Is the G72/G30 locus associated with schizophrenia? single nucleotide polymorphisms, haplotypes, and gene expression analysis
The genes G72/G30 were recently implicated in schizophrenia in both Canadian and Russian populations. We hypothesized that 1) polymorphic changes in this gene region might be associated with schizophrenia in the Ashkenazi Jewish population and that 2) changes in G72/G30 gene expression might be expe...
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| Veröffentlicht in: | Biological psychiatry (1969) 2004-08, Vol.56 (3), p.169-176 |
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| container_title | Biological psychiatry (1969) |
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| creator | Korostishevsky, Michael Kaganovich, Miryam Cholostoy, Alina Ashkenazi, Maya Ratner, Yael Dahary, Dvir Bernstein, Jeanne Bening-Abu-Shach, Ullrike Ben-Asher, Edna Lancet, Doron Ritsner, Michael Navon, Ruth |
| description | The genes G72/G30 were recently implicated in schizophrenia in both Canadian and Russian populations. We hypothesized that 1) polymorphic changes in this gene region might be associated with schizophrenia in the Ashkenazi Jewish population and that 2) changes in G72/G30 gene expression might be expected in schizophrenic patients compared with control subjects.
Eleven single nucleotide polymorphisms (SNPs) encompassing the G72/G30 genes were typed in the genomic deoxyribonucleic acid (DNA) from 60 schizophrenic patients and 130 matched control subjects of Ashkenazi ethnic origin. Case–control comparisons were based on linkage disequilibrium (LD) and haplotype frequency estimations. Gene expression analysis of G72 and G30 was performed on 88 postmortem dorsolateral prefrontal cortex samples.
Linkage disequilibrium analysis revealed two main SNP blocks. Haplotype analysis on block II, containing three SNPs external to the genes, demonstrated an association with schizophrenia. Gene expression analysis exhibited correlations between expression levels of the G72 and G30 genes, as well as a tendency toward overexpression of the G72 gene in schizophrenic brain samples of 44 schizophrenic patients compared with 44 control subjects.
It is likely that the G72/G30 region is involved in susceptibility to schizophrenia in the Ashkenazi population. The elevation in expression of the G72 gene coincides with the glutamatergic theory of schizophrenia. |
| doi_str_mv | 10.1016/j.biopsych.2004.04.006 |
| format | Article |
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Eleven single nucleotide polymorphisms (SNPs) encompassing the G72/G30 genes were typed in the genomic deoxyribonucleic acid (DNA) from 60 schizophrenic patients and 130 matched control subjects of Ashkenazi ethnic origin. Case–control comparisons were based on linkage disequilibrium (LD) and haplotype frequency estimations. Gene expression analysis of G72 and G30 was performed on 88 postmortem dorsolateral prefrontal cortex samples.
Linkage disequilibrium analysis revealed two main SNP blocks. Haplotype analysis on block II, containing three SNPs external to the genes, demonstrated an association with schizophrenia. Gene expression analysis exhibited correlations between expression levels of the G72 and G30 genes, as well as a tendency toward overexpression of the G72 gene in schizophrenic brain samples of 44 schizophrenic patients compared with 44 control subjects.
