Mechanical Strain Delivers Anti-apoptotic and Proliferative Signals to Gingival Fibroblasts
Physical forces play a critical role in the survival and proliferation of many cell types, including fibroblasts. Gingival fibroblasts are exposed to mechanical stress during mastication, orthodontic tooth movement, and wound healing following periodontal surgery. The aim of this study was to examin...
Gespeichert in:
| Veröffentlicht in: | Journal of dental research 2004-08, Vol.83 (8), p.596-601 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 601 |
|---|---|
| container_issue | 8 |
| container_start_page | 596 |
| container_title | Journal of dental research |
| container_volume | 83 |
| creator | Danciu, T.E. Gagari, E. Adam, R.M. Damoulis, P.D. Freeman, M.R. |
| description | Physical forces play a critical role in the survival and proliferation of many cell types, including fibroblasts. Gingival fibroblasts are exposed to mechanical stress during mastication, orthodontic tooth movement, and wound healing following periodontal surgery. The aim of this study was to examine the effect of mechanical strain on human gingival fibroblasts (hGF). Cells were subjected to short-term (up to 60 min) and long-term (up to 48 hrs) 20% average elongation at 0.1 Hz. We monitored survival signaling by evaluating the phosphorylation status and localization of Forkhead box (FoxO) family members, which are mediators of apoptosis. We also examined strain-induced proliferation by measuring the level of proliferating cell nuclear antigen (PCNA). We observed that cyclic strain caused the phosphorylation and retention in the cytoplasm of FoxO family members. Moreover, mechanical strain resulted in increased ERK kinase phosphorylation and PCNA expression. In conclusion, cyclic strain delivers anti-apoptotic and proliferative stimuli to hGF. |
| doi_str_mv | 10.1177/154405910408300803 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66741586</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_154405910408300803</sage_id><sourcerecordid>66741586</sourcerecordid><originalsourceid>FETCH-LOGICAL-c461t-9b127c173770d615fb3a8f01e91968003f9d4fbbb98e7eb41db38c84932445e03</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMoun78AQ9SPHirO9MkTXIUv0FRWD15KEmbrpFusybZBf-9XXZBUPA08M4z78y8hBwjnCMKMUbOGHCFwEBSAAl0i4xWYr5St8kIoChyoIzvkf0YPwBQFZLukj3khUBVliPy9mjrd927WnfZJAXt-uzKdm5pQ8wu-uRyPffz5JOrM9032XPwnWtt0GlAsomb9rqLWfLZreunbjmY3DgTvOl0TPGQ7LRD2x5t6gF5vbl-ubzLH55u7y8vHvKalZhyZbAQNQoqBDQl8tZQLVtAq4YTJQBtVcNaY4ySVljDsDFU1pIpWjDGLdADcrb2nQf_ubAxVTMXa9t1urd-EauyFAy5LAfw9Bf44Rdh9UJVgGIcleQDVKyhOvgYg22reXAzHb4qhGqVe_U392HoZOO8MDPb_Ixsgh6A8RqIemp_1v5j-Q1o1InZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>209451985</pqid></control><display><type>article</type><title>Mechanical Strain Delivers Anti-apoptotic and Proliferative Signals to Gingival Fibroblasts</title><source>MEDLINE</source><source>SAGE Complete</source><creator>Danciu, T.E. ; Gagari, E. ; Adam, R.M. ; Damoulis, P.D. ; Freeman, M.R.</creator><creatorcontrib>Danciu, T.E. ; Gagari, E. ; Adam, R.M. ; Damoulis, P.D. ; Freeman, M.R.</creatorcontrib><description>Physical forces play a critical role in the survival and proliferation of many cell types, including fibroblasts. Gingival fibroblasts are exposed to mechanical stress during mastication, orthodontic tooth movement, and wound healing following periodontal surgery. The aim of this study was to examine the effect of mechanical strain on human gingival fibroblasts (hGF). Cells were subjected to short-term (up to 60 min) and long-term (up to 48 hrs) 20% average elongation at 0.1 Hz. We monitored survival signaling by evaluating the phosphorylation status and localization of Forkhead box (FoxO) family members, which are mediators of apoptosis. We also examined strain-induced proliferation by measuring the level of proliferating cell nuclear antigen (PCNA). We observed that cyclic strain caused the phosphorylation and retention in the cytoplasm of FoxO family members. Moreover, mechanical strain resulted in increased ERK kinase phosphorylation and PCNA expression. In conclusion, cyclic strain delivers anti-apoptotic and proliferative stimuli to hGF.