EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS
In the present study, we compared the effect of 5% oxygen tension (hypoxia) with a normal tension of 21% oxygen (normoxia) on macrophage infection by the protozoan parasite Leishmania amazonensis. Macrophages from different sources (human cell line U937, murine cell line J774, and murine peritoneal...
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| Veröffentlicht in: | The Journal of parasitology 2004-06, Vol.90 (3), p.510-515 |
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| creator | Colhone, Marcelle Carolina Arrais-Silva, Wagner Welber Picoli, Claudia Giorgio, Selma |
| description | In the present study, we compared the effect of 5% oxygen tension (hypoxia) with a normal tension of 21% oxygen (normoxia) on macrophage infection by the protozoan parasite Leishmania amazonensis. Macrophages from different sources (human cell line U937, murine cell line J774, and murine peritoneal macrophages) exposed to hypoxia showed a reduction of the percentage of infected cells and the number of intracellular parasites per cell. Observations on the kinetics of infection indicated that hypoxia did not depress L. amazonensis phagocytosis but induced macrophages to reduce intracellular parasitism. Furthermore, hypoxia did not act synergistically with γ-interferon and bacterial lipopolysaccharides in macrophages to induce killing of parasites. Experiments also indicated no correlation between nitric oxide production and control of infection in macrophages under hypoxic condition. Thus, we have provided the first evidence that hypoxia, which occurs in various pathological conditions, can alter macrophage susceptibility to a parasitic infection. |
| doi_str_mv | 10.1645/GE-3286 |
| format | Article |
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Macrophages from different sources (human cell line U937, murine cell line J774, and murine peritoneal macrophages) exposed to hypoxia showed a reduction of the percentage of infected cells and the number of intracellular parasites per cell. Observations on the kinetics of infection indicated that hypoxia did not depress L. amazonensis phagocytosis but induced macrophages to reduce intracellular parasitism. Furthermore, hypoxia did not act synergistically with γ-interferon and bacterial lipopolysaccharides in macrophages to induce killing of parasites. Experiments also indicated no correlation between nitric oxide production and control of infection in macrophages under hypoxic condition. Thus, we have provided the first evidence that hypoxia, which occurs in various pathological conditions, can alter macrophage susceptibility to a parasitic infection.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.1645/GE-3286</identifier><identifier>PMID: 15270094</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Amastigotes ; Animals ; Antibiotics ; Biological and medical sciences ; Cell culture ; Cell culture techniques ; Cell growth ; Cell Hypoxia ; Cell Line ; Cell Survival ; Cells, Cultured ; Experiments ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Humans ; Hypoxia ; IMMUNOLOGY ; Infections ; Interferon ; Interferon-gamma - pharmacology ; Intracellular ; Intracellular parasitism ; Invertebrates ; Leishmania mexicana - immunology ; Leishmania mexicana - physiology ; Lipopolysaccharides ; Lipopolysaccharides - pharmacology ; Macrophages ; Macrophages, Peritoneal - drug effects ; Macrophages, Peritoneal - immunology ; Macrophages, Peritoneal - parasitology ; Metabolism ; Mice ; Mice, Inbred BALB C ; Microorganisms ; Nitric oxide ; Nitric Oxide - biosynthesis ; Oxides ; Oxygen ; Oxygen tension ; Parasites ; Parasitic diseases ; Parasitism ; Phagocytosis ; U937 Cells ; γ-Interferon</subject><ispartof>The Journal of parasitology, 2004-06, Vol.90 (3), p.510-515</ispartof><rights>American Society of Parasitologists</rights><rights>Copyright 2004 American Society of Parasitologists</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Allen Press Inc. Jun 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b386t-ee21079a01a5df699cf2a196af1d481360d26356e07706eab48594285ead9b6e3</citedby><cites>FETCH-LOGICAL-b386t-ee21079a01a5df699cf2a196af1d481360d26356e07706eab48594285ead9b6e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1645/GE-3286$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3286172$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,26978,27924,27925,52363,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15902357$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15270094$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Colhone, Marcelle Carolina</creatorcontrib><creatorcontrib>Arrais-Silva, Wagner Welber</creatorcontrib><creatorcontrib>Picoli, Claudia</creatorcontrib><creatorcontrib>Giorgio, Selma</creatorcontrib><title>EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>In the present study, we compared the effect of 5% oxygen tension (hypoxia) with a normal tension of 21% oxygen (normoxia) on macrophage infection by the protozoan parasite Leishmania amazonensis. Macrophages from different sources (human cell line U937, murine cell line J774, and murine peritoneal macrophages) exposed to hypoxia showed a reduction of the percentage of infected cells and the number of intracellular parasites per cell. Observations on the kinetics of infection indicated that hypoxia did not depress L. amazonensis phagocytosis but induced macrophages to reduce intracellular parasitism. Furthermore, hypoxia did not act synergistically with γ-interferon and bacterial lipopolysaccharides in macrophages to induce killing of parasites. Experiments also indicated no correlation between nitric oxide production and control of infection in macrophages under hypoxic condition. Thus, we have provided the first evidence that hypoxia, which occurs in various pathological conditions, can alter macrophage susceptibility to a parasitic infection.