It is likely that the G72/G30 region is involved in susceptibility to schizophrenia in the Ashkenazi population. The elevation in expression of the G72 gene coincides with the glutamatergic theory of schizophrenia.</description><identifier>ISSN: 0006-3223</identifier><identifier>EISSN: 1873-2402</identifier><identifier>DOI: 10.1016/j.biopsych.2004.04.006</identifier><identifier>PMID: 15271585</identifier><identifier>CODEN: BIPCBF</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Adult and adolescent clinical studies ; Aged ; Ashkenazi Jews ; Biological and medical sciences ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Case-Control Studies ; dorsolateral prefrontal cortex ; European Continental Ancestry Group - ethnology ; European Continental Ancestry Group - genetics ; Female ; Gene Expression - physiology ; Gene Frequency ; Genetic Predisposition to Disease ; Haplotypes - genetics ; Humans ; Jews - ethnology ; Jews - genetics ; Linkage Disequilibrium ; Male ; Medical sciences ; Middle Aged ; Polymorphism, Single Nucleotide ; Postmortem Changes ; Prefrontal Cortex - metabolism ; Proteins - genetics ; Proteins - metabolism ; Psychology. Psychoanalysis. Psychiatry ; Psychopathology. Psychiatry ; Psychoses ; real-time polymerase chain reaction ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Messenger - biosynthesis ; Schizophrenia ; Schizophrenia - genetics ; susceptibility genes</subject><ispartof>Biological psychiatry (1969), 2004-08, Vol.56 (3), p.169-176</ispartof><rights>2004 Society of Biological Psychiatry</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-1c004613a159140fee4db137de34d90f1240a62538fafc6995b5d98a41502a203</citedby><cites>FETCH-LOGICAL-c394t-1c004613a159140fee4db137de34d90f1240a62538fafc6995b5d98a41502a203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006322304004780$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15975704$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15271585$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Korostishevsky, Michael</creatorcontrib><creatorcontrib>Kaganovich, Miryam</creatorcontrib><creatorcontrib>Cholostoy, Alina</creatorcontrib><creatorcontrib>Ashkenazi, Maya</creatorcontrib><creatorcontrib>Ratner, Yael</creatorcontrib><creatorcontrib>Dahary, Dvir</creatorcontrib><creatorcontrib>Bernstein, Jeanne</creatorcontrib><creatorcontrib>Bening-Abu-Shach, Ullrike</creatorcontrib><creatorcontrib>Ben-Asher, Edna</creatorcontrib><creatorcontrib>Lancet, Doron</creatorcontrib><creatorcontrib>Ritsner, Michael</creatorcontrib><creatorcontrib>Navon, Ruth</creatorcontrib><title>Is the G72/G30 locus associated with schizophrenia? single nucleotide polymorphisms, haplotypes, and gene expression analysis</title><title>Biological psychiatry (1969)</title><addtitle>Biol Psychiatry</addtitle><description>The genes G72/G30 were recently implicated in schizophrenia in both Canadian and Russian populations. We hypothesized that 1) polymorphic changes in this gene region might be associated with schizophrenia in the Ashkenazi Jewish population and that 2) changes in G72/G30 gene expression might be expected in schizophrenic patients compared with control subjects.
Eleven single nucleotide polymorphisms (SNPs) encompassing the G72/G30 genes were typed in the genomic deoxyribonucleic acid (DNA) from 60 schizophrenic patients and 130 matched control subjects of Ashkenazi ethnic origin. Case–control comparisons were based on linkage disequilibrium (LD) and haplotype frequency estimations. Gene expression analysis of G72 and G30 was performed on 88 postmortem dorsolateral prefrontal cortex samples.
Linkage disequilibrium analysis revealed two main SNP blocks. Haplotype analysis on block II, containing three SNPs external to the genes, demonstrated an association with schizophrenia. Gene expression analysis exhibited correlations between expression levels of the G72 and G30 genes, as well as a tendency toward overexpression of the G72 gene in schizophrenic brain samples of 44 schizophrenic patients compared with 44 control subjects.
