Substance P Expression by Human Dental Pulp Fibroblasts: A Potential Role in Neurogenic Inflammation
Abstract Neurogenic inflammation describes the local release of neuropeptides, notably substance P (SP), from afferent neurons and might play a role in the pathogenesis of pulpal disease. The fibroblast is the most numerous cell type in the dental pulp, and recent work has suggested that it is invol...
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| Veröffentlicht in: | Journal of endodontics 2009-01, Vol.35 (1), p.73-77 |
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| container_title | Journal of endodontics |
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| creator | Killough, Simon A., BSc, BDS, MFDS, PhD Lundy, Fionnuala T., BSc, PhD Irwin, Chris R., BSc, BDS, FDS (Rest Dent), PhD, FFD |
| description | Abstract Neurogenic inflammation describes the local release of neuropeptides, notably substance P (SP), from afferent neurons and might play a role in the pathogenesis of pulpal disease. The fibroblast is the most numerous cell type in the dental pulp, and recent work has suggested that it is involved in the inflammatory response. Primary pulp fibroblast cell populations were isolated by enzymatic digestion. Whole pulp tissue was obtained from freshly extracted sound (n = 35) and carious (n = 39) teeth. Expression of SP and neurokinin-1 receptor (NK-1) mRNA by pulp fibroblasts was determined by reverse transcriptase polymerase chain reaction (RT-PCR). SP was expressed by pulpal fibroblasts at both mRNA and protein levels. In addition, NK-1 mRNA and protein expression was detected in fibroblast cultures by RT-PCR and Western blotting, respectively. SP levels, determined by radioimmunoassay, were significantly greater ( P < .05) in carious compared with sound teeth. These findings suggest that pulp fibroblasts play a role in neurogenic inflammation in pulpal disease. |
| doi_str_mv | 10.1016/j.joen.2008.10.010 |
| format | Article |
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The fibroblast is the most numerous cell type in the dental pulp, and recent work has suggested that it is involved in the inflammatory response. Primary pulp fibroblast cell populations were isolated by enzymatic digestion. Whole pulp tissue was obtained from freshly extracted sound (n = 35) and carious (n = 39) teeth. Expression of SP and neurokinin-1 receptor (NK-1) mRNA by pulp fibroblasts was determined by reverse transcriptase polymerase chain reaction (RT-PCR). SP was expressed by pulpal fibroblasts at both mRNA and protein levels. In addition, NK-1 mRNA and protein expression was detected in fibroblast cultures by RT-PCR and Western blotting, respectively. SP levels, determined by radioimmunoassay, were significantly greater ( P < .05) in carious compared with sound teeth. These findings suggest that pulp fibroblasts play a role in neurogenic inflammation in pulpal disease.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2008.10.010</identifier><identifier>PMID: 19084129</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Blotting, Western ; Caries ; Cells, Cultured ; Dental Caries - metabolism ; Dental Pulp - cytology ; Dental Pulp - metabolism ; dental pulp fibroblasts ; Dentistry ; Endocrinology & Metabolism ; Fibroblasts - metabolism ; Gene Expression Regulation ; Humans ; Interleukin-1beta - biosynthesis ; neurogenic inflammation ; Neurogenic Inflammation - metabolism ; neurokinin-1 ; Receptors, Neurokinin-1 - biosynthesis ; Reverse Transcriptase Polymerase Chain Reaction ; substance P ; Substance P - biosynthesis ; Transforming Growth Factor beta1 - biosynthesis</subject><ispartof>Journal of endodontics, 2009-01, Vol.35 (1), p.73-77</ispartof><rights>2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-44c4b110f124afb112ec2488c62fd8e43524016f263768a41e768b25bc9abe563</citedby><cites>FETCH-LOGICAL-c475t-44c4b110f124afb112ec2488c62fd8e43524016f263768a41e768b25bc9abe563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.joen.2008.10.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19084129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Killough, Simon A., BSc, BDS, MFDS, PhD</creatorcontrib><creatorcontrib>Lundy, Fionnuala T., BSc, PhD</creatorcontrib><creatorcontrib>Irwin, Chris R., BSc, BDS, FDS (Rest Dent), PhD, FFD</creatorcontrib><title>Substance P Expression by Human Dental Pulp Fibroblasts: A Potential Role in Neurogenic Inflammation</title><title>Journal of endodontics</title><addtitle>J Endod</addtitle><description>Abstract Neurogenic inflammation describes the local release of neuropeptides, notably substance P (SP), from afferent neurons and might play a role in the pathogenesis of pulpal disease. The fibroblast is the most numerous cell type in the dental pulp, and recent work has suggested that it is involved in the inflammatory response. Primary pulp fibroblast cell populations were isolated by enzymatic digestion. Whole pulp tissue was obtained from freshly extracted sound (n = 35) and carious (n = 39) teeth. Expression of SP and neurokinin-1 receptor (NK-1) mRNA by pulp fibroblasts was determined by reverse transcriptase polymerase chain reaction (RT-PCR). SP was expressed by pulpal fibroblasts at both mRNA and protein levels. In addition, NK-1 mRNA and protein expression was detected in fibroblast cultures by RT-PCR and Western blotting, respectively. SP levels, determined by radioimmunoassay, were significantly greater ( P < .05) in carious compared with sound teeth. These findings suggest that pulp fibroblasts play a role in neurogenic inflammation in pulpal disease.