Basement Membrane-Dependent Modification of Phenotype and Gene Expression in Human Retinal Pigment Epithelial ARPE-19 Cells

To use porcine lens capsule (PLC) as basement membrane for ARPE-19 cells and to characterize its effects on cell differentiation and gene expression. Postconfluent cultures of ARPE-19 cells were established on either porous polyester filters or PLC membranes and characterized by electron microscopy,...

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Veröffentlicht in:	Investigative ophthalmology & visual science 2004-08, Vol.45 (8), p.2786-2794
Hauptverfasser:	Turowski, Patric, Adamson, Peter, Sathia, Julie, Zhang, Jin Jun, Moss, Stephen E, Aylward, G. William, Hayes, Matthew J, Kanuga, Naheed, Greenwood, John
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Basement Membrane - physiology Biological and medical sciences Blotting, Northern Cell Culture Techniques - methods Cell Differentiation - physiology Cell Line Cell Polarity - physiology Cell Survival - physiology Electric Conductivity Epistemology Eye and associated structures. Visual pathways and centers. Vision Eye Proteins - genetics Eye Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation - physiology Gene Library Generalities Humans Immunohistochemistry Lens Capsule, Crystalline - physiology Membrane Proteins - genetics Membrane Proteins - metabolism Methodology and general studies Microscopy, Confocal Microscopy, Electron Occludin Organisation and history of research Phagocytosis - physiology Phenotype Phosphoproteins - genetics Phosphoproteins - metabolism Pigment Epithelium of Eye - cytology Pigment Epithelium of Eye - metabolism Prehistory and protohistory RNA, Messenger - metabolism Rod Cell Outer Segment - physiology Swine Vertebrates: nervous system and sense organs Zonula Occludens-1 Protein
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