It is likely that the G72/G30 region is involved in susceptibility to schizophrenia in the Ashkenazi population. The elevation in expression of the G72 gene coincides with the glutamatergic theory of schizophrenia.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Adult and adolescent clinical studies</subject><subject>Aged</subject><subject>Ashkenazi Jews</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Case-Control Studies</subject><subject>dorsolateral prefrontal cortex</subject><subject>European Continental Ancestry Group - ethnology</subject><subject>European Continental Ancestry Group - genetics</subject><subject>Female</subject><subject>Gene Expression - physiology</subject><subject>Gene Frequency</subject><subject>Genetic Predisposition to Disease</subject><subject>Haplotypes - genetics</subject><subject>Humans</subject><subject>Jews - ethnology</subject><subject>Jews - genetics</subject><subject>Linkage Disequilibrium</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Postmortem Changes</subject><subject>Prefrontal Cortex - metabolism</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopathology. Psychiatry</subject><subject>Psychoses</subject><subject>real-time polymerase chain reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Schizophrenia</subject><subject>Schizophrenia - genetics</subject><subject>susceptibility genes</subject><issn>0006-3223</issn><issn>1873-2402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEuLFDEQgIMo7rj6F5Zc9GTP5tHPk8rijgsLXvQcMkn1doZ00qa61Rb875thRvQmFCRVfJVUfYRccbbljNfXh-3exQlXM2wFY-X2GKx-Qja8bWQhSiaekg3LpUIKIS_IC8RDThsh-HNywSvR8KqtNuT3HdJ5ALprxPVOMuqjWZBqxGicnsHSH24eKJrB_YrTkCA4_Y6iCw8eaFiMhzg7C3SKfh1jmgaHI76lg558nNcJ8l0HSx8gAIWfUwJEF0Ouab-iw5fkWa89wqvzeUm-3n78cvOpuP-8u7v5cF8Y2ZVzwU1eseZS86rjJesBSrvnsrEgS9uxnud9dS0q2fa6N3XXVfvKdq0uecWEFkxekjend6cUvy2AsxodGvBeB4gLqrpuZNlykcH6BJoUERP0akpu1GlVnKmjeHVQf8Sro3h1DFbnxqvzD8t-BPu37Ww6A6_PgEajfZ90MA7_4bqmaliZufcnDrKP7w6SQuMgGLAugZmVje5_szwC9tql4Q</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Korostishevsky, Michael</creator><creator>Kaganovich, Miryam</creator><creator>Cholostoy, Alina</creator><creator>Ashkenazi, Maya</creator><creator>Ratner, Yael</creator><creator>Dahary, Dvir</creator><creator>Bernstein, Jeanne</creator><creator>Bening-Abu-Shach, Ullrike</creator><creator>Ben-Asher, Edna</creator><creator>Lancet, Doron</creator><creator>Ritsner, Michael</creator><creator>Navon, Ruth</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Is the G72/G30 locus associated with schizophrenia? single nucleotide polymorphisms, haplotypes, and gene expression analysis</title><author>Korostishevsky, Michael ; Kaganovich, Miryam ; Cholostoy, Alina ; Ashkenazi, Maya ; Ratner, Yael ; Dahary, Dvir ; Bernstein, Jeanne ; Bening-Abu-Shach, Ullrike ; Ben-Asher, Edna ; Lancet, Doron ; Ritsner, Michael ; Navon, Ruth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-1c004613a159140fee4db137de34d90f1240a62538fafc6995b5d98a41502a203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Adult and adolescent clinical studies</topic><topic>Aged</topic><topic>Ashkenazi Jews</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Case-Control Studies</topic><topic>dorsolateral prefrontal cortex</topic><topic>European Continental Ancestry Group - ethnology</topic><topic>European Continental Ancestry Group - genetics</topic><topic>Female</topic><topic>Gene Expression - physiology</topic><topic>Gene Frequency</topic><topic>Genetic Predisposition to Disease</topic><topic>Haplotypes - genetics</topic><topic>Humans</topic><topic>Jews - ethnology</topic><topic>Jews - genetics</topic><topic>Linkage Disequilibrium</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Postmortem Changes</topic><topic>Prefrontal Cortex - metabolism</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychopathology. Psychiatry</topic><topic>Psychoses</topic><topic>real-time polymerase chain reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Schizophrenia</topic><topic>Schizophrenia - genetics</topic><topic>susceptibility genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Korostishevsky, Michael</creatorcontrib><creatorcontrib>Kaganovich, Miryam</creatorcontrib><creatorcontrib>Cholostoy, Alina</creatorcontrib><creatorcontrib>Ashkenazi, Maya</creatorcontrib><creatorcontrib>Ratner, Yael</creatorcontrib><creatorcontrib>Dahary, Dvir</creatorcontrib><creatorcontrib>Bernstein, Jeanne</creatorcontrib><creatorcontrib>Bening-Abu-Shach, Ullrike</creatorcontrib><creatorcontrib>Ben-Asher, Edna</creatorcontrib><creatorcontrib>Lancet, Doron</creatorcontrib><creatorcontrib>Ritsner, Michael</creatorcontrib><creatorcontrib>Navon, Ruth</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biological psychiatry (1969)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Korostishevsky, Michael</au><au>Kaganovich, Miryam</au><au>Cholostoy, Alina</au><au>Ashkenazi, Maya</au><au>Ratner, Yael</au><au>Dahary, Dvir</au><au>Bernstein, Jeanne</au><au>Bening-Abu-Shach, Ullrike</au><au>Ben-Asher, Edna</au><au>Lancet, Doron</au><au>Ritsner, Michael</au><au>Navon, Ruth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Is the G72/G30 locus associated with schizophrenia? single nucleotide polymorphisms, haplotypes, and gene expression analysis</atitle><jtitle>Biological psychiatry (1969)</jtitle><addtitle>Biol Psychiatry</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>56</volume><issue>3</issue><spage>169</spage><epage>176</epage><pages>169-176</pages><issn>0006-3223</issn><eissn>1873-2402</eissn><coden>BIPCBF</coden><abstract>The genes G72/G30 were recently implicated in schizophrenia in both Canadian and Russian populations. We hypothesized that 1) polymorphic changes in this gene region might be associated with schizophrenia in the Ashkenazi Jewish population and that 2) changes in G72/G30 gene expression might be expected in schizophrenic patients compared with control subjects.
Eleven single nucleotide polymorphisms (SNPs) encompassing the G72/G30 genes were typed in the genomic deoxyribonucleic acid (DNA) from 60 schizophrenic patients and 130 matched control subjects of Ashkenazi ethnic origin. Case–control comparisons were based on linkage disequilibrium (LD) and haplotype frequency estimations. Gene expression analysis of G72 and G30 was performed on 88 postmortem dorsolateral prefrontal cortex samples.
Linkage disequilibrium analysis revealed two main SNP blocks. Haplotype analysis on block II, containing three SNPs external to the genes, demonstrated an association with schizophrenia. Gene expression analysis exhibited correlations between expression levels of the G72 and G30 genes, as well as a tendency toward overexpression of the G72 gene in schizophrenic brain samples of 44 schizophrenic patients compared with 44 control subjects.
It is likely that the G72/G30 region is involved in susceptibility to schizophrenia in the Ashkenazi population. The elevation in expression of the G72 gene coincides with the glutamatergic theory of schizophrenia.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>15271585</pmid><doi>10.1016/j.biopsych.2004.04.006</doi><tpages>8</tpages></addata></record> |
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| subjects | Adolescent Adult Adult and adolescent clinical studies Aged Ashkenazi Jews Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - metabolism Case-Control Studies dorsolateral prefrontal cortex European Continental Ancestry Group - ethnology European Continental Ancestry Group - genetics Female Gene Expression - physiology Gene Frequency Genetic Predisposition to Disease Haplotypes - genetics Humans Jews - ethnology Jews - genetics Linkage Disequilibrium Male Medical sciences Middle Aged Polymorphism, Single Nucleotide Postmortem Changes Prefrontal Cortex - metabolism Proteins - genetics Proteins - metabolism Psychology. Psychoanalysis. Psychiatry Psychopathology. Psychiatry Psychoses real-time polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - biosynthesis Schizophrenia Schizophrenia - genetics susceptibility genes |
| title | Is the G72/G30 locus associated with schizophrenia? single nucleotide polymorphisms, haplotypes, and gene expression analysis |
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