</description><subject>Blotting, Western</subject><subject>Caries</subject><subject>Cells, Cultured</subject><subject>Dental Caries - metabolism</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - metabolism</subject><subject>dental pulp fibroblasts</subject><subject>Dentistry</subject><subject>Endocrinology & Metabolism</subject><subject>Fibroblasts - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Interleukin-1beta - biosynthesis</subject><subject>neurogenic inflammation</subject><subject>Neurogenic Inflammation - metabolism</subject><subject>neurokinin-1</subject><subject>Receptors, Neurokinin-1 - biosynthesis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>substance P</subject><subject>Substance P - biosynthesis</subject><subject>Transforming Growth Factor beta1 - biosynthesis</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoMo7rj6BzxITt56zFd3p0WEZd0vWHRw9RySdLWkTSdj0i3Ov980MyB48FRF1VsvVU8h9JqSLSW0eTduxwhhywiRpbAllDxBGypbWfG6Fk_RhpCuqxjvujP0IueRENpy3j5HZ7QjUlDWbVD_sJg862AB7_DVn32CnF0M2Bzw7TLpgD9BmLXHu8Xv8bUzKRqv85zf4wu8i3NputL9Gj1gF_BnWFL8AcFZfBcGr6dJz8XtJXo2aJ_h1Smeo-_XV98ub6v7Lzd3lxf3lRVtPVdCWGEoJQNlQg8lY2CZkNI2bOglCF4zUe4eWMPbRmpBoQTDamM7baBu-Dl6e_Tdp_hrgTyryWUL3usAccmqaVreSNIVITsKbYo5JxjUPrlJp4OiRK1s1ahWtmplu9YK2zL05uS-mAn6vyMnmEXw4SiAcuNvB0ll66Cg7V0CO6s-uv_7f_xn3HpXUGr_Ew6Qx7ikUOgpqjJTRD2s312fS2TJygL8EWwhnxk</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Killough, Simon A., BSc, BDS, MFDS, PhD</creator><creator>Lundy, Fionnuala T., BSc, PhD</creator><creator>Irwin, Chris R., BSc, BDS, FDS (Rest Dent), PhD, FFD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090101</creationdate><title>Substance P Expression by Human Dental Pulp Fibroblasts: A Potential Role in Neurogenic Inflammation</title><author>Killough, Simon A., BSc, BDS, MFDS, PhD ; Lundy, Fionnuala T., BSc, PhD ; Irwin, Chris R., BSc, BDS, FDS (Rest Dent), PhD, FFD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-44c4b110f124afb112ec2488c62fd8e43524016f263768a41e768b25bc9abe563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Blotting, Western</topic><topic>Caries</topic><topic>Cells, Cultured</topic><topic>Dental Caries - metabolism</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - metabolism</topic><topic>dental pulp fibroblasts</topic><topic>Dentistry</topic><topic>Endocrinology & Metabolism</topic><topic>Fibroblasts - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Interleukin-1beta - biosynthesis</topic><topic>neurogenic inflammation</topic><topic>Neurogenic Inflammation - metabolism</topic><topic>neurokinin-1</topic><topic>Receptors, Neurokinin-1 - biosynthesis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>substance P</topic><topic>Substance P - biosynthesis</topic><topic>Transforming Growth Factor beta1 - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Killough, Simon A., BSc, BDS, MFDS, PhD</creatorcontrib><creatorcontrib>Lundy, Fionnuala T., BSc, PhD</creatorcontrib><creatorcontrib>Irwin, Chris R., BSc, BDS, FDS (Rest Dent), PhD, FFD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Killough, Simon A., BSc, BDS, MFDS, PhD</au><au>Lundy, Fionnuala T., BSc, PhD</au><au>Irwin, Chris R., BSc, BDS, FDS (Rest Dent), PhD, FFD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Substance P Expression by Human Dental Pulp Fibroblasts: A Potential Role in Neurogenic Inflammation</atitle><jtitle>Journal of endodontics</jtitle><addtitle>J Endod</addtitle><date>2009-01-01</date><risdate>2009</risdate><volume>35</volume><issue>1</issue><spage>73</spage><epage>77</epage><pages>73-77</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>Abstract Neurogenic inflammation describes the local release of neuropeptides, notably substance P (SP), from afferent neurons and might play a role in the pathogenesis of pulpal disease. The fibroblast is the most numerous cell type in the dental pulp, and recent work has suggested that it is involved in the inflammatory response. Primary pulp fibroblast cell populations were isolated by enzymatic digestion. Whole pulp tissue was obtained from freshly extracted sound (n = 35) and carious (n = 39) teeth. Expression of SP and neurokinin-1 receptor (NK-1) mRNA by pulp fibroblasts was determined by reverse transcriptase polymerase chain reaction (RT-PCR). SP was expressed by pulpal fibroblasts at both mRNA and protein levels. In addition, NK-1 mRNA and protein expression was detected in fibroblast cultures by RT-PCR and Western blotting, respectively. SP levels, determined by radioimmunoassay, were significantly greater ( P < .05) in carious compared with sound teeth. These findings suggest that pulp fibroblasts play a role in neurogenic inflammation in pulpal disease.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19084129</pmid><doi>10.1016/j.joen.2008.10.010</doi><tpages>5</tpages></addata></record> |
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| subjects | Blotting, Western Caries Cells, Cultured Dental Caries - metabolism Dental Pulp - cytology Dental Pulp - metabolism dental pulp fibroblasts Dentistry Endocrinology & Metabolism Fibroblasts - metabolism Gene Expression Regulation Humans Interleukin-1beta - biosynthesis neurogenic inflammation Neurogenic Inflammation - metabolism neurokinin-1 Receptors, Neurokinin-1 - biosynthesis Reverse Transcriptase Polymerase Chain Reaction substance P Substance P - biosynthesis Transforming Growth Factor beta1 - biosynthesis |
| title | Substance P Expression by Human Dental Pulp Fibroblasts: A Potential Role in Neurogenic Inflammation |
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