Cell Survival within Pulp and Periodontal Constructs
Abstract The purpose of this study was to measure cell survival and degradation within tissue-engineered dental constructs. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PLSCs) were seeded on three types of tissue engineering scaffolds: a synthetic open-cell D,D-L,L-polylactic...
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| Veröffentlicht in: | Journal of endodontics 2009-01, Vol.35 (1), p.63-66 |
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| container_title | Journal of endodontics |
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| creator | Gebhardt, Matthew, DDS Murray, Peter E., PhD Namerow, Kenneth N., DDS Kuttler, Sergio, DDS Garcia-Godoy, Franklin, DDS, MS |
| description | Abstract The purpose of this study was to measure cell survival and degradation within tissue-engineered dental constructs. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PLSCs) were seeded on three types of tissue engineering scaffolds: a synthetic open-cell D,D-L,L-polylactic acid (polymer) scaffold, a bovine collagen scaffold (collagen), and a calcium phosphate bioceramic (calcium phosphate) scaffold. The dental pulp and periodontal constructs ( n = 144) were maintained in cell culture for between 3 and 14 days. The cell survival and degradation within the constructs were measured using histologic criteria. The DPSC and PLSC survival was optimal in the polymer and collagen constructs but not the calcium phosphate constructs, especially over longer time periods. These in vitro results suggest that both the polymer and collagen scaffolds and the DPSCs and PLSCs can be combined to create pulp and periodontal constructs for use in future regenerative dental treatments. |
| doi_str_mv | 10.1016/j.joen.2008.09.020 |
| format | Article |
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Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PLSCs) were seeded on three types of tissue engineering scaffolds: a synthetic open-cell D,D-L,L-polylactic acid (polymer) scaffold, a bovine collagen scaffold (collagen), and a calcium phosphate bioceramic (calcium phosphate) scaffold. The dental pulp and periodontal constructs ( n = 144) were maintained in cell culture for between 3 and 14 days. The cell survival and degradation within the constructs were measured using histologic criteria. The DPSC and PLSC survival was optimal in the polymer and collagen constructs but not the calcium phosphate constructs, especially over longer time periods. These in vitro results suggest that both the polymer and collagen scaffolds and the DPSCs and PLSCs can be combined to create pulp and periodontal constructs for use in future regenerative dental treatments.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2008.09.020</identifier><identifier>PMID: 19084127</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult Stem Cells - physiology ; Animals ; Calcium Phosphates ; Cattle ; Cell Culture Techniques ; Cell Survival ; Cells, Cultured ; Collagen ; Dental Pulp - cytology ; Dentistry ; Endocrinology & Metabolism ; Endodontics ; Humans ; L Cells (Cell Line) ; Lactic Acid ; Materials Testing ; Mice ; Periodontal Ligament - cytology ; Polyesters ; Polymers ; regenerative ; scaffolds ; stem cells ; tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry</subject><ispartof>Journal of endodontics, 2009-01, Vol.35 (1), p.63-66</ispartof><rights>American Association of Endodontists</rights><rights>2008 American Association of Endodontists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-b0385982db939dafd904ef2101d165017ca6c853f6b98ac94009fb3d1bf476133</citedby><cites>FETCH-LOGICAL-c475t-b0385982db939dafd904ef2101d165017ca6c853f6b98ac94009fb3d1bf476133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.joen.2008.09.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19084127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gebhardt, Matthew, DDS</creatorcontrib><creatorcontrib>Murray, Peter E., PhD</creatorcontrib><creatorcontrib>Namerow, Kenneth N., DDS</creatorcontrib><creatorcontrib>Kuttler, Sergio, DDS</creatorcontrib><creatorcontrib>Garcia-Godoy, Franklin, DDS, MS</creatorcontrib><title>Cell Survival within Pulp and Periodontal Constructs</title><title>Journal of endodontics</title><addtitle>J Endod</addtitle><description>Abstract The purpose of this study was to measure cell survival and degradation within tissue-engineered dental constructs. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PLSCs) were seeded on three types of tissue engineering scaffolds: a synthetic open-cell D,D-L,L-polylactic acid (polymer) scaffold, a bovine collagen scaffold (collagen), and a calcium phosphate bioceramic (calcium phosphate) scaffold. The dental pulp and periodontal constructs ( n = 144) were maintained in cell culture for between 3 and 14 days. The cell survival and degradation within the constructs were measured using histologic criteria. The DPSC and PLSC survival was optimal in the polymer and collagen constructs but not the calcium phosphate constructs, especially over longer time periods. These in vitro results suggest that both the polymer and collagen scaffolds and the DPSCs and PLSCs can be combined to create pulp and periodontal constructs for use in future regenerative dental treatments.</description><subject>Adult Stem Cells - physiology</subject><subject>Animals</subject><subject>Calcium Phosphates</subject><subject>Cattle</subject><subject>Cell Culture Techniques</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>Dental Pulp - cytology</subject><subject>Dentistry</subject><subject>Endocrinology & Metabolism</subject><subject>Endodontics</subject><subject>Humans</subject><subject>L Cells (Cell Line)</subject><subject>Lactic Acid</subject><subject>Materials Testing</subject><subject>Mice</subject><subject>Periodontal Ligament - cytology</subject><subject>Polyesters</subject><subject>Polymers</subject><subject>regenerative</subject><subject>scaffolds</subject><subject>stem cells</subject><subject>tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kVFr2zAUhcXoWNJsf6APw099s3dl2ZYEo1BCtw4CC2R7FrIkM7mOlEp2Sv79ZBIo9KFP9-Geczj3uwjdYCgw4OZbX_TeuKIEYAXwAkr4gJaYUZaTuq6u0BKA87wknC_QdYw9AKaE0E9ogTmwCpd0iaq1GYZsN4WjPcohe7HjP-uy7TQcMul0tjXBeu3dmHZr7-IYJjXGz-hjJ4dovlzmCv398fBn_Zhvfv_8tb7f5Kqi9Zi3QFjNWalbTriWneZQma5M3TVu6lRGyUaxmnRNy5lUvEp9u5Zo3HYVbTAhK3R7zj0E_zyZOIq9jSoVls74KYqmoYSSqk7C8ixUwccYTCcOwe5lOAkMYmYlejGzEjMrAVwkVsn09ZI-tXujXy0XOEnw_Sww6cajNUFEZY1TRttg1Ci0t-_n372xq8E6q-TwZE4m9n4KLtETWMRSgNjN35qfBSyF0KYm_wHxxI5w</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Gebhardt, Matthew, DDS</creator><creator>Murray, Peter E., PhD</creator><creator>Namerow, Kenneth N., DDS</creator><creator>Kuttler, Sergio, DDS</creator><creator>Garcia-Godoy, Franklin, DDS, MS</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090101</creationdate><title>Cell Survival within Pulp and Periodontal Constructs</title><author>Gebhardt, Matthew, DDS ; Murray, Peter E., PhD ; Namerow, Kenneth N., DDS ; Kuttler, Sergio, DDS ; Garcia-Godoy, Franklin, DDS, MS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-b0385982db939dafd904ef2101d165017ca6c853f6b98ac94009fb3d1bf476133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult Stem Cells - physiology</topic><topic>Animals</topic><topic>Calcium Phosphates</topic><topic>Cattle</topic><topic>Cell Culture Techniques</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Collagen</topic><topic>Dental Pulp - cytology</topic><topic>Dentistry</topic><topic>Endocrinology & Metabolism</topic><topic>Endodontics</topic><topic>Humans</topic><topic>L Cells (Cell Line)</topic><topic>Lactic Acid</topic><topic>Materials Testing</topic><topic>Mice</topic><topic>Periodontal Ligament - cytology</topic><topic>Polyesters</topic><topic>Polymers</topic><topic>regenerative</topic><topic>scaffolds</topic><topic>stem cells</topic><topic>tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gebhardt, Matthew, DDS</creatorcontrib><creatorcontrib>Murray, Peter E., PhD</creatorcontrib><creatorcontrib>Namerow, Kenneth N., DDS</creatorcontrib><creatorcontrib>Kuttler, Sergio, DDS</creatorcontrib><creatorcontrib>Garcia-Godoy, Franklin, DDS, MS</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gebhardt, Matthew, DDS</au><au>Murray, Peter E., PhD</au><au>Namerow, Kenneth N., DDS</au><au>Kuttler, Sergio, DDS</au><au>Garcia-Godoy, Franklin, DDS, MS</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell Survival within Pulp and Periodontal Constructs</atitle><jtitle>Journal of endodontics</jtitle><addtitle>J Endod</addtitle><date>2009-01-01</date><risdate>2009</risdate><volume>35</volume><issue>1</issue><spage>63</spage><epage>66</epage><pages>63-66</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>Abstract The purpose of this study was to measure cell survival and degradation within tissue-engineered dental constructs. 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| ispartof | Journal of endodontics, 2009-01, Vol.35 (1), p.63-66 |
| issn | 0099-2399 1878-3554 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Stem Cells - physiology Animals Calcium Phosphates Cattle Cell Culture Techniques Cell Survival Cells, Cultured Collagen Dental Pulp - cytology Dentistry Endocrinology & Metabolism Endodontics Humans L Cells (Cell Line) Lactic Acid Materials Testing Mice Periodontal Ligament - cytology Polyesters Polymers regenerative scaffolds stem cells tissue engineering Tissue Engineering - methods Tissue Scaffolds - chemistry |
| title | Cell Survival within Pulp and Periodontal Constructs |
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