkrKtOW_Ff0EOXDZ1z1eQnUGTYs4JR3VKLui0KAZqi0gd1RqR2iLaVqvpq-bN5fg8BLR_FZdMVuDtBdB5tXpMejIu_-E4AyE7sX3nw5nD1bN7h0ll43AyaF1CU5SN7h_P-A1MCrAC</recordid><startdate>20090130</startdate><enddate>20090130</enddate><creator>Desmeules, Philippe</creator><creator>Dufour, Maurice</creator><creator>Fernandes, Maria J.G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090130</creationdate><title>A rapid flow cytometry assay for the assessment of calcium mobilization in human neutrophils in a small volume of lysed whole-blood</title><author>Desmeules, Philippe ; Dufour, Maurice ; Fernandes, Maria J.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-9b10b26e16e45cc264c7b4aa460d6c36c6e548a87f7df636063d8b3a8a7583d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biological and medical sciences</topic><topic>Blood Cells - metabolism</topic><topic>Calcium - analysis</topic><topic>Calcium - metabolism</topic><topic>Flow cytometry</topic><topic>Flow Cytometry - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Indo-1</topic><topic>Intracellular calcium</topic><topic>Kinetics</topic><topic>Molecular immunology</topic><topic>N-Formylmethionine Leucyl-Phenylalanine</topic><topic>Neutrophils</topic><topic>Stimulation</topic><topic>Techniques</topic><topic>Time Factors</topic><topic>Zymosan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Desmeules, Philippe</creatorcontrib><creatorcontrib>Dufour, Maurice</creatorcontrib><creatorcontrib>Fernandes, Maria J.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Desmeules, Philippe</au><au>Dufour, Maurice</au><au>Fernandes, Maria J.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid flow cytometry assay for the assessment of calcium mobilization in human neutrophils in a small volume of lysed whole-blood</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2009-01-30</date><risdate>2009</risdate><volume>340</volume><issue>2</issue><spage>154</spage><epage>157</epage><pages>154-157</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>Flow cytometry-based methods have been developed to measure most neutrophil responses. 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| ispartof | Journal of immunological methods, 2009-01, Vol.340 (2), p.154-157 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Blood Cells - metabolism Calcium - analysis Calcium - metabolism Flow cytometry Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Indo-1 Intracellular calcium Kinetics Molecular immunology N-Formylmethionine Leucyl-Phenylalanine Neutrophils Stimulation Techniques Time Factors Zymosan |
| title | A rapid flow cytometry assay for the assessment of calcium mobilization in human neutrophils in a small volume of lysed whole-blood |
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