</description><subject>Amastigotes</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Biological and medical sciences</subject><subject>Cell culture</subject><subject>Cell culture techniques</subject><subject>Cell growth</subject><subject>Cell Hypoxia</subject><subject>Cell Line</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Experiments</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Humans</subject><subject>Hypoxia</subject><subject>IMMUNOLOGY</subject><subject>Infections</subject><subject>Interferon</subject><subject>Interferon-gamma - pharmacology</subject><subject>Intracellular</subject><subject>Intracellular parasitism</subject><subject>Invertebrates</subject><subject>Leishmania mexicana - immunology</subject><subject>Leishmania mexicana - physiology</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Macrophages</subject><subject>Macrophages, Peritoneal - drug effects</subject><subject>Macrophages, Peritoneal - immunology</subject><subject>Macrophages, Peritoneal - parasitology</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microorganisms</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Oxides</subject><subject>Oxygen</subject><subject>Oxygen tension</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Parasitism</subject><subject>Phagocytosis</subject><subject>U937 Cells</subject><subject>γ-Interferon</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp10NFKwzAUBuAgiptTfAGRgqhX1ZOkSZvLWrq1sLXDTXDelLRNYWNbZ7Nd-PZmrDgRvAiBnI8_hx-hawxPmDvseRDalHj8BHWxoK5NqMNOUReAEJtSwTroQusFADBzzlEHM-ICCKeLgrDfD4OplfataDZO32PfShNr5Aev6TjyB6EVJ_t5bB5fZtYwjCfRyE-M8kf-R5qEySSeXKKzSi61umrvHnrrh9MgsofpIA78oZ1Tj29tpQgGV0jAkpUVF6KoiMSCywqXjocph5JwyrgC1wWuZO54TDjEY0qWIueK9tDDIXfT1J87pbfZaq4LtVzKtap3OuPcpY5DwMC7P3BR75q12S0jnDEgTBBs1ONBFU2tdaOqbNPMV7L5yjBk-1azQZjtWzXyts3b5StVHl1bowH3LZC6kMuqketirn85AYQy17ibg1vobd38zPefYJccY_J5Xa_Vv_t8A31bip4</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Colhone, Marcelle Carolina</creator><creator>Arrais-Silva, Wagner Welber</creator><creator>Picoli, Claudia</creator><creator>Giorgio, Selma</creator><general>American Society of Parasitologists</general><general>Allen Press Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7SN</scope><scope>7SS</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040601</creationdate><title>EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS</title><author>Colhone, Marcelle Carolina ; Arrais-Silva, Wagner Welber ; Picoli, Claudia ; Giorgio, Selma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b386t-ee21079a01a5df699cf2a196af1d481360d26356e07706eab48594285ead9b6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amastigotes</topic><topic>Animals</topic><topic>Antibiotics</topic><topic>Biological and medical sciences</topic><topic>Cell culture</topic><topic>Cell culture techniques</topic><topic>Cell growth</topic><topic>Cell Hypoxia</topic><topic>Cell Line</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Experiments</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Humans</topic><topic>Hypoxia</topic><topic>IMMUNOLOGY</topic><topic>Infections</topic><topic>Interferon</topic><topic>Interferon-gamma - pharmacology</topic><topic>Intracellular</topic><topic>Intracellular parasitism</topic><topic>Invertebrates</topic><topic>Leishmania mexicana - immunology</topic><topic>Leishmania mexicana - physiology</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Macrophages</topic><topic>Macrophages, Peritoneal - drug effects</topic><topic>Macrophages, Peritoneal - immunology</topic><topic>Macrophages, Peritoneal - parasitology</topic><topic>Metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microorganisms</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Oxides</topic><topic>Oxygen</topic><topic>Oxygen tension</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>Parasitism</topic><topic>Phagocytosis</topic><topic>U937 Cells</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Colhone, Marcelle Carolina</creatorcontrib><creatorcontrib>Arrais-Silva, Wagner Welber</creatorcontrib><creatorcontrib>Picoli, Claudia</creatorcontrib><creatorcontrib>Giorgio, Selma</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Colhone, Marcelle Carolina</au><au>Arrais-Silva, Wagner Welber</au><au>Picoli, Claudia</au><au>Giorgio, Selma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>90</volume><issue>3</issue><spage>510</spage><epage>515</epage><pages>510-515</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>In the present study, we compared the effect of 5% oxygen tension (hypoxia) with a normal tension of 21% oxygen (normoxia) on macrophage infection by the protozoan parasite Leishmania amazonensis. Macrophages from different sources (human cell line U937, murine cell line J774, and murine peritoneal macrophages) exposed to hypoxia showed a reduction of the percentage of infected cells and the number of intracellular parasites per cell. Observations on the kinetics of infection indicated that hypoxia did not depress L. amazonensis phagocytosis but induced macrophages to reduce intracellular parasitism. Furthermore, hypoxia did not act synergistically with γ-interferon and bacterial lipopolysaccharides in macrophages to induce killing of parasites. Experiments also indicated no correlation between nitric oxide production and control of infection in macrophages under hypoxic condition. Thus, we have provided the first evidence that hypoxia, which occurs in various pathological conditions, can alter macrophage susceptibility to a parasitic infection.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>15270094</pmid><doi>10.1645/GE-3286</doi><tpages>6</tpages></addata></record> |
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| subjects | Amastigotes Animals Antibiotics Biological and medical sciences Cell culture Cell culture techniques Cell growth Cell Hypoxia Cell Line Cell Survival Cells, Cultured Experiments Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Humans Hypoxia IMMUNOLOGY Infections Interferon Interferon-gamma - pharmacology Intracellular Intracellular parasitism Invertebrates Leishmania mexicana - immunology Leishmania mexicana - physiology Lipopolysaccharides Lipopolysaccharides - pharmacology Macrophages Macrophages, Peritoneal - drug effects Macrophages, Peritoneal - immunology Macrophages, Peritoneal - parasitology Metabolism Mice Mice, Inbred BALB C Microorganisms Nitric oxide Nitric Oxide - biosynthesis Oxides Oxygen Oxygen tension Parasites Parasitic diseases Parasitism Phagocytosis U937 Cells γ-Interferon |
| title | EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS |
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