</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/154405910408300803</identifier><identifier>PMID: 15271966</identifier><identifier>CODEN: JDREAF</identifier><language>eng</language><publisher>United States: SAGE Publications</publisher><subject>Apoptosis - physiology ; Cell Division - physiology ; Cell Size - physiology ; Cells, Cultured ; Dentistry ; DNA-Binding Proteins - metabolism ; Fibroblasts - metabolism ; Forkhead Box Protein O1 ; Forkhead Transcription Factors ; Gingiva - cytology ; Gingiva - metabolism ; Humans ; Mastication ; Mitogen-Activated Protein Kinases - metabolism ; Proliferating Cell Nuclear Antigen - metabolism ; Protein-Serine-Threonine Kinases - metabolism ; Proto-Oncogene Proteins - metabolism ; Proto-Oncogene Proteins c-akt ; Signal Transduction - physiology ; Stress, Mechanical ; Time Factors ; Transcription Factors - metabolism ; Translocation, Genetic</subject><ispartof>Journal of dental research, 2004-08, Vol.83 (8), p.596-601</ispartof><rights>International and American Associations for Dental Research</rights><rights>Copyright American Association for Dental Research/American Academy of Implant Dentistry Aug 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-9b127c173770d615fb3a8f01e91968003f9d4fbbb98e7eb41db38c84932445e03</citedby><cites>FETCH-LOGICAL-c461t-9b127c173770d615fb3a8f01e91968003f9d4fbbb98e7eb41db38c84932445e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/154405910408300803$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/154405910408300803$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15271966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Danciu, T.E.</creatorcontrib><creatorcontrib>Gagari, E.</creatorcontrib><creatorcontrib>Adam, R.M.</creatorcontrib><creatorcontrib>Damoulis, P.D.</creatorcontrib><creatorcontrib>Freeman, M.R.</creatorcontrib><title>Mechanical Strain Delivers Anti-apoptotic and Proliferative Signals to Gingival Fibroblasts</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Physical forces play a critical role in the survival and proliferation of many cell types, including fibroblasts. Gingival fibroblasts are exposed to mechanical stress during mastication, orthodontic tooth movement, and wound healing following periodontal surgery. The aim of this study was to examine the effect of mechanical strain on human gingival fibroblasts (hGF). Cells were subjected to short-term (up to 60 min) and long-term (up to 48 hrs) 20% average elongation at 0.1 Hz. We monitored survival signaling by evaluating the phosphorylation status and localization of Forkhead box (FoxO) family members, which are mediators of apoptosis. We also examined strain-induced proliferation by measuring the level of proliferating cell nuclear antigen (PCNA). We observed that cyclic strain caused the phosphorylation and retention in the cytoplasm of FoxO family members. Moreover, mechanical strain resulted in increased ERK kinase phosphorylation and PCNA expression. In conclusion, cyclic strain delivers anti-apoptotic and proliferative stimuli to hGF.</description><subject>Apoptosis - physiology</subject><subject>Cell Division - physiology</subject><subject>Cell Size - physiology</subject><subject>Cells, Cultured</subject><subject>Dentistry</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Fibroblasts - metabolism</subject><subject>Forkhead Box Protein O1</subject><subject>Forkhead Transcription Factors</subject><subject>Gingiva - cytology</subject><subject>Gingiva - metabolism</subject><subject>Humans</subject><subject>Mastication</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Proliferating Cell Nuclear Antigen - metabolism</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Proto-Oncogene Proteins c-akt</subject><subject>Signal Transduction - physiology</subject><subject>Stress, Mechanical</subject><subject>Time Factors</subject><subject>Transcription Factors - metabolism</subject><subject>Translocation, Genetic</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kE1LxDAQhoMoun78AQ9SPHirO9MkTXIUv0FRWD15KEmbrpFusybZBf-9XXZBUPA08M4z78y8hBwjnCMKMUbOGHCFwEBSAAl0i4xWYr5St8kIoChyoIzvkf0YPwBQFZLukj3khUBVliPy9mjrd927WnfZJAXt-uzKdm5pQ8wu-uRyPffz5JOrM9032XPwnWtt0GlAsomb9rqLWfLZreunbjmY3DgTvOl0TPGQ7LRD2x5t6gF5vbl-ubzLH55u7y8vHvKalZhyZbAQNQoqBDQl8tZQLVtAq4YTJQBtVcNaY4ySVljDsDFU1pIpWjDGLdADcrb2nQf_ubAxVTMXa9t1urd-EauyFAy5LAfw9Bf44Rdh9UJVgGIcleQDVKyhOvgYg22reXAzHb4qhGqVe_U392HoZOO8MDPb_Ixsgh6A8RqIemp_1v5j-Q1o1InZ</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Danciu, T.E.</creator><creator>Gagari, E.</creator><creator>Adam, R.M.</creator><creator>Damoulis, P.D.</creator><creator>Freeman, M.R.</creator><general>SAGE Publications</general><general>SAGE PUBLICATIONS, INC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Mechanical Strain Delivers Anti-apoptotic and Proliferative Signals to Gingival Fibroblasts</title><author>Danciu, T.E. ; Gagari, E. ; Adam, R.M. ; Damoulis, P.D. ; Freeman, M.