Biochemical and structural characterization of RHDV capsid protein variants produced in Pichia pastoris: Advantages for immunization strategies and vaccine implementation

Rabbit hemorrhagic disease virus (RHDV) VP60 capsid protein was recently expressed at approximately 1.5 g L −1 associated with the disruption pellet of Pichia pastoris, thus requiring an additional process of extraction by solubilization. Consequently, the expression of a soluble variant of VP60 was...

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Veröffentlicht in:	Antiviral research 2009, Vol.81 (1), p.25-36
Hauptverfasser:	Farnós, Omar, Fernández, Erlinda, Chiong, Maylin, Parra, Francisco, Joglar, Marisdania, Méndez, Lídice, Rodríguez, Elsa, Moya, Galina, Rodríguez, Dalia, Lleonart, Ricardo, González, Ernesto M., Alonso, Alena, Alfonso, Pastor, Suárez, Marisela, Rodríguez, María P., Toledo, Jorge R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antibodies, Viral - blood Antiviral agents Biological and medical sciences Caliciviridae Infections - immunology Caliciviridae Infections - prevention & control Caliciviridae Infections - veterinary Caliciviridae Infections - virology Calicivirus Capsid Proteins - genetics Capsid Proteins - immunology Capsid Proteins - isolation & purification Gene Expression Hemorrhagic Disease Virus, Rabbit - genetics Hemorrhagic Disease Virus, Rabbit - immunology Immunization - veterinary Immunoglobulin G - blood Long-term immunity Medical sciences Multimeric protein Pharmacology. Drug treatments Pichia - genetics Pichia - metabolism Pichia pastoris Rabbit hemorrhagic disease virus Rabbits Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - isolation & purification Recombinant vaccine RHDV Viral Vaccines - economics Viral Vaccines - genetics Viral Vaccines - immunology Viral Vaccines - isolation & purification VLP
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