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-9b127c173770d615fb3a8f01e91968003f9d4fbbb98e7eb41db38c84932445e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Apoptosis - physiology</topic><topic>Cell Division - physiology</topic><topic>Cell Size - physiology</topic><topic>Cells, Cultured</topic><topic>Dentistry</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Fibroblasts - metabolism</topic><topic>Forkhead Box Protein O1</topic><topic>Forkhead Transcription Factors</topic><topic>Gingiva - cytology</topic><topic>Gingiva - metabolism</topic><topic>Humans</topic><topic>Mastication</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Proliferating Cell Nuclear Antigen - metabolism</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Proto-Oncogene Proteins c-akt</topic><topic>Signal Transduction - physiology</topic><topic>Stress, Mechanical</topic><topic>Time Factors</topic><topic>Transcription Factors - metabolism</topic><topic>Translocation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Danciu, T.E.</creatorcontrib><creatorcontrib>Gagari, E.</creatorcontrib><creatorcontrib>Adam, R.M.</creatorcontrib><creatorcontrib>Damoulis, P.D.</creatorcontrib><creatorcontrib>Freeman, M.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Danciu, T.E.</au><au>Gagari, E.</au><au>Adam, R.M.</au><au>Damoulis, P.D.</au><au>Freeman, M.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanical Strain Delivers Anti-apoptotic and Proliferative Signals to Gingival Fibroblasts</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>83</volume><issue>8</issue><spage>596</spage><epage>601</epage><pages>596-601</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><coden>JDREAF</coden><abstract>Physical forces play a critical role in the survival and proliferation of many cell types, including fibroblasts. Gingival fibroblasts are exposed to mechanical stress during mastication, orthodontic tooth movement, and wound healing following periodontal surgery. The aim of this study was to examine the effect of mechanical strain on human gingival fibroblasts (hGF). Cells were subjected to short-term (up to 60 min) and long-term (up to 48 hrs) 20% average elongation at 0.1 Hz. We monitored survival signaling by evaluating the phosphorylation status and localization of Forkhead box (FoxO) family members, which are mediators of apoptosis. We also examined strain-induced proliferation by measuring the level of proliferating cell nuclear antigen (PCNA). We observed that cyclic strain caused the phosphorylation and retention in the cytoplasm of FoxO family members. Moreover, mechanical strain resulted in increased ERK kinase phosphorylation and PCNA expression. In conclusion, cyclic strain delivers anti-apoptotic and proliferative stimuli to hGF.</abstract><cop>United States</cop><pub>SAGE Publications</pub><pmid>15271966</pmid><doi>10.1177/154405910408300803</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-0345 |
| ispartof | Journal of dental research, 2004-08, Vol.83 (8), p.596-601 |
| issn | 0022-0345 1544-0591 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66741586 |
| source | MEDLINE; SAGE Complete |
| subjects | Apoptosis - physiology Cell Division - physiology Cell Size - physiology Cells, Cultured Dentistry DNA-Binding Proteins - metabolism Fibroblasts - metabolism Forkhead Box Protein O1 Forkhead Transcription Factors Gingiva - cytology Gingiva - metabolism Humans Mastication Mitogen-Activated Protein Kinases - metabolism Proliferating Cell Nuclear Antigen - metabolism Protein-Serine-Threonine Kinases - metabolism Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-akt Signal Transduction - physiology Stress, Mechanical Time Factors Transcription Factors - metabolism Translocation, Genetic |
| title | Mechanical Strain Delivers Anti-apoptotic and Proliferative Signals to Gingival Fibroblasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T20%3A05%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mechanical%20Strain%20Delivers%20Anti-apoptotic%20and%20Proliferative%20Signals%20to%20Gingival%20Fibroblasts&rft.jtitle=Journal%20of%20dental%20research&rft.au=Danciu,%20T.E.&rft.date=2004-08-01&rft.volume=83&rft.issue=8&rft.spage=596&rft.epage=601&rft.pages=596-601&rft.issn=0022-0345&rft.eissn=1544-0591&rft.coden=JDREAF&rft_id=info:doi/10.1177/154405910408300803&rft_dat=%3Cproquest_cross%3E66741586%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=209451985&rft_id=info:pmid/15271966&rft_sage_id=10.1177_154405910408300803&rfr_iscdi=true |